Abstract
On the basis of the biological compounds that they metabolize, plant peroxidases have long been implicated in plant growth, cell wall biogenesis, lignification, and host defenses. Transgenic tobacco (Nicotiana tabacum L.) plants that underexpress anionic peroxidase were generated using antisense RNA. The antisense RNA was found to be specific for the anionic isoenzyme and highly effective, reducing endogenous transcript levels and total peroxidase activity by as much as 1600-fold. Antisense-transformed plants appeared normal at initial observation; however, growth studies showed that plants with reduced peroxidase activity grow taller and flower sooner than control plants. In contrast, previously transformed plants overproducing anionic peroxidase were shorter and flowered later than controls. Axillary buds were more developed in antisense-transformed plants and less developed in plants overproducing this enzyme. It was found that the lignin content in leaf, stem, and root was unchanged in antisense-transformed plants, which does not support a role for anionic peroxidase in the lignification of secondary xylem vessels. However, studies of wounded tissue show some reduction in wound-induced deposition of lignin-like polymers. The data support a possible role for tobacco anionic peroxidase in host defenses but not without a reduction in growth potential.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
Antisense RNA specifically suppressed anionic peroxidase transcripts and total activity by up to 1600-fold.
- Taxon
- tobacco
Nicotiana tabacume1 - Treatment
- antisense RNAe0
- Gene
- anionic isoenzymee0
- Outcome
- endogenous transcript levels and total peroxidase activitye0
- Direction
- reducinge0
- Value
- 1600-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The antisense RNA was found to be specific for the anionic isoenzyme and highly effective, reducing endogenous transcript levels and total peroxidase activity by as much as 1600-fold”
e1“Transgenic tobacco (Nicotiana tabacum L.) plants”
Claim · model-written summary
Antisense-transformed plants initially appeared normal.
- Taxon
- tobaccoNicotiana tabacume1
- Material
- Antisense-transformed plantse0
- Outcome
- appeared normale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antisense-transformed plants appeared normal at initial observation”
e1“Transgenic tobacco (Nicotiana tabacum L.) plants”
Claim · model-written summary
Plants with reduced peroxidase activity grew taller and flowered sooner than controls.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- reduced peroxidase activitye0
- Outcome
- grow taller and flower soonere0
- Direction
- taller and flower soonere0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plants with reduced peroxidase activity grow taller and flower sooner than control plants”
e1“Transgenic tobacco (Nicotiana tabacum L.) plants”
Claim · model-written summary
Plants overproducing anionic peroxidase were shorter and flowered later than controls.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- overproducing anionic peroxidasee0
- Outcome
- shorter and flowered latere0
- Direction
- shorter and flowered latere0
- Comparator
- controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plants overproducing anionic peroxidase were shorter and flowered later than controls”
e1“Transgenic tobacco (Nicotiana tabacum L.) plants”
Claim · model-written summary
Axillary buds were more developed in antisense-transformed plants.
- Taxon
- tobaccoNicotiana tabacume1
- Material
- antisense-transformed plantse0
- Outcome
- Axillary budse0
- Direction
- more developede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Axillary buds were more developed in antisense-transformed plants”
e1“Transgenic tobacco (Nicotiana tabacum L.) plants”
Claim · model-written summary
Axillary buds were less developed in anionic peroxidase overproducers.
- Taxon
- tobaccoNicotiana tabacume1
- Material
- plants overproducing this enzymee0
- Outcome
- Axillary budse0
- Direction
- less developede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Axillary buds were more developed in antisense-transformed plants and less developed in plants overproducing this enzyme”
e1“Transgenic tobacco (Nicotiana tabacum L.) plants”
Claim · model-written summary
Lignin content in leaves, stems and roots was unchanged in antisense plants.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- antisense-transformed plantse0
- Outcome
- lignin content in leaf, stem, and roote0
- Direction
- unchangede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the lignin content in leaf, stem, and root was unchanged in antisense-transformed plants”
e1“Transgenic tobacco (Nicotiana tabacum L.) plants”
Claim · model-written summary
Wounded tissue showed some reduction in wound-induced lignin-like polymer deposition.
- Taxon
- tobaccoNicotiana tabacume1
- Organ / stage
- wounded tissuee0
- Outcome
- wound-induced deposition of lignin-like polymerse0
- Direction
- some reductione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“studies of wounded tissue show some reduction in wound-induced deposition of lignin-like polymers”
e1“Transgenic tobacco (Nicotiana tabacum L.) plants”
The same questions in other papers
loss of function · molecular: metabolites · Nicotiana tabacum (tobacco)Positive and negative results
13 papers in the same direction as this paper · 10 in the opposite direction
- no effect
- positive
- positive
20 papers more in this group
See all 24 papers →loss of function · molecular: expression · Nicotiana tabacum (tobacco)Positive and negative results
13 papers in the same direction as this paper · 36 in the opposite direction
- negative
- negative
- negative
46 papers more in this group
See all 50 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.