Abstract
The change in the developmental pathway of microspores from gametophytic to sporophytic is induced by stress during pretreatment of spikes and anthers. In our experiments, anther culture of three barley cultivars was tested with regard to the effect of chilling at 4 degrees C for 28 days, starvation in 0.3 M mannitol solution for 4 days, and a combination of both methods. Chilling was shown to increase embryo/callus formation, while mannitol treatment favoured plant development, including development of green plants; simultaneous application of the two stress factors for 4 days proved to be ineffective. The tested cultivars exhibited a similar ability (calculated per 100 transferred embryos/calli) to develop plants without pretreatment; however, their responses to stress varied greatly. The collected data indicate that mannitol pretreatment, as compared to chilling, is more efficient in responsive cultivars.
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Claims 6
Claim · model-written summary
Chilling increased embryo or callus formation.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaret0 - Treatment
- Chillinge0
- Outcome
- embryo/callus formatione0
- Direction
- increaseinferrede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Chilling was shown to increase embryo/callus formation”
t0“barley”
Claim · model-written summary
Mannitol favoured plant development, including green plants.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- mannitol treatmente0
- Outcome
- plant development, including development of green plantse0
- Direction
- favourede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mannitol treatment favoured plant development, including development of green plants”
t0“barley”
Claim · model-written summary
Simultaneous chilling and mannitol for four days was ineffective.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- simultaneous application of the two stress factors for 4 dayse0
- Outcome
- ineffectivee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“simultaneous application of the two stress factors for 4 days proved to be ineffective”
t0“barley”
Claim · model-written summary
Without pretreatment, tested cultivars had similar plant development per 100 embryos or calli.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- similar ability (calculated per 100 transferred embryos/calli) to develop plantse0
- Qualifier
- without pretreatmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The tested cultivars exhibited a similar ability (calculated per 100 transferred embryos/calli) to develop plants without pretreatment”
t0“barley”
Claim · model-written summary
Cultivar responses to stress varied greatly.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- responses to stresse0
- Direction
- varied greatlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“their responses to stress varied greatly”
t0“barley”
Claim · model-written summary
Mannitol pretreatment was more efficient than chilling in responsive cultivars.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- mannitol pretreatmente0
- Outcome
- efficiente0
- Direction
- more efficiente0
- Qualifier
- responsive cultivarse0
- Comparator
- chillinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mannitol pretreatment, as compared to chilling, is more efficient in responsive cultivars”
t0“barley”
The same questions in other papers
mannitol · growth · Hordeum vulgare (barley)Positive and negative results
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7 papers in the same direction as this paper · 11 in the opposite direction
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See all 19 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.