Paper · 2023 · International Journal of Environment and Climate Change

Enhancing Gerbera Micropropagation Efficiency and Genetic Fidelity through Cytokinin and Auxin Combination Strategies

Satish Kumar; S. K. Sehrawat; D. S. Dahiya; et al.

5
claims
11
verbatim quotes
1
citations
3
research areas
✓
Open access

Abstract

The present investigation was carried out to assess the impact of 6-benzyladenine (BA) in conjunction with Indole-3-butyric acid (IBA) on gerbera explant establishment under micropropagation. Additionally, the effects of BA, either individually or in combination with Kinetin (KIN), on shoot proliferation in two Gerbera cultivars, namely Kormoran and Dolores was experimented too. Throughout the experiment, various morphological changes were documented occurring during these micropropagation phases and also monitored potential genetic alterations using SSR markers. The studies revealed that the combination of BA and IBA yielded exceptional results, achieving a 100% success rate in explant regeneration within the shortest time frame. Notably, when BA and IBA were applied at lower concentrations, the number of shoots generated was reduced. However, the most substantial proliferation of shoots was observed when the growth medium contained 4 mg of BA and 0.5 mg of IBA per litre. Furthermore, our investigation into genetic fidelity using SSR analysis revealed no detectable polymorphism between the mother plant and the micropropagated plantlets in both the Gerbera cultivars, affirming the reliability of the micropropagation method in preserving genetic consistency.

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Claims 5

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

BA combined with IBA gave a 100% explant regeneration success rate in Gerbera within the shortest time frame.

Taxon
GerberaGerberae0
Treatment
combination of BA and IBAe1
Outcome
explant regeneratione1
Direction
achievinge1
Value
100%e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Gerbera
Gerbera · Taxon · picture of the genus (one of its species)Novita Saulina Sitohang · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“shoot proliferation in two Gerbera cultivars, namely Kormoran and Dolores”

✓ located verbatim in the source · abstract, characters 286–359 · show in text ↑
e1

“the combination of BA and IBA yielded exceptional results, achieving a 100% success rate in explant regeneration within the shortest time frame”

✓ located verbatim in the source · abstract, characters 596–739 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

At lower BA and IBA concentrations, the number of shoots in Gerbera was reduced.

Taxon
GerberaGerberae0
Treatment
lower concentrationse1
Outcome
number of shootse1
Direction
reducede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Gerbera
Gerbera · Taxon · picture of the genus (one of its species)Novita Saulina Sitohang · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“shoot proliferation in two Gerbera cultivars, namely Kormoran and Dolores”

✓ located verbatim in the source · abstract, characters 286–359 · show in text ↑
e1

“when BA and IBA were applied at lower concentrations, the number of shoots generated was reduced”

✓ located verbatim in the source · abstract, characters 750–846 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The most substantial shoot proliferation in Gerbera occurred on medium with 4 mg BA and 0.5 mg IBA per litre.

Taxon
GerberaGerberae0
Treatment
4 mg of BA and 0.5 mg of IBA per litree1
Outcome
proliferation of shootse1
Direction
most substantiale1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Gerbera
Gerbera · Taxon · picture of the genus (one of its species)Novita Saulina Sitohang · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“shoot proliferation in two Gerbera cultivars, namely Kormoran and Dolores”

✓ located verbatim in the source · abstract, characters 286–359 · show in text ↑
e1

“the most substantial proliferation of shoots was observed when the growth medium contained 4 mg of BA and 0.5 mg of IBA per litre”

✓ located verbatim in the source · abstract, characters 857–986 · show in text ↑
Observationobservedoriginal resultno effectmeasured

Claim · model-written summary

No detectable polymorphism was found between mother plants and micropropagated plantlets in both Gerbera cultivars.

Taxon
GerberaGerberae0
Outcome
polymorphisme1
Comparator
mother plante1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Gerbera
Gerbera · Taxon · picture of the genus (one of its species)Novita Saulina Sitohang · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“shoot proliferation in two Gerbera cultivars, namely Kormoran and Dolores”

✓ located verbatim in the source · abstract, characters 286–359 · show in text ↑
e1

“revealed no detectable polymorphism between the mother plant and the micropropagated plantlets in both the Gerbera cultivars”

✓ located verbatim in the source · abstract, characters 1060–1184 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

The micropropagation method preserved genetic consistency relative to the mother plant in Gerbera.

Taxon
GerberaGerberae0
Outcome
genetic consistencye2
Comparator
mother plante1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Gerbera
Gerbera · Taxon · picture of the genus (one of its species)Novita Saulina Sitohang · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“shoot proliferation in two Gerbera cultivars, namely Kormoran and Dolores”

✓ located verbatim in the source · abstract, characters 286–359 · show in text ↑
e1

“revealed no detectable polymorphism between the mother plant and the micropropagated plantlets in both the Gerbera cultivars”

✓ located verbatim in the source · abstract, characters 1060–1184 · show in text ↑
e2

“affirming the reliability of the micropropagation method in preserving genetic consistency”

✓ located verbatim in the source · abstract, characters 1186–1276 · show in text ↑

indole-3-butyric acid · growth · GerberaResults agree

5 papers: 5 positive, 0 negative or no effect

4 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

N-benzyladenine · growth · GerberaResults agree

16 papers: 16 positive, 0 negative or no effect

15 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

12 papers more in this group

See all 16 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.