Abstract
The effects of colchcine on the survival percentage and on the regeneration frequency were tested in culture microspore from 4 cultivars of barley. The results showed that: CPW solution containing mannitol of 0.3 mol/L and colchicine 10 mg/L used as isolation and pretreatment solution was essential for microspore embryogenesis(Table 3); the survival percentage of isolated microspores could be increased by isolation solution supplemented with colchicine 10 mg/L(Table 1); pretreatment of freshly isolated microspores with CPW solution containing mannitol 0.3 mol/L and colchicine 10 mg/L (25℃, 2 d) could decrease the death rate of microspores after culture for 3 d(Table 2)and increased green plant regeneration(Table 4). According to the comparison, the microspore embryogenesis and green plant regeneration frequency were increased by addition of 10 mg/L of colchicine to isolation and pretreatment solution.
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Claims 5
Claim · model-written summary
The mannitol and colchicine solution was essential for microspore embryogenesis.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaret0 - Treatment
- CPW solution containing mannitol of 0.3 mol/L and colchicine 10 mg/Le0
- Outcome
- microspore embryogenesise0
- Direction
- essentiale0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“CPW solution containing mannitol of 0.3 mol/L and colchicine 10 mg/L used as isolation and pretreatment solution was essential for microspore embryogenesis”
t0“Barley”
Claim · model-written summary
Colchicine in isolation solution increased isolated microspore survival.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- isolation solution supplemented with colchicine 10 mg/Le0
- Outcome
- survival percentage of isolated microsporese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the survival percentage of isolated microspores could be increased by isolation solution supplemented with colchicine 10 mg/L”
t0“Barley”
Claim · model-written summary
Mannitol and colchicine pretreatment decreased microspore death after three days.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- pretreatment of freshly isolated microspores with CPW solution containing mannitol 0.3 mol/L and colchicine 10 mg/Le0
- Outcome
- death rate of microsporese0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pretreatment of freshly isolated microspores with CPW solution containing mannitol 0.3 mol/L and colchicine 10 mg/L (25℃, 2 d) could decrease the death rate of microspores after culture for 3 d”
t0“Barley”
Claim · model-written summary
Pretreatment increased green plant regeneration.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- pretreatment of freshly isolated microspores with CPW solution containing mannitol 0.3 mol/L and colchicine 10 mg/Le0
- Outcome
- green plant regeneratione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pretreatment of freshly isolated microspores with CPW solution containing mannitol 0.3 mol/L and colchicine 10 mg/L (25℃, 2 d) could decrease the death rate of microspores after culture for 3 d(Table 2)and increased green plant regeneration”
t0“Barley”
Claim · model-written summary
Adding colchicine increased microspore embryogenesis and green plant regeneration frequency.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- addition of 10 mg/L of colchicine to isolation and pretreatment solutione0
- Outcome
- microspore embryogenesis and green plant regeneration frequencye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the microspore embryogenesis and green plant regeneration frequency were increased by addition of 10 mg/L of colchicine to isolation and pretreatment solution”
t0“Barley”
The same questions in other papers
colchicine · growth · Hordeum vulgare (barley)Positive and negative results
1 in the opposite direction
- negative
mannitol · growth · Hordeum vulgare (barley)Positive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- negative
- negative
- negative
2 papers more in this group
See all 6 papers →mannitol · survival · Hordeum vulgare (barley)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.