Abstract
The initial reaction in the pathway leading to the production of indole-3-acetic acid (IAA) in plants is the reaction between chorismate and glutamine to produce anthranilate, catalysed by the enzyme anthranilate synthase (ASA; EC 4.1.3.27). Compared with non-transgenic controls, leaves of transgenic poplar with ectopic expression of the pine cytosolic glutamine synthetase (GS1a; EC 6.3.1.2) produced significantly greater glutamine and significantly enhanced ASA α-subunit (ASA1) transcript and protein (approximately 130% and 120% higher than in the untransformed controls, respectively). Similarly, tobacco leaves fed with 30 mM glutamine and 2 mM chorismate showed enhanced ASA1 transcript and protein (175% and 90% higher than controls, respectively). Furthermore, free IAA was significantly elevated both in leaves of GS1a transgenic poplar and in tobacco leaves fed with 30 mM glutamine and 2 mM chorismate. These results indicated that enhanced cellular glutamine may account for the enhanced growth in GS transgenic poplars through the regulation of auxin biosynthesis.
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Claims 8
Claim · model-written summary
Transgenic poplar leaves had significantly greater glutamine than controls.
- Organ / stage
- leavese0
- Treatment
- ectopic expression of the pine cytosolic glutamine synthetasee0
- Gene
- pine cytosolic glutamine synthetasee0
- Outcome
- glutaminee0
- Direction
- greatere0
- Comparator
- non-transgenic controlse0
PopulusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with non-transgenic controls, leaves of transgenic poplar with ectopic expression of the pine cytosolic glutamine synthetase (GS1a; EC 6.3.1.2) produced significantly greater glutamine”
Claim · model-written summary
Poplar ASA1 transcript abundance was about 130% higher than controls.
- Treatment
- transgenict0
- Gene
- ASA1e0
- Outcome
- ASA α-subunit (ASA1) transcripte0
- Direction
- highere0
- Value
- approximately 130%e0
- Comparator
- untransformed controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“poplar transgenic”
e0“ASA α-subunit (ASA1) transcript and protein (approximately 130% and 120% higher than in the untransformed controls, respectively)”
Claim · model-written summary
Poplar ASA1 protein abundance was about 120% higher than controls.
- Treatment
- transgenict0
- Gene
- ASA1e0
- Outcome
- proteine0
- Direction
- highere0
- Value
- 120%e0
- Comparator
- untransformed controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“poplar transgenic”
e0“ASA α-subunit (ASA1) transcript and protein (approximately 130% and 120% higher than in the untransformed controls, respectively)”
Claim · model-written summary
Glutamine-fed tobacco had 175% higher ASA1 transcript abundance.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- 30 mM glutamine and 2 mM chorismatee0
- Gene
- ASA1e0
- Outcome
- ASA1 transcripte0
- Direction
- highere0
- Value
- 175%e0
- Comparator
- controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco leaves fed with 30 mM glutamine and 2 mM chorismate showed enhanced ASA1 transcript and protein (175% and 90% higher than controls, respectively)”
Claim · model-written summary
Glutamine-fed tobacco had 90% higher ASA1 protein abundance.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- 30 mM glutamine and 2 mM chorismatee0
- Gene
- ASA1e0
- Outcome
- proteine0
- Direction
- highere0
- Value
- 90%e0
- Comparator
- controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco leaves fed with 30 mM glutamine and 2 mM chorismate showed enhanced ASA1 transcript and protein (175% and 90% higher than controls, respectively)”
Claim · model-written summary
Free IAA was significantly elevated in GS1a poplar leaves.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“free IAA was significantly elevated both in leaves of GS1a transgenic poplar”
Claim · model-written summary
Free IAA was significantly elevated in glutamine and chorismate fed tobacco leaves.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- fed with 30 mM glutamine and 2 mM chorismatee0
- Outcome
- free IAAe0
- Direction
- elevatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“free IAA was significantly elevated both in leaves of GS1a transgenic poplar and in tobacco leaves fed with 30 mM glutamine and 2 mM chorismate”
Claim · model-written summary
The authors propose elevated glutamine regulates auxin biosynthesis and enhanced poplar growth.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enhanced cellular glutamine may account for the enhanced growth in GS transgenic poplars through the regulation of auxin biosynthesis”
The same questions in other papers
transgenic · molecular: expression · Populus (poplar)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
transgenic · molecular: metabolites · Populus (poplar)Results agree
5 papers in the same direction as this paper
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.