Abstract
High levels of salinity induce serious oxidative damage in plants. Flavonoids, as antioxidants, have important roles in ROS scavenging. In the present study, the tobacco R2R3 MYB type repressor, NtMYB4, was isolated and characterized. The expression of NtMYB4 was suppressed by salinity. Overexpression of NtMYB4 reduced the salt tolerance in transgenic tobacco plants. NtMYB4 repressed the promoter activity of NtCHS1 and negatively regulated its expression. Rutin accumulation was significantly decreased in NtMYB4 overexpressing transgenic plants and NtCHS1 RNAi silenced transgenic plants. Moreover, high H2O2 and O2- contents were detected in both types of rutin-reduced transgenic plants under high salt stress. In addition, exogenous rutin supplementation effectively scavenged ROS (H2O2 and O2-) and improved the salt tolerance of the rutin-reduced transgenic plants. In contrast, NtCHS1 overexpressing plants had increased rutin accumulation, lower H2O2 and O2- contents, and higher tolerance to salinity. These results suggested that tobacco NtMYB4 acts as a salinity response repressor and negatively regulates NtCHS1 expression, which results in the reduced flavonoid accumulation and weakened ROS-scavenging ability under salt stress.
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Claims 11
Claim · model-written summary
Salinity suppressed NtMYB4 expression.
- Taxon
- tobacco
Nicotiana tabacume1 - Treatment
- salinitye0
- Gene
- NtMYB4e0
- Outcome
- expression of NtMYB4e0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of NtMYB4 was suppressed by salinity.”
e1“the tobacco R2R3 MYB type repressor, NtMYB4”
Claim · model-written summary
NtMYB4 overexpression reduced salt tolerance in transgenic tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- Overexpression of NtMYB4e0
- Gene
- NtMYB4e0
- Outcome
- salt tolerancee0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of NtMYB4 reduced the salt tolerance in transgenic tobacco plants.”
Claim · model-written summary
NtMYB4 repressed NtCHS1 promoter activity and expression.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- NtMYB4e0
- Gene
- NtCHS1e0
- Outcome
- promoter activity of NtCHS1 and negatively regulated its expressione0
- Direction
- repressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NtMYB4 repressed the promoter activity of NtCHS1 and negatively regulated its expression.”
e1“the tobacco R2R3 MYB type repressor, NtMYB4”
Claim · model-written summary
NtMYB4 overexpression significantly decreased rutin accumulation.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- NtMYB4 overexpressinge0
- Gene
- NtMYB4e0
- Outcome
- Rutin accumulatione0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rutin accumulation was significantly decreased in NtMYB4 overexpressing transgenic plants”
e1“the tobacco R2R3 MYB type repressor, NtMYB4”
Claim · model-written summary
NtCHS1 silencing significantly decreased rutin accumulation.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- NtCHS1 RNAi silencede0
- Gene
- NtCHS1e0
- Outcome
- Rutin accumulatione0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rutin accumulation was significantly decreased in NtMYB4 overexpressing transgenic plants and NtCHS1 RNAi silenced transgenic plants.”
e1“the tobacco R2R3 MYB type repressor, NtMYB4”
Claim · model-written summary
Both rutin-reduced transgenic types accumulated high H2O2 and O2- under high salt stress.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- high salt stresse0
- Outcome
- H2O2 and O2- contentse0
- Direction
- highe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“high H2O2 and O2- contents were detected in both types of rutin-reduced transgenic plants under high salt stress”
e1“the tobacco R2R3 MYB type repressor, NtMYB4”
Claim · model-written summary
Exogenous rutin scavenged ROS in rutin-reduced plants.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- exogenous rutin supplementatione0
- Outcome
- ROS (H2O2 and O2-)e0
- Direction
- scavengede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous rutin supplementation effectively scavenged ROS (H2O2 and O2-)”
e1“the tobacco R2R3 MYB type repressor, NtMYB4”
Claim · model-written summary
Exogenous rutin improved salt tolerance in rutin-reduced plants.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- exogenous rutin supplementatione0
- Outcome
- salt tolerancee0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous rutin supplementation effectively scavenged ROS (H2O2 and O2-) and improved the salt tolerance of the rutin-reduced transgenic plants”
e1“the tobacco R2R3 MYB type repressor, NtMYB4”
Claim · model-written summary
NtCHS1 overexpression increased rutin accumulation.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- NtCHS1 overexpressinge0
- Gene
- NtCHS1e0
- Outcome
- rutin accumulatione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NtCHS1 overexpressing plants had increased rutin accumulation”
e1“the tobacco R2R3 MYB type repressor, NtMYB4”
Claim · model-written summary
NtCHS1 overexpression lowered H2O2 and O2- content.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- NtCHS1 overexpressinge0
- Gene
- NtCHS1e0
- Outcome
- H2O2 and O2- contentse0
- Direction
- lowere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NtCHS1 overexpressing plants had increased rutin accumulation, lower H2O2 and O2- contents”
e1“the tobacco R2R3 MYB type repressor, NtMYB4”
Claim · model-written summary
NtCHS1 overexpression increased salinity tolerance.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- NtCHS1 overexpressinge0
- Gene
- NtCHS1e0
- Outcome
- tolerance to salinitye0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NtCHS1 overexpressing plants had increased rutin accumulation, lower H2O2 and O2- contents, and higher tolerance to salinity”
e1“the tobacco R2R3 MYB type repressor, NtMYB4”
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