Abstract
Phytohormones are indispensable factors regulating plant cell dedifferentiation. In this paper, different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin (KIN) were incorporated in the culture medium and the anatomy of dedifferentiated cells prior to callus formation from Arabidopsis thaliana petiole explants was examined. The results indicated that the cytoplasm of parenchyma cells in the vascular bundle gradually became denser with time of culture only if 2,4-D was included in the medium. The WUSCHEL (WUS) gene was expressed in derivative cells of the vascular bundle after culture for 24 h in the presence of 2,4-D and there was no obvious signal in these cells of cultured petioles with KIN alone. These results suggest that 2,4-D plays an important role in the process of dedifferentiation of vascular bundle cells in Arabidopsis petioles and KIN has no obvious effect on it.
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Claims 5
Claim · model-written summary
With 2,4-D, vascular bundle parenchyma cytoplasm became denser over culture time.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae1 - Organ / stage
- petiolee1
- Treatment
- 2,4-De0
- Outcome
- cytoplasm of parenchyma cells in the vascular bundlee0
- Direction
- became densere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the cytoplasm of parenchyma cells in the vascular bundle gradually became denser with time of culture only if 2,4-D was included in the medium”
e1“Arabidopsis thaliana petiole explants”
Claim · model-written summary
WUSCHEL expression appeared in vascular bundle derivative cells after 24 h with 2,4-D.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- 2,4-De0
- Gene
- WUSCHEL (WUS)e0
- Outcome
- gene was expressed in derivative cells of the vascular bundlee0
- Qualifier
- 24 he0
- Comparator
- KIN alonee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The WUSCHEL (WUS) gene was expressed in derivative cells of the vascular bundle after culture for 24 h in the presence of 2,4-D and there was no obvious signal in these cells of cultured petioles with KIN alone.”
e1“Arabidopsis thaliana petiole explants”
Claim · model-written summary
KIN alone produced no obvious WUSCHEL signal in cultured petiole cells.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae2
- Organ / stage
- cultured petiolese0
- Treatment
- KIN alonee0
- Gene
- WUSCHEL (WUS)e1
- Outcome
- no obvious signale0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there was no obvious signal in these cells of cultured petioles with KIN alone”
e1“The WUSCHEL (WUS) gene was expressed”
e2“Arabidopsis thaliana petiole explants”
Claim · model-written summary
The authors infer that 2,4-D plays an important role in vascular bundle cell dedifferentiation.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- petiolese0
- Treatment
- 2,4-De0
- Outcome
- dedifferentiation of vascular bundle cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“2,4-D plays an important role in the process of dedifferentiation of vascular bundle cells in Arabidopsis petioles”
Claim · model-written summary
The authors infer that KIN has no obvious effect on vascular bundle cell dedifferentiation.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- KINe0
- Outcome
- dedifferentiation of vascular bundle cellse1
- Direction
- no obvious effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“KIN has no obvious effect on it”
e1“the process of dedifferentiation of vascular bundle cells in Arabidopsis petioles”
The same questions in other papers
2,4-D · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.