Abstract
In the present study, we develop an efficient and reproducible in vitro regeneration system for two cultivars viz., Jamila and Tomaland of Solanum lycopersicum L., an economically important vegetable crop throughout the world. Sterilization of seeds with 2.5% (v/v) NaOCl was found to be most effective, about 97% of seeds germinated on cotton in magenta box moistened with sterile half strength (½)Murashige and Skoog (MS) medium. Regeneration efficiency of cotyledonary leaf (CL) and cotyledonary node (CN) explants derived from 08 days old aseptic seedling were assessed on MS medium supplemented with different concentrations of auxins and cytokinin. CL explants were found more responsive in comparison to CN in both the cultivars. Types of basal media were also assessed and found to have a significant effect on shoot regeneration. Highest regeneration frequency and maximum number of shoots were standardized from CL explants on MS medium supplied with 6-benzyl adenine (BA; 5.0 µM), indole-3-butyric acid (IBA; 2.5 µM) and Kinetin (Kin; 10.0 µM). In vitro regenerated microshoots were rooted on ½MS medium containing 0.5 µM indole-3-butyric acid (IBA). Regenerated plantlets with well-developed roots and shoot system were successfully acclimated to ex vitro condition. Genetic uniformity of tissue culture raised plantlets was first time evaluated using flow cytometry and single primer amplification reaction (SPAR) methods viz., DAMD and ISSR. No significant changes in ploidy level and nuclear DNA content profile were observed between in vitro propagated plants and normal plants of both the cultivars. Similarly, the SPAR analysis also revealed monomorphic banding patterns in regenerated plantlets of S. lycopersicum verifying their genetic uniformity and clonal fidelity. This efficient regeneration system can be used as a fast and reproducible method for genetic transformation of this important vegetable crop.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
NaOCl sterilization gave about 97% seed germination.
- Taxon
- Solanum lycopersicum L.
Solanum lycopersicume1 - Organ / stage
- seedse0
- Treatment
- 2.5% (v/v) NaOCle0
- Outcome
- seeds germinatede0
- Direction
- most effectivee0
- Value
- 97%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sterilization of seeds with 2.5% (v/v) NaOCl was found to be most effective, about 97% of seeds germinated on cotton in magenta box moistened with sterile half strength (½)Murashige and Skoog (MS) medium.”
e1“Solanum lycopersicum L.”
Claim · model-written summary
Cotyledonary leaf explants were more responsive than cotyledonary nodes in both cultivars.
- Taxon
- Solanum lycopersicumSolanum lycopersicumt0
- Material
- CL explantse0
- Outcome
- more responsivee0
- Direction
- more responsivee0
- Comparator
- CNe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CL explants were found more responsive in comparison to CN in both the cultivars.”
t0“Solanum lycopersicum”
Claim · model-written summary
Basal media significantly affected shoot regeneration.
- Taxon
- Solanum lycopersicumSolanum lycopersicumt0
- Treatment
- Types of basal mediae0
- Outcome
- shoot regeneratione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Types of basal media were also assessed and found to have a significant effect on shoot regeneration.”
t0“Solanum lycopersicum”
Claim · model-written summary
The specified BA, IBA and Kin medium gave the highest regeneration frequency.
- Taxon
- Solanum lycopersicumSolanum lycopersicumt0
- Organ / stage
- CL explantse0
- Treatment
- MS medium supplied with 6-benzyl adenine (BA; 5.0 µM), indole-3-butyric acid (IBA; 2.5 µM) and Kinetin (Kin; 10.0 µM)e0
- Outcome
- regeneration frequencye0
- Direction
- Higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Highest regeneration frequency and maximum number of shoots were standardized from CL explants on MS medium supplied with 6-benzyl adenine (BA; 5.0 µM), indole-3-butyric acid (IBA; 2.5 µM) and Kinetin (Kin; 10.0 µM).”
t0“Solanum lycopersicum”
Claim · model-written summary
The specified BA, IBA and Kin medium gave the maximum number of shoots.
