Abstract
Domain of Unknown Function 231-containing proteins (DUF231) are plant specific and their function is largely unknown. Studies in the model plants Arabidopsis and rice suggested that some DUF231 proteins act in the process of O -acetyl substitution of hemicellulose and esterification of pectin. However, little is known about the function of DUF231 proteins in woody plant species. This study provides evidence supporting that one member of DUF231 family proteins in the woody perennial plant Populus deltoides (genotype WV94), PdDUF231A, has a role in the acetylation of xylan and affects cellulose biosynthesis. A total of 52 DUF231-containing proteins were identified in the Populus genome. In P. deltoides transgenic lines overexpressing PdDUF231A ( OXPdDUF231A ), glucose and cellulose contents were increased. Consistent with these results, the transcript levels of cellulose biosynthesis-related genes were increased in the OXPdDUF231A transgenic lines. Furthermore, the relative content of total acetylated xylan was increased in the OXPdDUF231A transgenic lines. Enzymatic saccharification assays revealed that the rate of glucose release increased in OXPdDUF231A transgenic lines. Plant biomass productivity was also increased in OXPdDUF231A transgenic lines. These results suggest that PdDUF231A affects cellulose biosynthesis and plays a role in the acetylation of xylan. PdDUF231A is a promising target for genetic modification for biofuel production because biomass productivity and compositional quality can be simultaneously improved through overexpression.
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Claims 7
Claim · model-written summary
The Populus genome contains 52 DUF231-containing proteins.
PopulusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 52 DUF231-containing proteins were identified in the Populus genome.”
t0“Populus”
Claim · model-written summary
PdDUF231A overexpression increased glucose and cellulose contents in P. deltoides.
- Material
- OXPdDUF231Ae0
- Treatment
- overexpressing PdDUF231Ae0
- Gene
- PdDUF231Ae0
- Outcome
- glucose and cellulose contentse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In P. deltoides transgenic lines overexpressing PdDUF231A ( OXPdDUF231A ), glucose and cellulose contents were increased.”
t0“Populus”
Claim · model-written summary
Cellulose biosynthesis-related gene transcript levels increased in OXPdDUF231A lines.
- Material
- OXPdDUF231Ae0
- Treatment
- OXPdDUF231A transgenic linese0
- Outcome
- transcript levels of cellulose biosynthesis-related genese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transcript levels of cellulose biosynthesis-related genes were increased in the OXPdDUF231A transgenic lines.”
t0“Populus”
Claim · model-written summary
Total acetylated xylan content increased in OXPdDUF231A lines.
- Material
- OXPdDUF231Ae0
- Treatment
- OXPdDUF231A transgenic linese0
- Outcome
- relative content of total acetylated xylane0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the relative content of total acetylated xylan was increased in the OXPdDUF231A transgenic lines.”
t0“Populus”
Claim · model-written summary
OXPdDUF231A lines had an increased rate of glucose release in saccharification assays.
- Material
- OXPdDUF231Ae0
- Treatment
- OXPdDUF231A transgenic linese0
- Outcome
- rate of glucose releasee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the rate of glucose release increased in OXPdDUF231A transgenic lines.”
t0“Populus”
Claim · model-written summary
Plant biomass productivity increased in OXPdDUF231A lines.
- Material
- OXPdDUF231Ae0
- Treatment
- OXPdDUF231A transgenic linese0
- Outcome
- Plant biomass productivitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plant biomass productivity was also increased in OXPdDUF231A transgenic lines.”
t0“Populus”
Claim · model-written summary
The authors propose PdDUF231A as a genetic modification target for biofuel production.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PdDUF231A is a promising target for genetic modification for biofuel production because biomass productivity and compositional quality can be simultaneously improved through overexpression.”
t0“Populus”
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