Abstract
Two cDNA clones, designated ERD10 and EDR14, isolated from a cDNA library from one-hour-dehydrated plants of Arabidopsis thaliana L. were sequenced and characterized. The predicted ER10 and ERD14 polypeptides have a compositional bias towards Glu (19.62% and 21.08%, respectively) and Lys (16.15% and 18.38%, respectively) and both lack Trp and Cys residues. The amino acid sequences deduced from of both cDNAs are very similar to those of Group II LEA (late embryogenesis abundant) proteins, a class of proteins that accumulate late in embryogenesis and whose gene expression is induced by the plant hormone abscisic acid (ABA). Genomic Southern blot analysis suggested the existence of an ERD10-related gene. The expression of the genes that correspond to ERD10 and ERD14 was strongly induced in rosette plants of Arabidopsis within 1 h of dehydration and elevated levels of transcripts were detected during 24 h of dehydration. Northern blot analysis of the cold-induced expression of both the erd10 and erd14 genes revealed a two-step induction process. Early induction occurred within 1 h and secondary induction occurred 5 h after the beginning of cold stress. Application of ABA induced the expression of both genes, but application of 2,4-D, BA, ABA and GA3 did not affect the expression of these genes. In bolting plants, both ERD10 and ERD14 transcripts were detected in stems, cauline leaves, roots and flowers, but their levels decreased during seed formation and only low levels of the transcripts were detected in seeds at the late-mature stage.
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Claims 14
Claim · model-written summary
Dehydration strongly induced ERD10 and ERD14 expression in rosette plants within 1 h.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Organ / stage
- rosette plantse1
- Treatment
- dehydratione1
- Gene
- ERD10 and ERD14e1
- Outcome
- expression of the genes that correspond to ERD10 and ERD14e1
- Direction
- strongly inducede1
- Qualifier
- within 1 he1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isolated from a cDNA library from one-hour-dehydrated plants of Arabidopsis thaliana L”
e1“The expression of the genes that correspond to ERD10 and ERD14 was strongly induced in rosette plants of Arabidopsis within 1 h of dehydration”
Claim · model-written summary
Dehydration kept ERD10 and ERD14 transcript levels elevated for 24 h.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- dehydratione2
- Outcome
- transcriptse2
- Direction
- elevatede2
- Qualifier
- during 24 he2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isolated from a cDNA library from one-hour-dehydrated plants of Arabidopsis thaliana L”
e2“elevated levels of transcripts were detected during 24 h of dehydration”
Claim · model-written summary
Cold stress induced a two-step expression of erd10 and erd14, with secondary induction 5 h after the start of stress.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- cold stresse4
- Gene
- erd10 and erd14e3
- Outcome
- expression of both the erd10 and erd14 genese3
- Direction
- two-step inductione3
- Qualifier
- secondary induction occurred 5 he4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isolated from a cDNA library from one-hour-dehydrated plants of Arabidopsis thaliana L”
e3“Northern blot analysis of the cold-induced expression of both the erd10 and erd14 genes revealed a two-step induction process”
e4“Early induction occurred within 1 h and secondary induction occurred 5 h after the beginning of cold stress”
Claim · model-written summary
Application of ABA induced ERD10 and ERD14 expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Application of ABAe5
- Outcome
- expression of both genese5
- Direction
- inducede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isolated from a cDNA library from one-hour-dehydrated plants of Arabidopsis thaliana L”
e5“Application of ABA induced the expression of both genes”
Claim · model-written summary
Application of 2,4-D and BA did not affect ERD10 and ERD14 expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- 2,4-D, BAe6
- Outcome
- expression of these genese6
- Direction
- did not affecte6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isolated from a cDNA library from one-hour-dehydrated plants of Arabidopsis thaliana L”
e6“application of 2,4-D, BA, ABA and GA3 did not affect the expression of these genes”
Claim · model-written summary
Application of GA3 did not affect ERD10 and ERD14 expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- GA3e6
- Outcome
- expression of these genese6
- Direction
- did not affecte6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isolated from a cDNA library from one-hour-dehydrated plants of Arabidopsis thaliana L”
e6“application of 2,4-D, BA, ABA and GA3 did not affect the expression of these genes”
Claim · model-written summary
ERD10 and ERD14 transcripts were detected in stems, cauline leaves, roots and flowers of bolting plants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Organ / stage
- stems, cauline leaves, roots and flowerse7
- Gene
- ERD10 and ERD14e7
- Outcome
- ERD10 and ERD14 transcriptse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isolated from a cDNA library from one-hour-dehydrated plants of Arabidopsis thaliana L”
e7“In bolting plants, both ERD10 and ERD14 transcripts were detected in stems, cauline leaves, roots and flowers”
Claim · model-written summary
ERD10 and ERD14 transcript levels decreased during seed formation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Organ / stage
- seed formatione2
- Gene
- ERD10 and ERD14e7
- Outcome
- levelse2
- Direction
- decreasede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isolated from a cDNA library from one-hour-dehydrated plants of Arabidopsis thaliana L”
e7“In bolting plants, both ERD10 and ERD14 transcripts were detected in stems, cauline leaves, roots and flowers”
e2“their levels decreased during seed formation”
Claim · model-written summary
Only low ERD10 and ERD14 transcript levels were detected in late-mature seeds.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Organ / stage
- seeds at the late-mature stagee1
- Outcome
- levels of the transcriptse1
- Qualifier
- lowe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isolated from a cDNA library from one-hour-dehydrated plants of Arabidopsis thaliana L”
e1“only low levels of the transcripts were detected in seeds at the late-mature stage”
Claim · model-written summary
A genomic Southern blot suggested an ERD10-related gene exists in Arabidopsis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- ERD10-related genee1
- Outcome
- existence of an ERD10-related genee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isolated from a cDNA library from one-hour-dehydrated plants of Arabidopsis thaliana L”
e1“Genomic Southern blot analysis suggested the existence of an ERD10-related gene”
Claim · model-written summary
The predicted ERD10 and ERD14 polypeptides show a compositional bias towards Glu, at 19.62% and 21.08%.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Outcome
- compositional bias towards Glue1
- Value
- 19.62% and 21.08%e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isolated from a cDNA library from one-hour-dehydrated plants of Arabidopsis thaliana L”
e1“compositional bias towards Glu (19.62% and 21.08%, respectively) and Lys (16.15% and 18.38%, respectively)”
Claim · model-written summary
The predicted ERD10 and ERD14 polypeptides show a compositional bias towards Lys, at 16.15% and 18.38%.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Outcome
- Lyse1
- Value
- 16.15% and 18.38%e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isolated from a cDNA library from one-hour-dehydrated plants of Arabidopsis thaliana L”
e1“compositional bias towards Glu (19.62% and 21.08%, respectively) and Lys (16.15% and 18.38%, respectively)”
Claim · model-written summary
The predicted ERD10 and ERD14 polypeptides lack Trp and Cys residues.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Outcome
- lack Trp and Cys residuese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isolated from a cDNA library from one-hour-dehydrated plants of Arabidopsis thaliana L”
e1“both lack Trp and Cys residues”
Claim · model-written summary
The deduced amino acid sequences of both cDNAs are very similar to Group II LEA proteins.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Outcome
- amino acid sequencese1
- Qualifier
- very similar to those of Group II LEAe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isolated from a cDNA library from one-hour-dehydrated plants of Arabidopsis thaliana L”
e1“The amino acid sequences deduced from of both cDNAs are very similar to those of Group II LEA (late embryogenesis abundant) proteins”
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2,4-D · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- positive
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.