Abstract
Synthesis and accumulation of plant oils in the entire vegetative biomass offers the potential to deliver yields surpassing those of oilseed crops. However, current levels still fall well short of those typically found in oilseeds. Here we show how transcriptome and biochemical analyses pointed to a futile cycle in a previously established Nicotiana tabacum line, accumulating up to 15% (dry weight) of the storage lipid triacylglycerol in leaf tissue. To overcome this metabolic bottleneck, we either silenced the SDP1 lipase or overexpressed the Arabidopsis thaliana LEC2 transcription factor in this transgenic background. Both strategies independently resulted in the accumulation of 30-33% triacylglycerol in leaf tissues. Our results demonstrate that the combined optimization of de novo fatty acid biosynthesis, storage lipid assembly and lipid turnover in leaf tissue results in a major overhaul of the plant central carbon allocation and lipid metabolism. The resulting further step changes in oil accumulation in the entire plant biomass offers the possibility of delivering yields that outperform current oilseed crops.
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Claims 4
Claim · model-written summary
Transcriptome and biochemical analyses pointed to a futile cycle in a previously established tobacco line.
- Taxon
- Nicotiana tabacum
Nicotiana tabacume0 - Material
- previously established Nicotiana tabacum linee0
- Outcome
- futile cyclee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcriptome and biochemical analyses pointed to a futile cycle in a previously established Nicotiana tabacum line”
Claim · model-written summary
Silencing the SDP1 lipase in tobacco leaves increased triacylglycerol accumulation to 30-33% of leaf dry weight.
- Taxon
- Nicotiana tabacumNicotiana tabacume0
- Organ / stage
- leaf tissuese3
- Treatment
- silenced the SDP1 lipasee1
- Gene
- SDP1 lipasee1
- Outcome
- triacylglycerol in leaf tissuese3
- Direction
- accumulatione3
- Value
- 30-33%e3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcriptome and biochemical analyses pointed to a futile cycle in a previously established Nicotiana tabacum line”
e1“we either silenced the SDP1 lipase”
e3“Both strategies independently resulted in the accumulation of 30-33% triacylglycerol in leaf tissues”
Claim · model-written summary
Overexpressing Arabidopsis LEC2 in tobacco leaves increased triacylglycerol accumulation to 30-33%.
- Taxon
- Nicotiana tabacumNicotiana tabacume0
- Organ / stage
- leaf tissuese3
- Treatment
- overexpressed the Arabidopsis thaliana LEC2 transcription factore2
- Gene
- Arabidopsis thaliana LEC2e2
- Outcome
- triacylglycerol in leaf tissuese3
- Direction
- accumulatione3
- Value
- 30-33%e3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcriptome and biochemical analyses pointed to a futile cycle in a previously established Nicotiana tabacum line”
e2“overexpressed the Arabidopsis thaliana LEC2 transcription factor”
e3“Both strategies independently resulted in the accumulation of 30-33% triacylglycerol in leaf tissues”
Claim · model-written summary
Combined optimization of fatty acid biosynthesis, storage lipid assembly and lipid turnover in leaf tissue overhauls central carbon allocation and lipid metabolism.
- Taxon
- Nicotiana tabacumNicotiana tabacume0
- Organ / stage
- leaf tissuee4
- Treatment
- combined optimization of de novo fatty acid biosynthesis, storage lipid assembly and lipid turnovere4
- Outcome
- major overhaul of the plant central carbon allocation and lipid metabolisme4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcriptome and biochemical analyses pointed to a futile cycle in a previously established Nicotiana tabacum line”
e4“the combined optimization of de novo fatty acid biosynthesis, storage lipid assembly and lipid turnover in leaf tissue results in a major overhaul of the plant central carbon allocation and lipid metabolism”
The same questions in other papers
loss of function · molecular: metabolites · Nicotiana tabacum (tobacco)Positive and negative results
9 papers in the same direction as this paper · 14 in the opposite direction
- negative
- no effect
- negative
20 papers more in this group
See all 24 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.