Abstract
Abstract Long-distance transport or systemic silencing effects of exogenous biologically active RNA molecules in higher plants have not been reported. Here, we report that cationized bovine serum albumin (cBSA) avidly binds double-stranded beta-glucuronidase RNA (dsGUS RNA) to form nucleic acid–protein nanocomplexes. In our experiments with tobacco and poplar plants, we have successfully demonstrated systemic gene silencing effects of cBSA/dsGUS RNA nanocomplexes when we locally applied the nanocomplexes from the basal ends of leaf petioles or shoots. We have further demonstrated that the cBSA/dsGUS RNA nanocomplexes are highly effective in silencing both the conditionally inducible DR5-GUS gene and the constitutively active 35S-GUS gene in leaf, shoot, and shoot meristem tissues. This cBSA/dsRNA delivery technology may provide a convenient, fast, and inexpensive tool for characterizing gene functions in plants and potentially for in planta gene editing.
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Claims 4
Claim · model-written summary
cBSA avidly binds dsGUS RNA to form nucleic acid–protein nanocomplexes.
- Taxon
- tobacco and poplar
Populus
Nicotiana tabacume1 - Outcome
- avidly binds double-stranded beta-glucuronidase RNA (dsGUS RNA)e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In our experiments with tobacco and poplar plants, we have successfully demonstrated systemic gene silencing effects of cBSA/dsGUS RNA nanocomplexes when we locally applied the nanocomplexes from the basal ends of leaf petioles or shoots”
e3“cationized bovine serum albumin (cBSA) avidly binds double-stranded beta-glucuronidase RNA (dsGUS RNA) to form nucleic acid–protein nanocomplexes”
Claim · model-written summary
Local application of cBSA/dsGUS RNA nanocomplexes at the basal ends of leaf petioles or shoots gave systemic gene silencing in tobacco and poplar.
- Taxon
- tobacco and poplarPopulusNicotiana tabacume1
- Organ / stage
- leaf petioles or shootse1
- Treatment
- locally applied the nanocomplexes from the basal ends of leaf petioles or shootse1
- Gene
- dsGUS RNAe1
- Outcome
- systemic gene silencing effectse1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In our experiments with tobacco and poplar plants, we have successfully demonstrated systemic gene silencing effects of cBSA/dsGUS RNA nanocomplexes when we locally applied the nanocomplexes from the basal ends of leaf petioles or shoots”
Claim · model-written summary
cBSA/dsGUS RNA nanocomplexes were highly effective in silencing the DR5-GUS gene in leaf, shoot and shoot meristem tissues.
- Taxon
- tobacco and poplarPopulusNicotiana tabacume1
- Organ / stage
- leaf, shoot, and shoot meristem tissuese2
- Treatment
- cBSA/dsGUS RNA nanocomplexese2
- Gene
- DR5-GUSe2
- Outcome
- highly effective in silencinge2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In our experiments with tobacco and poplar plants”
e2“We have further demonstrated that the cBSA/dsGUS RNA nanocomplexes are highly effective in silencing both the conditionally inducible DR5-GUS gene and the constitutively active 35S-GUS gene in leaf, shoot, and shoot meristem tissues”
Claim · model-written summary
cBSA/dsGUS RNA nanocomplexes were highly effective in silencing the 35S-GUS gene in leaf, shoot and shoot meristem tissues.
- Taxon
- tobacco and poplarPopulusNicotiana tabacume1
- Organ / stage
- leaf, shoot, and shoot meristem tissuese2
- Treatment
- cBSA/dsGUS RNA nanocomplexese2
- Gene
- 35S-GUSe2
- Outcome
- highly effective in silencinge2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In our experiments with tobacco and poplar plants”
e2“We have further demonstrated that the cBSA/dsGUS RNA nanocomplexes are highly effective in silencing both the conditionally inducible DR5-GUS gene and the constitutively active 35S-GUS gene in leaf, shoot, and shoot meristem tissues”
The same questions in other papers
nanoparticle · molecular: expression · Nicotiana tabacum (tobacco)Results agree
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- positive
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See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.