Abstract
Ergosterol is the main sterol of most fungi. Production of reactive oxygen species by NADPH oxidase, alkalinization of extracellular space and increase of cytosolic concentration of calcium after the ergosterol treatment on tobacco cells was previously observed as well as the activation of some stress activated mitogen-activated protein kinases on alfalfa cells, even in nano-molar concentrations. Ergosterol activates a signal pathway including phospholipase A2 and protein kinase C. Here, we report about the expression of some defence-related genes after the treatment of tobacco cells and plants with nano-molar concentration of ergosterol. The gene expression of pathogenesis related proteins of families PR1, PR3, PR5 and proteinase inhibitors together with enzymes as phenylalanine ammonia lyase, sesquiterpene cyclase and lipooxygenase was observed by a real-time PCR,. In addition, the concentrations of some phenolic compounds and phytoalexins increased in time. On the other hand, ergosterol did not provoke any leaf necrosis or cell death and the accumulation of PR proteins was slower when compared with another well defined elicitor cryptogein from Phytophthora cryptogea. These data shows that ergosterol is able to activate expression of a number of defence genes and could increase the resistance of plants against the pathogens.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
Ergosterol treatment induced expression of the listed defence genes in tobacco cells and plants.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- nano-molar concentration of ergosterole0
- Gene
- pathogenesis related proteins of families PR1, PR3, PR5e1
- Outcome
- gene expressione1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treatment of tobacco cells and plants with nano-molar concentration of ergosterol”
e1“The gene expression of pathogenesis related proteins of families PR1, PR3, PR5 and proteinase inhibitors together with enzymes as phenylalanine ammonia lyase, sesquiterpene cyclase and lipooxygenase was observed by a real-time PCR,”
Claim · model-written summary
Phenolic compound concentrations increased over time after ergosterol treatment.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- nano-molar concentration of ergosterole0
- Outcome
- concentrations of some phenolic compoundse1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treatment of tobacco cells and plants with nano-molar concentration of ergosterol”
e1“the concentrations of some phenolic compounds and phytoalexins increased in time”
Claim · model-written summary
Phytoalexin concentrations increased over time after ergosterol treatment.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- nano-molar concentration of ergosterole0
- Outcome
- phytoalexinse1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treatment of tobacco cells and plants with nano-molar concentration of ergosterol”
e1“the concentrations of some phenolic compounds and phytoalexins increased in time”
Claim · model-written summary
Ergosterol did not provoke leaf necrosis or cell death.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- ergosterole1
- Outcome
- leaf necrosis or cell deathe1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco cells and plants with nano-molar concentration of ergosterol”
e1“ergosterol did not provoke any leaf necrosis or cell death”
Claim · model-written summary
PR protein accumulation was slower with ergosterol than with cryptogein.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- ergosterole0
- Interactor
- Phytophthora cryptogea
Phytophthora cryptogeae1pathogen - Outcome
- accumulation of PR proteinse1
- Direction
- slowere1
- Comparator
- cryptogeine1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco cells and plants with nano-molar concentration of ergosterol”
e1“accumulation of PR proteins was slower when compared with another well defined elicitor cryptogein from Phytophthora cryptogea”
The same questions in other papers
ergosterol · molecular: expression · Nicotiana tabacum (tobacco)Results agree
1 paper in the same direction as this paper
- positive
ergosterol · molecular: metabolites · Nicotiana tabacum (tobacco)Results agree
1 paper in the same direction as this paper
- positive
nanoparticle · molecular: metabolites · Nicotiana tabacum (tobacco)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
1 paper more in this group
See all 5 papers →nanoparticle · quality: phytochemicals · Nicotiana tabacum (tobacco)Results agree
5 papers in the same direction as this paper
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →nanoparticle · molecular: expression · Nicotiana tabacum (tobacco)Results agree
6 papers in the same direction as this paper
- positive
- positive
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.