Abstract
Methylglyoxal (MG) is one of the aldehydes accumulated in plants under environmental stress. Cytosolic ascorbate peroxidase (cAPX) plays a key role in the protection of cells from oxidative damage by scavenging reactive oxygen species in higher plants. A cDNA encoding cAPX, named NtcAPX, was isolated from Nicotiana tabacum. We characterized recombinant NtcAPX (rNtcAPX) as a fusion protein with glutathione S-transferase to investigate the effects of MG on APX. NtcAPX consists of 250 amino acids and has a deduced molecular mass of 27.5 kDa. The rNtcAPX showed a higher APX activity. MG treatments resulted in a reduction of APX activity and modifications of amino groups in rNtcAPX with increasing K(m) for ascorbate. On the contrary, neither NaCl nor cadmium reduced the activity of APX. The present study suggests that inhibition of APX is in part due to the modification of amino acids by MG.
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Claims 10
Claim · model-written summary
A cDNA encoding NtcAPX was isolated from tobacco.
- Taxon
- Nicotiana tabacum
Nicotiana tabacume0 - Gene
- NtcAPXe0
- Outcome
- cDNA encoding cAPXe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A cDNA encoding cAPX, named NtcAPX, was isolated from Nicotiana tabacum”
Claim · model-written summary
NtcAPX consists of 250 amino acids.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Gene
- NtcAPXe0
- Outcome
- amino acidse0
- Value
- 250 amino acidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NtcAPX consists of 250 amino acids and has a deduced molecular mass of 27.5 kDa”
t0“Nicotiana tabacum”
Claim · model-written summary
NtcAPX has a deduced molecular mass of 27.5 kDa.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Gene
- NtcAPXe0
- Outcome
- deduced molecular masse0
- Value
- 27.5 kDae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NtcAPX consists of 250 amino acids and has a deduced molecular mass of 27.5 kDa”
t0“Nicotiana tabacum”
Claim · model-written summary
Recombinant NtcAPX showed higher APX activity.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Gene
- rNtcAPXe0
- Outcome
- APX activitye0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The rNtcAPX showed a higher APX activity”
t0“Nicotiana tabacum”
Claim · model-written summary
Methylglyoxal treatment reduced activity of recombinant APX.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Treatment
- MGe0
- Gene
- rNtcAPXe0
- Outcome
- APX activitye0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MG treatments resulted in a reduction of APX activity and modifications of amino groups in rNtcAPX with increasing K(m) for ascorbate”
t0“Nicotiana tabacum”
e1“Methylglyoxal (MG)”
Claim · model-written summary
Methylglyoxal modified amino groups in recombinant NtcAPX.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Treatment
- MGe0
- Outcome
- modifications of amino groups in rNtcAPXe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MG treatments resulted in a reduction of APX activity and modifications of amino groups in rNtcAPX with increasing K(m) for ascorbate”
t0“Nicotiana tabacum”
Claim · model-written summary
Methylglyoxal increased the K(m) for ascorbate in recombinant NtcAPX.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Treatment
- MGe0
- Outcome
- K(m) for ascorbatee0
- Direction
- increasinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MG treatments resulted in a reduction of APX activity and modifications of amino groups in rNtcAPX with increasing K(m) for ascorbate”
t0“Nicotiana tabacum”
Claim · model-written summary
NaCl did not reduce APX activity.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Treatment
- NaCle0
- Outcome
- activity of APXe0
- Direction
- neither NaCl nor cadmium reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“neither NaCl nor cadmium reduced the activity of APX”
t0“Nicotiana tabacum”
Claim · model-written summary
Cadmium did not reduce APX activity.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Treatment
- cadmiume0
- Outcome
- activity of APXe0
- Direction
- neither NaCl nor cadmium reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“neither NaCl nor cadmium reduced the activity of APX”
t0“Nicotiana tabacum”
Claim · model-written summary
The authors suggest that methylglyoxal inhibition of APX is partly due to amino acid modification.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Treatment
- MGe0
- Outcome
- inhibition of APXe0
- Qualifier
- modification of amino acidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inhibition of APX is in part due to the modification of amino acids by MG”
t0“Nicotiana tabacum”
The same questions in other papers
cadmium atom · physiology: antioxidant enzymes · Nicotiana tabacum (tobacco)Results differ
4 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- no effect
2 papers more in this group
See all 6 papers →sodium chloride · physiology: antioxidant enzymes · Nicotiana tabacum (tobacco)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.