Abstract
Grain weight and quality are always determined by grain filling. Plant microRNAs have drawn attention as key targets for regulation of grain size and yield. However, the mechanisms that underlie grain size regulation remain largely unclear because of the complex networks that control this trait. Our earlier studies demonstrated that suppressed expression of miR167 (STTM/MIM167) substantially increased grain weight. In a field test, the yield increased up to 12.90%-21.94% because of a significantly enhanced grain filling rate. Here, biochemical and genetic analyses revealed the regulatory effects of miR159 on miR167 expression. Further analysis indicated that OsARF12 is the major mediator by which miR167 regulates rice grain filling. Overexpression of OsARF12 produced grain weight and grain filling phenotypes resembling those of STTM/MIM167 plants. Upon in-depth analysis, we found that OsARF12 activates OsCDKF;2 expression by directly binding to the TGTCGG motif in its promoter region. Flow cytometry analysis of young panicles from OsARF12-overexpressing plants and examination of cell number in cdkf;2 mutants verified that OsARF12 positively regulates grain filling and grain size by targeting OsCDKF;2. Moreover, RNA sequencing results suggested that the miR167-OsARF12 module is involved in the cell development process and hormone pathways. OsARF12-overexpressing plants and cdkf;2 mutants exhibited enhanced and reduced sensitivity to exogenous auxin and brassinosteroid (BR) treatment, confirming that targeting of OsCDKF;2 by OsARF12 mediates auxin and BR signaling. Our results reveal that the miR167-OsARF12 module works downstream of miR159 to regulate rice grain filling and grain size via OsCDKF;2 by controlling cell division and mediating auxin and BR signals.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 11
Claim · model-written summary
Suppression of miR167 increased grain weight.
- Taxon
- rice
Oryza sativat0 - Treatment
- suppressed expression of miR167 (STTM/MIM167)e0
- Gene
- miR167e0
- Outcome
- grain weighte0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suppressed expression of miR167 (STTM/MIM167) substantially increased grain weight”
t0“rice”
Claim · model-written summary
Field yield increased by 12.90%-21.94% with enhanced grain filling.
- Taxon
- riceOryza sativat0
- Treatment
- STTM/MIM167e0
- Outcome
- yielde0
- Direction
- increasede0
- Value
- 12.90%-21.94%e0
- Setting
- field teste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suppressed expression of miR167 (STTM/MIM167) substantially increased grain weight. In a field test, the yield increased up to 12.90%-21.94% because of a significantly enhanced grain filling rate”
t0“rice”
Claim · model-written summary
Grain filling rate was significantly enhanced in the field test.
- Taxon
- riceOryza sativat0
- Treatment
- STTM/MIM167e0
- Outcome
- grain filling ratee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suppressed expression of miR167 (STTM/MIM167) substantially increased grain weight. In a field test, the yield increased up to 12.90%-21.94% because of a significantly enhanced grain filling rate”
t0“rice”
Claim · model-written summary
miR159 regulates miR167 expression.
- Taxon
- riceOryza sativat0
- Gene
- miR159e0
- Outcome
- miR167 expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“biochemical and genetic analyses revealed the regulatory effects of miR159 on miR167 expression”
t0“rice”
Claim · model-written summary
OsARF12 mediates miR167 regulation of rice grain filling.
- Taxon
- riceOryza sativae0
- Gene
- OsARF12e0
- Outcome
- rice grain fillinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsARF12 is the major mediator by which miR167 regulates rice grain filling”
Claim · model-written summary
OsARF12 overexpression produced grain phenotypes resembling STTM/MIM167.
- Taxon
- riceOryza sativat0
- Treatment
- Overexpression of OsARF12e0
- Gene
- OsARF12e0
- Outcome
- grain weight and grain filling phenotypese0
- Direction
- resemblinge0
- Comparator
- STTM/MIM167 plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of OsARF12 produced grain weight and grain filling phenotypes resembling those of STTM/MIM167 plants”
t0“rice”
Claim · model-written summary
OsARF12 directly activates OsCDKF;2 expression through a promoter motif.
- Taxon
- riceOryza sativat0
- Gene
- OsARF12e0
- Outcome
- OsCDKF;2 expressione0
- Qualifier
- directly binding to the TGTCGG motife0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsARF12 activates OsCDKF;2 expression by directly binding to the TGTCGG motif in its promoter region”
t0“rice”
Claim · model-written summary
OsARF12 positively regulates grain filling and size by targeting OsCDKF;2.
- Taxon
- riceOryza sativat0
- Gene
- OsARF12e0
- Outcome
- grain filling and grain sizee0
- Qualifier
- targeting OsCDKF;2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsARF12 positively regulates grain filling and grain size by targeting OsCDKF;2”
t0“rice”
Claim · model-written summary
RNA sequencing linked the module to cell development and hormone pathways.
- Taxon
- riceOryza sativat0
- Gene
- miR167-OsARF12 modulee0
- Outcome
- cell development process and hormone pathwayse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the miR167-OsARF12 module is involved in the cell development process and hormone pathways”
t0“rice”
Claim · model-written summary
OsARF12 overexpression enhanced sensitivity to exogenous auxin and brassinosteroid.
- Taxon
- riceOryza sativat0
- Material
- OsARF12-overexpressing plantse0
- Treatment
- exogenous auxin and brassinosteroid (BR) treatmente0
- Outcome
- sensitivitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsARF12-overexpressing plants and cdkf;2 mutants exhibited enhanced and reduced sensitivity to exogenous auxin and brassinosteroid (BR) treatment”
t0“rice”
Claim · model-written summary
cdkf;2 mutants showed reduced sensitivity to exogenous auxin and brassinosteroid.
- Taxon
- riceOryza sativat0
- Material
- cdkf;2 mutantse0
- Treatment
- exogenous auxin and brassinosteroid (BR) treatmente0
- Outcome
- sensitivitye0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsARF12-overexpressing plants and cdkf;2 mutants exhibited enhanced and reduced sensitivity to exogenous auxin and brassinosteroid (BR) treatment”
t0“rice”
The same questions in other papers
transgenic · yield · Oryza sativa (rice)Positive and negative results
80 papers in the same direction as this paper · 18 in the opposite direction · 3 reporting no difference from an alternative
- no effect
- negative
- negative
98 papers more in this group
See all 102 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.