Abstract
Abstract In Populus , the transcripts of fasciclin-like arabinogalactan proteins (FLAs) are accumulated in tension wood (TW) xylem, however their biological functions in TW formation are largely unknown. In this work, we demonstrated that PtFLA6, one of poplar TW-associated PtFLAs, was abundantly expressed in TW, and mainly localized in differentiating G-fibers. The bended stems of PtFLA6 antisense transgenic poplar showed decreased transcripts of PtFLAs , including PtFLA6 , and reduced PtFLA6 like proteins, leading to inhibited TW differentiation and formation. We also showed that gibberellin A3 (GA 3 ) was enriched in the xylem of TW side, accompanied with a lowered level of PtRGA1, a poplar DELLA protein. When GA 3 biosynthesis was restrained in the bended poplar stems by a GA biosynthesis inhibitor (daminozide), TW formation was obviously repressed, as a result of restricted PtRGA1 degradation, and reduced PtFLA6 like proteins and PtFLA expression. Further studies indicated that PtFLAs were negatively regulated by PtRGA1. This study suggests that PtFLAs play important roles in the poplar TW formation, possibly regulated by GA signaling.
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Claims 9
Claim · model-written summary
PtFLA6 was abundant in tension wood and localized mainly in differentiating G-fibers.
PopulusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PtFLA6, one of poplar TW-associated PtFLAs, was abundantly expressed in TW, and mainly localized in differentiating G-fibers”
e1“In Populus”
Claim · model-written summary
PtFLA6 antisense poplar had lower PtFLA transcripts and PtFLA6-like proteins.
- Treatment
- PtFLA6 antisense transgenice0
- Gene
- PtFLA6e0
- Outcome
- transcripts of PtFLAs , including PtFLA6 , and reduced PtFLA6 like proteinse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The bended stems of PtFLA6 antisense transgenic poplar showed decreased transcripts of PtFLAs , including PtFLA6 , and reduced PtFLA6 like proteins”
e1“In Populus”
Claim · model-written summary
PtFLA6 antisense inhibited tension wood differentiation and formation.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The bended stems of PtFLA6 antisense transgenic poplar showed decreased transcripts of PtFLAs , including PtFLA6 , and reduced PtFLA6 like proteins, leading to inhibited TW differentiation and formation”
e1“In Populus”
Claim · model-written summary
GA3 was enriched on the tension wood side of xylem.
- Outcome
- gibberellin A3 (GA 3 ) was enriched in the xylem of TW sidee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“gibberellin A3 (GA 3 ) was enriched in the xylem of TW side”
e1“In Populus”
Claim · model-written summary
PtRGA1 was lower on the tension wood side.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a lowered level of PtRGA1, a poplar DELLA protein”
e1“In Populus”
Claim · model-written summary
Daminozide repression of GA biosynthesis repressed tension wood formation.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When GA 3 biosynthesis was restrained in the bended poplar stems by a GA biosynthesis inhibitor (daminozide), TW formation was obviously repressed”
e1“In Populus”
Claim · model-written summary
Daminozide restricted PtRGA1 degradation and reduced PtFLA6-like proteins and PtFLA expression.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“as a result of restricted PtRGA1 degradation, and reduced PtFLA6 like proteins and PtFLA expression”
e1“GA biosynthesis inhibitor (daminozide)”
e2“In Populus”
Claim · model-written summary
PtRGA1 negatively regulated PtFLAs.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PtFLAs were negatively regulated by PtRGA1”
e1“In Populus”
Claim · model-written summary
The authors infer PtFLAs participate in tension wood formation, possibly through GA signaling.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PtFLAs play important roles in the poplar TW formation, possibly regulated by GA signaling”
e1“In Populus”
The same questions in other papers
transgenic · molecular: expression · Populus (poplar)Positive and negative results
2 in the opposite direction
- positive
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loss of function · molecular: expression · Populus (poplar)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.