Abstract
Among the most common Musaceae in Venezuela are the plantain ‘Giant Harton’ (Musa AAB), banana (Musa AAA) subgroup ‘Cavendish’, ‘Cambur Manzano’ (‘Apple Banana’) (Musa AAB) and ‘Topocho Criollo’ (‘Bluggoe Banana’) (Musa ABB). The objective of this study was to generate plantlets of ‘Cambur Manzano’, ‘Topocho Criollo’ and ‘Grand Nain’ (Cavendish subgroup), fruits of economic importance in Venezuela. Micropropagation was conducted in two phases: initiation and multiplication. In the initiation phase, caulinary apexes of 5 to 8 mm long were cultivated in liquid culture media with Heller bridge support (M1) and in semi-solid (M2) media. The liquid culture means offered better results, since a survival of 73.3% and a lower percentage of necrosis (0.42%) was obtained. By contrast, using the semi-solid medium, the lowest values of survival (43.7) and increased necrosis explants (27.7) were recorded. Banana cultivars ‘Manzano’, ‘Topocho Criollo’ and ‘Grand Nain’ had similar percentages of survival, necrosis and pollution in the initiation phase. In the multiplication phase, explants consisting outbreaks 3 cm in length were taken. To evaluate the influence of the sub-culture of explants in the number of shoots, it was observed that sub-cultures had no influence on the number of outbreaks of ‘Manzano’ and ‘Topocho Criollo’. However, in ‘Grand Nain’, a trend was observed toward their increase by rising the subcultures. In the multiplication phase, cultivars and subcultures did not affect survival, necrosis and pollution rates.
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Claims 9
Claim · model-written summary
Liquid medium gave 73.3% survival.
- Treatment
- liquid culture media with Heller bridge support (M1)e1
- Outcome
- survivale0
- Direction
- bettere0
- Value
- 73.3%e0
- Comparator
- semi-solid (M2) mediae1
MusaOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The liquid culture means offered better results, since a survival of 73.3%”
t0“tres variedades de Musa spp.”
e1“liquid culture media with Heller bridge support (M1) and in semi-solid (M2) media”
Claim · model-written summary
Liquid medium yielded 0.42% necrosis.
- Treatment
- liquid culture media with Heller bridge support (M1)e1
- Outcome
- necrosise0
- Direction
- lowere0
- Value
- 0.42%e0
- Comparator
- semi-solid (M2) mediae1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a lower percentage of necrosis (0.42%)”
t0“tres variedades de Musa spp.”
e1“liquid culture media with Heller bridge support (M1) and in semi-solid (M2) media”
Claim · model-written summary
Semi-solid medium gave the lowest survival value, 43.7.
- Treatment
- semi-solid mediume0
- Outcome
- survivale0
- Direction
- loweste0
- Value
- 43.7e0
- Comparator
- liquid culture media with Heller bridge support (M1)e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“using the semi-solid medium, the lowest values of survival (43.7)”
t0“tres variedades de Musa spp.”
e1“liquid culture media with Heller bridge support (M1)”
Claim · model-written summary
Semi-solid medium increased explant necrosis to 27.7.
- Treatment
- semi-solid mediume1
- Outcome
- necrosis explantse0
- Direction
- increasede0
- Value
- 27.7e0
- Comparator
- liquid culture media with Heller bridge support (M1)e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased necrosis explants (27.7)”
t0“tres variedades de Musa spp.”
e1“using the semi-solid medium”
e2“liquid culture media with Heller bridge support (M1)”
Claim · model-written summary
The three cultivars had similar initiation survival, necrosis and pollution.
Verbatim
e0“Banana cultivars ‘Manzano’, ‘Topocho Criollo’ and ‘Grand Nain’ had similar percentages of survival, necrosis and pollution in the initiation phase”
Claim · model-written summary
Subcultures did not influence shoot number in Manzano.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sub-cultures had no influence on the number of outbreaks of ‘Manzano’ and ‘Topocho Criollo’”
t0“tres variedades de Musa spp.”
Claim · model-written summary
Subcultures did not influence shoot number in Topocho Criollo.
- Material
- ‘Topocho Criollo’e0
- Treatment
- sub-culturese0
- Outcome
- number of outbreakse0
- Direction
- no influencee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sub-cultures had no influence on the number of outbreaks of ‘Manzano’ and ‘Topocho Criollo’”
t0“tres variedades de Musa spp.”
Claim · model-written summary
Shoot number in Grand Nain tended to increase with more subcultures.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in ‘Grand Nain’, a trend was observed toward their increase by rising the subcultures”
e1“number of shoots”
t0“tres variedades de Musa spp.”
Claim · model-written summary
Cultivars and subcultures did not affect multiplication survival, necrosis or pollution.
- Organ / stage
- multiplication phasee0
- Treatment
- cultivars and subculturese0
- Outcome
- survival, necrosis and pollution ratese0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the multiplication phase, cultivars and subcultures did not affect survival, necrosis and pollution rates”
t0“tres variedades de Musa spp.”
The same questions in other papers
in vitro · disease · MusaPositive and negative results
3 papers in the same direction as this paper · 4 in the opposite direction
- no effect
- negative
- no effect
4 papers more in this group
See all 8 papers →in vitro · survival · MusaResults agree
4 papers in the same direction as this paper
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.