Abstract
Poplars overexpressing a bacterial Γ ‐glutamylcysteine synthetase ( Γ ‐ECS) in the cytosol (lines ggs11 and ggs28) had a 30‐fold increase in foliar Γ ‐ECS activity relative to untransformed controls. Foliar Γ ‐glutamylcysteine ( Γ ‐EC) was increased by 10‐fold while foliar glutathione accumulation increased by up to 3.5‐fold in the leaves of the transformants. Untransformed and transformed poplars were grown with different soil concentrations of cadmium (0–1100 μg g −1 soil) for 2 weeks. Cadmium accumulated in the leaves of both transformed and untransformed poplars and growth was inhibited. Growth inhibition and foliar cadmium accumulation were greatest at the highest soil cadmium concentrations in all lines. Exposure to cadmium enhanced the foliar cysteine, Γ ‐EC and glutathione pools in all lines but less glutathione was present in the leaves of the untransformed controls than the transformants under all growth conditions. Cadmium‐induced changes in the activities of malic enzyme, isocitrate dehydrogenase and guaiacol peroxidase were less pronounced in the leaves of the transformed poplars overexpressing Γ ‐ECS than in the untransformed controls. Glutamate dehydrogenase and glutathione reductase activities were unchanged by exposure to cadmium. We conclude that overexpression of Γ ‐ECS activity and foliar glutathione accumulation in transformed poplar allows greater tissue cadmium accumulation but has only a marginal effect on cadmium tolerance in poplar.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 13
Claim · model-written summary
Transformed poplars had 30-fold higher foliar Γ ‐ECS activity than untransformed controls.
- Material
- lines ggs11 and ggs28e0
- Organ / stage
- foliare0
- Treatment
- overexpressing a bacterial Γ ‐glutamylcysteine synthetasee0
- Gene
- Γ ‐ECSe0
- Outcome
- foliar Γ ‐ECS activitye0
- Direction
- increasee0
- Value
- 30‐folde0
- Comparator
- untransformed controlse0
PopulusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Poplars overexpressing a bacterial Γ ‐glutamylcysteine synthetase ( Γ ‐ECS) in the cytosol (lines ggs11 and ggs28) had a 30‐fold increase in foliar Γ ‐ECS activity relative to untransformed controls”
Claim · model-written summary
Foliar Γ ‐EC increased tenfold in transformants.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Foliar Γ ‐glutamylcysteine ( Γ ‐EC) was increased by 10‐fold while foliar glutathione accumulation increased by up to 3.5‐fold in the leaves of the transformants”
t0“poplars”
Claim · model-written summary
Foliar glutathione accumulation increased by up to 3.5-fold in transformants.
- Treatment
- transformantse0
- Outcome
- foliar glutathione accumulatione0
- Direction
- increasede0
- Value
- up to 3.5‐folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“foliar glutathione accumulation increased by up to 3.5‐fold in the leaves of the transformants”
t0“poplars”
Claim · model-written summary
Cadmium accumulated in leaves of transformed and untransformed poplars.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cadmium accumulated in the leaves of both transformed and untransformed poplars”
Claim · model-written summary
Cadmium exposure inhibited poplar growth.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cadmium accumulated in the leaves of both transformed and untransformed poplars and growth was inhibited”
Claim · model-written summary
Growth inhibition was greatest at the highest soil cadmium concentrations in all lines.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Growth inhibition and foliar cadmium accumulation were greatest at the highest soil cadmium concentrations in all lines”
t0“poplars”
Claim · model-written summary
Foliar cadmium accumulation was greatest at the highest soil cadmium concentrations in all lines.
- Treatment
- highest soil cadmium concentrationse0
- Outcome
- foliar cadmium accumulatione0
- Direction
- greateste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Growth inhibition and foliar cadmium accumulation were greatest at the highest soil cadmium concentrations in all lines”
t0“poplars”
Claim · model-written summary
Cadmium exposure enhanced foliar cysteine, Γ ‐EC and glutathione pools in all lines.
- Treatment
- Exposure to cadmiume0
- Outcome
- foliar cysteine, Γ ‐EC and glutathione poolse0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exposure to cadmium enhanced the foliar cysteine, Γ ‐EC and glutathione pools in all lines”
t0“poplars”
Claim · model-written summary
Untransformed controls had less leaf glutathione than transformants under all growth conditions.
- Material
- untransformed controlse0
- Organ / stage
- leavese0
- Outcome
- glutathionee0
- Direction
- lesse0
- Qualifier
- under all growth conditionse0
- Comparator
- transformantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“less glutathione was present in the leaves of the untransformed controls than the transformants under all growth conditions”
t0“poplars”
Claim · model-written summary
Cadmium-induced changes in malic enzyme, isocitrate dehydrogenase and guaiacol peroxidase activities were less pronounced in transformed leaves than controls.
- Material
- transformed poplarse0
- Gene
- malic enzyme, isocitrate dehydrogenase and guaiacol peroxidasee0
- Outcome
- changes in the activitiese0
- Direction
- less pronouncede0
- Comparator
- untransformed controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cadmium‐induced changes in the activities of malic enzyme, isocitrate dehydrogenase and guaiacol peroxidase were less pronounced in the leaves of the transformed poplars overexpressing Γ ‐ECS than in the untransformed controls”
Claim · model-written summary
Glutamate dehydrogenase and glutathione reductase activities were unchanged by cadmium exposure.
- Treatment
- exposure to cadmiume0
- Gene
- Glutamate dehydrogenase and glutathione reductasee0
- Outcome
- Glutamate dehydrogenase and glutathione reductase activitiese0
- Direction
- unchangede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Glutamate dehydrogenase and glutathione reductase activities were unchanged by exposure to cadmium”
t0“poplars”
Claim · model-written summary
The authors conclude Γ ‐ECS overexpression permits greater tissue cadmium accumulation.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of Γ ‐ECS activity and foliar glutathione accumulation in transformed poplar allows greater tissue cadmium accumulation”
Claim · model-written summary
The authors conclude transformed poplar has only marginally improved cadmium tolerance.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of Γ ‐ECS activity and foliar glutathione accumulation in transformed poplar allows greater tissue cadmium accumulation but has only a marginal effect on cadmium tolerance in poplar”
The same questions in other papers
cadmium atom · growth · Populus (poplar)Results differ
8 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
7 papers more in this group
See all 11 papers →cadmium atom · molecular: metabolites · Populus (poplar)Positive and negative results
2 in the opposite direction
- negative
- no effect
overexpression · molecular: metabolites · Populus (poplar)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- negative
- no effect
- positive
transgenic · molecular: metabolites · Populus (poplar)Results agree
5 papers in the same direction as this paper
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.