- Taxon
- Solanum lycopersicumSolanum lycopersicumt0
- Organ / stage
- CL explantse0
- Treatment
- MS medium supplied with 6-benzyl adenine (BA; 5.0 µM), indole-3-butyric acid (IBA; 2.5 µM) and Kinetin (Kin; 10.0 µM)e0
- Outcome
- number of shootse0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Highest regeneration frequency and maximum number of shoots were standardized from CL explants on MS medium supplied with 6-benzyl adenine (BA; 5.0 µM), indole-3-butyric acid (IBA; 2.5 µM) and Kinetin (Kin; 10.0 µM).”
t0“Solanum lycopersicum”
Claim · model-written summary
Regenerated microshoots rooted on half-strength MS with IBA.
- Taxon
- Solanum lycopersicumSolanum lycopersicumt0
- Organ / stage
- microshootse0
- Treatment
- ½MS medium containing 0.5 µM indole-3-butyric acid (IBA)e0
- Outcome
- microshoots were rootede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro regenerated microshoots were rooted on ½MS medium containing 0.5 µM indole-3-butyric acid (IBA).”
t0“Solanum lycopersicum”
Claim · model-written summary
Regenerated plantlets acclimated successfully ex vitro.
- Taxon
- Solanum lycopersicumSolanum lycopersicumt0
- Organ / stage
- plantletse0
- Outcome
- successfully acclimatede0
- Setting
- ex vitro conditione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Regenerated plantlets with well-developed roots and shoot system were successfully acclimated to ex vitro condition.”
t0“Solanum lycopersicum”
Claim · model-written summary
Regenerated plants showed no significant ploidy change versus normal plants.
- Taxon
- Solanum lycopersicumSolanum lycopersicumt0
- Outcome
- ploidy levele0
- Direction
- No significant changese0
- Comparator
- normal plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No significant changes in ploidy level and nuclear DNA content profile were observed between in vitro propagated plants and normal plants of both the cultivars.”
t0“Solanum lycopersicum”
Claim · model-written summary
Regenerated plants showed no significant nuclear DNA content change versus normal plants.
- Taxon
- Solanum lycopersicumSolanum lycopersicumt0
- Outcome
- nuclear DNA content profilee0
- Direction
- No significant changese0
- Comparator
- normal plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No significant changes in ploidy level and nuclear DNA content profile were observed between in vitro propagated plants and normal plants of both the cultivars.”
t0“Solanum lycopersicum”
Claim · model-written summary
SPAR showed monomorphic patterns in regenerated plantlets.
- Taxon
- S. lycopersicumSolanum lycopersicume0
- Outcome
- monomorphic banding patternse0
- Qualifier
- genetic uniformity and clonal fidelitye0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the SPAR analysis also revealed monomorphic banding patterns in regenerated plantlets of S. lycopersicum verifying their genetic uniformity and clonal fidelity.”
The same questions in other papers
N-benzyladenine · growth · Solanum lycopersicum (tomato)Results differ
34 papers in the same direction as this paper · 7 in the opposite direction
- positive
- negative
- negative
38 papers more in this group
See all 42 papers →sodium hypochlorite · growth · Solanum lycopersicum (tomato)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
indole-3-butyric acid · growth · Solanum lycopersicum (tomato)Positive and negative results
7 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
6 papers more in this group
See all 10 papers →kinetin · growth · Solanum lycopersicum (tomato)Positive and negative results
21 papers in the same direction as this paper · 2 in the opposite direction
- negative
- no effect
- positive
20 papers more in this group
See all 24 papers →adenine · growth · Solanum lycopersicum (tomato)Results agree
1 paper in the same direction as this paper
- positive
in vitro · growth · Solanum lycopersicum (tomato)Positive and negative results
92 papers in the same direction as this paper · 25 in the opposite direction · 5 reporting no difference from an alternative
- no effect
- no effect
- no effect
119 papers more in this group
See all 123 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.