Abstract
Aim. Ambryoidogenesis, or somatic embryogenesis, is known to be the most efficient mode of plant regeneration in plant cell, tissue and organ culture. The investigation was aimed to elucidate effects of trophic and osmogenic components of inductive medium on the frequency of direct embryoidogenesis in spring barley anther culture in vitro and to determine mechanism of morphogenesis improvement coursed by chemically modified starch D-5aM used as a gelling agent instead of agar. Methods. Anthers of DH-line with a high androgenetic capacity were inoculated on inductive media containing N6 macro-, MS micronutrients, organic supplements, maltose or mannitol (0.3 M) and solidified with agar or chemically modified starch. Results. A positive effect of combination of high maltose content and chemically modified starch on the induction and regeneration processes in spring barley anther culture in vitro was confirmed. It was also shown that mannitol didn’t keep any growth or development processes going in barley anther and embryo culture, but at the same time this substance had no toxic effect. Conclusions. In order to achieve a high frequency of induction in spring barley anther culture, it is necessary to use medium containing maltose – a low weight component both with trophic and with osmotic activity. Keywords: Hordeum vulgare L., anther culture in vitro, mannitol, maltose, embryo formation, plant regeneration.
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Claims 3
Claim · model-written summary
High maltose with chemically modified starch improved induction and regeneration in spring barley anther culture.
- Taxon
- spring barley
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Hordeum vulgaree0 - Organ / stage
- anthere0
- Treatment
- combination of high maltose content and chemically modified starche0
- Outcome
- induction and regeneration processese0
- Direction
- positive effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“A positive effect of combination of high maltose content and chemically modified starch on the induction and regeneration processes in spring barley anther culture in vitro was confirmed”
Claim · model-written summary
Mannitol did not sustain growth or development in barley anther and embryo culture.
- Taxon
- barleyHordeum vulgaree0
- Organ / stage
- anther and embryoe0
- Treatment
- mannitole0
- Outcome
- growth or development processese0
- Direction
- didn’t keepe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mannitol didn’t keep any growth or development processes going in barley anther and embryo culture”
Claim · model-written summary
Mannitol had no toxic effect in barley culture.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- mannitole1
- Outcome
- toxic effecte0
- Direction
- no toxic effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but at the same time this substance had no toxic effect”
t0“spring barley anther culture in vitro”
e1“mannitol didn’t keep any growth or development processes going in barley anther and embryo culture”
The same questions in other papers
mannitol · growth · Hordeum vulgare (barley)Positive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →maltose · growth · Hordeum vulgare (barley)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.