Abstract
Majority of injuries observed in plants caused by salinity are associated with the induction of oxidative stress. The accumulation of reactive oxygen species is overwhelmed by the activities of scavenging enzymes and nonenzyme agents, e.g., proline and polyamines that are commonly synthesized directly from ornithine. The present study was undertaken to evaluate the impact of ornithine, as the common source of proline and polyamines, in the biosynthesis of proline and polyamines and in the activation of antioxidant enzymes in the tobacco cells under saline conditions. Six-day-old cells were treated with 50 mM NaCl and 0.5 mM ornithine. These concentrations were selected based on the results of two sets of preliminary studies using different concentrations of ornithine and NaCl. The results demonstrated that the production of proline and putrescine/spermidine was stimulated by ornithine under normal and stress conditions. Ornithine, in the presence or absence of NaCl, significantly increased the antioxidant enzyme activity (up to 325% for catalase, 270% for peroxidase, and 374% for superoxide dismutase), compared to those of the control group. Treatment with ornithine reduced H2O2 to the levels observed in the control cells, while NaCl increased it to 173% of the control. In all treatments, the level of lipid peroxidation was higher than that in the control cells and suppressed the growth of the tobacco cells. On the basis of the results, the treatment of the tobacco cells with ornithine elevates the proline and polyamine contents, with a concurrent decrease in H2O2 content.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
Ornithine stimulated proline production in tobacco cells under normal and saline conditions.
- Taxon
- tobacco
Nicotiana tabacumt0 - Treatment
- ornithinee0
- Outcome
- production of prolinee0
- Direction
- stimulatede0
- Qualifier
- under normal and stress conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results demonstrated that the production of proline and putrescine/spermidine was stimulated by ornithine under normal and stress conditions.”
t0“Ornithine-induced increase of proline and polyamines contents in tobacco cells under salinity conditions”
Claim · model-written summary
Ornithine stimulated putrescine and spermidine production in tobacco cells.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- ornithinee0
- Outcome
- putrescine/spermidinee0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results demonstrated that the production of proline and putrescine/spermidine was stimulated by ornithine under normal and stress conditions.”
t0“Ornithine-induced increase of proline and polyamines contents in tobacco cells under salinity conditions”
Claim · model-written summary
Ornithine significantly increased catalase activity up to 325%.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- Ornithinee0
- Outcome
- catalasee0
- Direction
- increasede0
- Value
- 325%e0
- Qualifier
- in the presence or absence of NaCle0
- Comparator
- control groupe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ornithine, in the presence or absence of NaCl, significantly increased the antioxidant enzyme activity (up to 325% for catalase, 270% for peroxidase, and 374% for superoxide dismutase), compared to those of the control group.”
t0“Ornithine-induced increase of proline and polyamines contents in tobacco cells under salinity conditions”
Claim · model-written summary
Ornithine significantly increased peroxidase activity up to 270%.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- Ornithinee0
- Outcome
- peroxidasee0
- Direction
- increasede0
- Value
- 270%e0
- Qualifier
- in the presence or absence of NaCle0
- Comparator
- control groupe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ornithine, in the presence or absence of NaCl, significantly increased the antioxidant enzyme activity (up to 325% for catalase, 270% for peroxidase, and 374% for superoxide dismutase), compared to those of the control group.”
t0“Ornithine-induced increase of proline and polyamines contents in tobacco cells under salinity conditions”
Claim · model-written summary
Ornithine significantly increased superoxide dismutase activity up to 374%.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- Ornithinee0
- Outcome
- superoxide dismutasee0
- Direction
- increasede0
- Value
- 374%e0
- Qualifier
- in the presence or absence of NaCle0
- Comparator
- control groupe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ornithine, in the presence or absence of NaCl, significantly increased the antioxidant enzyme activity (up to 325% for catalase, 270% for peroxidase, and 374% for superoxide dismutase), compared to those of the control group.”
t0“Ornithine-induced increase of proline and polyamines contents in tobacco cells under salinity conditions”
Claim · model-written summary
Ornithine reduced tobacco cell H2O2 to control levels.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- ornithinee0
- Outcome
- H2O2e0
- Direction
- reducede0
- Comparator
- control cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment with ornithine reduced H2O2 to the levels observed in the control cells, while NaCl increased it to 173% of the control.”
t0“Ornithine-induced increase of proline and polyamines contents in tobacco cells under salinity conditions”
Claim · model-written summary
NaCl increased tobacco cell H2O2 to 173% of control.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- NaCle0
- Outcome
- H2O2e0
- Direction
- increasede0
- Value
- 173%e0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment with ornithine reduced H2O2 to the levels observed in the control cells, while NaCl increased it to 173% of the control.”
t0“Ornithine-induced increase of proline and polyamines contents in tobacco cells under salinity conditions”
Claim · model-written summary
All treatments raised lipid peroxidation above control levels.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- all treatmentse0
- Outcome
- level of lipid peroxidatione0
- Direction
- highere0
- Comparator
- control cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In all treatments, the level of lipid peroxidation was higher than that in the control cells and suppressed the growth of the tobacco cells.”
Claim · model-written summary
All treatments suppressed tobacco cell growth.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- all treatmentse0
- Outcome
- growth of the tobacco cellse0
- Direction
- suppressede0
- Comparator
- control cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In all treatments, the level of lipid peroxidation was higher than that in the control cells and suppressed the growth of the tobacco cells.”
The same questions in other papers
sodium chloride · physiology: oxidative · Nicotiana tabacum (tobacco)Positive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.