Abstract
A series of experiments were conducted to explore the in vitro morphogenic response of tomato genotypes viz., Castle Rock, Punjab Upma, VFN-8 and IPA-3, under different concentrations and combinations of growth regulators. The analysis of variance at 5% level of significance indicated that the differences among different genotypes, hormonal regimes as well as their interactions were statistically significant. The MS medium supplemented with BAP @ 3.0 mg L -1 and IAA 2.5 mg L -1 was optimum for callus induction, plant regeneration and number of shoots per explant. The maximum per cent callus induction and plant regeneration in the genotypes Castle Rock, Punjab Upma, VFN-8 and IPA-3. was 81.23, 76.69, 68.13 and 65.12%; and 46.91, 48.02, 57.14 and 60.23%, respectively, The respective average number of shoots per culture were 7.03, 6.92, 8.19 and 9.19. At higher and lower levels of hormones, a considerable decline was recorded in per cent callus induction, plant regeneration and number of shoots per explant. The best rooting was found to be in the ½ MS medium supplemented with 0.2 mg L -1 IBA. Among the four soil mixtures studied viz., vermiculite, perlite, coco-peat and mixture of three (vermiculite, perlite and cocopeat in the ratio of 1:1:1), maximum plantlet survival rate was recorded in the mixture of three for genotypes Castle Rock and Punjab Upma and in vermiculite for genotype VFN-8.
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Claims 9
Claim · model-written summary
Genotype, hormone regime, and their interaction significantly affected tomato morphogenic response.
- Taxon
- tomato
Solanum lycopersicumt0 - Material
- different genotypese0
- Treatment
- hormonal regimese0
- Outcome
- differencese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“differences among different genotypes, hormonal regimes as well as their interactions were statistically significant”
t0“tomato”
Claim · model-written summary
BAP and IAA supplemented MS medium was optimal for callus induction, regeneration, and shoots per explant.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- MS medium supplemented with BAP @ 3.0 mg L -1 and IAA 2.5 mg L -1e0
- Outcome
- callus induction, plant regeneration and number of shoots per explante0
- Direction
- optimume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The MS medium supplemented with BAP @ 3.0 mg L -1 and IAA 2.5 mg L -1 was optimum for callus induction, plant regeneration and number of shoots per explant”
t0“tomato”
Claim · model-written summary
Callus induction maxima across the four genotypes were 81.23, 76.69, 68.13 and 65.12%.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- Castle Rock, Punjab Upma, VFN-8 and IPA-3e0
- Outcome
- per cent callus inductione0
- Direction
- maximume0
- Value
- 81.23, 76.69, 68.13 and 65.12%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The maximum per cent callus induction and plant regeneration in the genotypes Castle Rock, Punjab Upma, VFN-8 and IPA-3. was 81.23, 76.69, 68.13 and 65.12%; and 46.91, 48.02, 57.14 and 60.23%, respectively”
t0“tomato”
Claim · model-written summary
Regeneration maxima across the four genotypes were 46.91, 48.02, 57.14 and 60.23%.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- Castle Rock, Punjab Upma, VFN-8 and IPA-3e0
- Outcome
- plant regeneratione0
- Direction
- maximume0
- Value
- 46.91, 48.02, 57.14 and 60.23%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The maximum per cent callus induction and plant regeneration in the genotypes Castle Rock, Punjab Upma, VFN-8 and IPA-3. was 81.23, 76.69, 68.13 and 65.12%; and 46.91, 48.02, 57.14 and 60.23%, respectively”
t0“tomato”
Claim · model-written summary
The four genotypes averaged 7.03, 6.92, 8.19 and 9.19 shoots per culture.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- Castle Rock, Punjab Upma, VFN-8 and IPA-3e1
- Outcome
- average number of shoots per culturee0
- Value
- 7.03, 6.92, 8.19 and 9.19e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The respective average number of shoots per culture were 7.03, 6.92, 8.19 and 9.19”
t0“tomato”
e1“the genotypes Castle Rock, Punjab Upma, VFN-8 and IPA-3.”
Claim · model-written summary
Higher and lower hormone levels reduced callus induction, regeneration, and shoots per explant.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- higher and lower levels of hormonese0
- Outcome
- per cent callus induction, plant regeneration and number of shoots per explante0
- Direction
- declinee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At higher and lower levels of hormones, a considerable decline was recorded in per cent callus induction, plant regeneration and number of shoots per explant”
t0“tomato”
Claim · model-written summary
Half-strength MS medium with IBA gave the best rooting.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- ½ MS medium supplemented with 0.2 mg L -1 IBAe0
- Outcome
- rootinge0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The best rooting was found to be in the ½ MS medium supplemented with 0.2 mg L -1 IBA”
t0“tomato”
Claim · model-written summary
The three-component mixture gave maximum survival for Castle Rock and Punjab Upma.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- Castle Rock and Punjab Upmae0
- Treatment
- mixture of threee0
- Outcome
- plantlet survival ratee0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maximum plantlet survival rate was recorded in the mixture of three for genotypes Castle Rock and Punjab Upma”
t0“tomato”
Claim · model-written summary
Vermiculite gave maximum plantlet survival for VFN-8.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- VFN-8e0
- Treatment
- vermiculitee0
- Outcome
- plantlet survival ratee0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maximum plantlet survival rate was recorded in the mixture of three for genotypes Castle Rock and Punjab Upma and in vermiculite for genotype VFN-8”
t0“tomato”
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