Abstract
Abiotic and biotic environmental factors, such as drought, salinity, extreme temperatures, pollution and pathogen attack seriously limit agricultural productivity and disturb natural environment. Most stress conditions may increase production of reactive oxygen species (ROS) and lead to unspecific oxidation of proteins, membrane lipids and nucleic acids. Salt stress tolerance depends on activation of cellular responses, such as accumulation of compatible solutes and induction of antioxidative system which can prevent cell damage. The objective of this research was to investigate the effects of 100 mM and 200 mM NaCl on in vitro grown dihaploide lines of Nicotiana tabacum L. Proline content, lipid peroxidation level and activities of antioxidative enzymes important in ROS elimination - guaiacol peroxidase (POD), ascorbat peroxidase (APX), catalase (CAT) and superoxide dismutase (SOD), were evaluated. Dihaploides were obtained from midvein explants of haploids derived from anthers of the hybrid DH10, a cross of the line GV3 and cv. Virginia D (VaD). Dihaploid lines used in this experiment (207DH, 238DH, 239DH, 244DH) as well as DH10, GV3 and VaD were previously tested for tolerance to potato virus Y (PVY)1. Most of the plants treated with NaCl, especially GV3, DH10, 207DH and 238DH showed proline accumulation, which followed the increase of applied salt concentrations. In most of the cases, treatments did not cause significant changes of lipid peroxidation level. Exceptions were lines 207DH and 244DH where lipid peroxidation level decreased in plants treated with higher concentration of NaCl comparing to corresponding controls. Generally, POD activity was significantly higher in GV3 and VaD, even in control plants. Salt treatment did not change POD activity in most of the lines, except in 244DH. Similar results were obtained with APX activity. The most pronounced CAT activity was noticed in GV3 and 239DH after treatment with 100 mM NaCl where new isoenzyme was detected. Remarkably increased SOD activity was noticed in line 239DH after treatment with 200 mM NaCl. In conclusion, the significant proline accumulation accompanied with increase of antioxidative enzymes activity in GV3 and pronounced induction of antioxidative enzymes in GV3, VaD and 239DH suggest that these tobacco lines have prominent response to salinity stress. Besides well-known role in osmotic adjustment, accumulated proline could also contribute to protection of membrane integrity and ROS elimination2, which could explain why salinity did not cause increased level of lipid peroxidation in tested tobacco lines. In our ongoing research additional parameters will be considered to evaluate salinity tolerance of selected tobacco lines. 1. Smalcelj B, Curkovic-Perica M (2000). Development of anther-derived flue-cured tobacco dihaploids from PVY resistant DH10 hybrid. Austrian J. Agric. Res. 51, 11-17. 2. Szabados L, Savoure A (2009). Proline: a multifunctional amino acid. Trends Plant Sci. 15, 89-97.
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Claims 8
Claim · model-written summary
NaCl increased proline accumulation in most plants, especially GV3, DH10, 207DH and 238DH, with concentration.
- Taxon
- tobacco
Nicotiana tabacumt0 - Material
- GV3, DH10, 207DH and 238DHe0
- Treatment
- NaCle0
- Outcome
- proline accumulatione0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Most of the plants treated with NaCl, especially GV3, DH10, 207DH and 238DH showed proline accumulation, which followed the increase of applied salt concentrations.”
t0“tobacco”
Claim · model-written summary
Most NaCl treatments did not significantly change lipid peroxidation.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- treatmentse0
- Outcome
- lipid peroxidation levele0
- Direction
- did not cause significant changese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In most of the cases, treatments did not cause significant changes of lipid peroxidation level.”
t0“tobacco”
Claim · model-written summary
Higher NaCl concentration decreased lipid peroxidation in 207DH and 244DH versus controls.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- 207DH and 244DHe0
- Treatment
- higher concentration of NaCle0
- Outcome
- lipid peroxidation levele0
- Direction
- decreasede0
- Comparator
- corresponding controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exceptions were lines 207DH and 244DH where lipid peroxidation level decreased in plants treated with higher concentration of NaCl comparing to corresponding controls.”
t0“tobacco”
Claim · model-written summary
POD activity was significantly higher in GV3 and VaD, including controls.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- GV3 and VaDe0
- Outcome
- POD activitye0
- Direction
- highere0
- Qualifier
- even in control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“POD activity was significantly higher in GV3 and VaD, even in control plants.”
t0“tobacco”
Claim · model-written summary
Salt treatment left POD activity unchanged in most lines apart from 244DH.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- Salt treatmente0
- Outcome
- POD activitye0
- Direction
- did not changee0
- Qualifier
- except in 244DHe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Salt treatment did not change POD activity in most of the lines, except in 244DH.”
t0“tobacco”
Claim · model-written summary
APX activity followed a pattern similar to POD.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- APX activitye0
- Qualifier
- Similar resultse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Similar results were obtained with APX activity.”
t0“tobacco”
Claim · model-written summary
CAT activity was most pronounced in GV3 and 239DH after 100 mM NaCl, with a new isoenzyme detected.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- GV3 and 239DHe0
- Treatment
- 100 mM NaCle0
- Outcome
- CAT activitye0
- Direction
- most pronouncede0
- Qualifier
- new isoenzyme was detectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The most pronounced CAT activity was noticed in GV3 and 239DH after treatment with 100 mM NaCl where new isoenzyme was detected.”
t0“tobacco”
Claim · model-written summary
SOD activity rose markedly in 239DH after 200 mM NaCl.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- 239DHe0
- Treatment
- 200 mM NaCle0
- Outcome
- SOD activitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Remarkably increased SOD activity was noticed in line 239DH after treatment with 200 mM NaCl.”
t0“tobacco”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.