Abstract
An experiment was conducted to induce tetraploidy in three diploid barley varieties (Martin, Rihane and Manel) through different colchicines treatments. Colchicine was added for three different concentrations at three different stages of plant development i.e. on seed (0.05% for 48 hours), on pre-germinated seeds (0.1% for 2 hours) and on three leaf stage (0.1% for 16 hours). Colchicine application reduced significantly germination percentage and viability of plants. Seed germination was completely inhibited in Martin, while a reduction of 20% and 30% for germination percentage compared to control was recorded in varieties Manel and Rihane, respectively at 0.1% colchicine concentration. Ploidy evaluation showed no tetraploidy in all the three tested varieties by colchicine application of 0.05% for 48 hours on seeds and 0.1% for 2 hours on pre-germinated seeds. However, tetraploid plants were produced only by treatment with 0.1% for 16 hours of seedlings. The percentages of plants were 40%, 44% and 100% for Rihane, Manel and Martin, respectively. Cytological analyses showed the increase of chromosome numbers from 2n=2x=14 to 2n=4x=28. The increase of ploidy levels caused major changes in some morphological traits. In fact, the induced tetraploids in barley was accompanied by significant (P<0.01) decrease in plant height, tiller height, leaf number and leaf length compared to diploid control plants. colchicine treatment induce successfully the production of tetraploid barley plants and could be used in breeding programs.
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Claims 13
Claim · model-written summary
Colchicine application significantly reduced germination percentage and viability of barley plants.
- Taxon
- barley
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Hordeum vulgaree0 - Treatment
- Colchicine applicatione1
- Outcome
- germination percentage and viability of plantse1
- Direction
- reducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“An experiment was conducted to induce tetraploidy in three diploid barley varieties (Martin, Rihane and Manel) through different colchicines treatments”
e1“Colchicine application reduced significantly germination percentage and viability of plants”
Claim · model-written summary
In Martin, 0.1% colchicine completely inhibited seed germination.
- Taxon
- barleyHordeum vulgaree0
- Material
- Martine2
- Treatment
- 0.1% colchicine concentratione2
- Outcome
- Seed germinatione2
- Direction
- completely inhibitede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An experiment was conducted to induce tetraploidy in three diploid barley varieties (Martin, Rihane and Manel) through different colchicines treatments”
e2“Seed germination was completely inhibited in Martin, while a reduction of 20% and 30% for germination percentage compared to control was recorded in varieties Manel and Rihane, respectively at 0.1% colchicine concentration”
Claim · model-written summary
In Manel, 0.1% colchicine reduced germination percentage by 20% compared to control.
- Taxon
- barleyHordeum vulgaree0
- Material
- Manele2
- Treatment
- 0.1% colchicine concentratione2
- Outcome
- germination percentagee2
- Direction
- reductione2
- Value
- 20%e2
- Comparator
- controle2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An experiment was conducted to induce tetraploidy in three diploid barley varieties (Martin, Rihane and Manel) through different colchicines treatments”
e2“Seed germination was completely inhibited in Martin, while a reduction of 20% and 30% for germination percentage compared to control was recorded in varieties Manel and Rihane, respectively at 0.1% colchicine concentration”
Claim · model-written summary
In Rihane, 0.1% colchicine reduced germination percentage by 30% compared to control.
- Taxon
- barleyHordeum vulgaree0
- Material
- Rihanee2
- Treatment
- 0.1% colchicine concentratione2
- Outcome
- germination percentagee2
- Direction
- reductione2
- Value
- 30%e2
- Comparator
- controle2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An experiment was conducted to induce tetraploidy in three diploid barley varieties (Martin, Rihane and Manel) through different colchicines treatments”
e2“Seed germination was completely inhibited in Martin, while a reduction of 20% and 30% for germination percentage compared to control was recorded in varieties Manel and Rihane, respectively at 0.1% colchicine concentration”
Claim · model-written summary
Colchicine at 0.05% for 48 hours on seeds produced no tetraploid barley plants.
- Taxon
- barleyHordeum vulgaree0
- Treatment
- colchicine application of 0.05% for 48 hours on seedse3
- Outcome
- tetraploidye3
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An experiment was conducted to induce tetraploidy in three diploid barley varieties (Martin, Rihane and Manel) through different colchicines treatments”
e3“Ploidy evaluation showed no tetraploidy in all the three tested varieties by colchicine application of 0.05% for 48 hours on seeds and 0.1% for 2 hours on pre-germinated seeds”
Claim · model-written summary
Colchicine at 0.1% for 2 hours on pre-germinated seeds produced no tetraploid barley plants.
- Taxon
- barleyHordeum vulgaree0
- Treatment
- 0.1% for 2 hours on pre-germinated seedse3
- Outcome
- tetraploidye3
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An experiment was conducted to induce tetraploidy in three diploid barley varieties (Martin, Rihane and Manel) through different colchicines treatments”
e3“Ploidy evaluation showed no tetraploidy in all the three tested varieties by colchicine application of 0.05% for 48 hours on seeds and 0.1% for 2 hours on pre-germinated seeds”
Claim · model-written summary
Colchicine at 0.1% for 16 hours on seedlings produced 40% tetraploid plants in Rihane.
- Taxon
- barleyHordeum vulgaree0
- Material
- Rihanee5
- Treatment
- 0.1% for 16 hours of seedlingse4
- Outcome
- tetraploid plantse4
- Direction
- increaseinferrede4
- Value
- 40%e5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An experiment was conducted to induce tetraploidy in three diploid barley varieties (Martin, Rihane and Manel) through different colchicines treatments”
e4“However, tetraploid plants were produced only by treatment with 0.1% for 16 hours of seedlings”
e5“The percentages of plants were 40%, 44% and 100% for Rihane, Manel and Martin, respectively”
Claim · model-written summary
Colchicine at 0.1% for 16 hours on seedlings produced 44% tetraploid plants in Manel.
- Taxon
- barleyHordeum vulgaree0
- Material
- Manele5
- Treatment
- 0.1% for 16 hours of seedlingse4
- Outcome
- tetraploid plantse4
- Direction
- increaseinferrede4
- Value
- 44%e5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An experiment was conducted to induce tetraploidy in three diploid barley varieties (Martin, Rihane and Manel) through different colchicines treatments”
e4“However, tetraploid plants were produced only by treatment with 0.1% for 16 hours of seedlings”
e5“The percentages of plants were 40%, 44% and 100% for Rihane, Manel and Martin, respectively”
Claim · model-written summary
Colchicine at 0.1% for 16 hours on seedlings produced 100% tetraploid plants in Martin.
- Taxon
- barleyHordeum vulgaree0
- Material
- Martine5
- Treatment
- 0.1% for 16 hours of seedlingse4
- Outcome
- tetraploid plantse4
- Direction
- increaseinferrede4
- Value
- 100%e5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An experiment was conducted to induce tetraploidy in three diploid barley varieties (Martin, Rihane and Manel) through different colchicines treatments”
e4“However, tetraploid plants were produced only by treatment with 0.1% for 16 hours of seedlings”
e5“The percentages of plants were 40%, 44% and 100% for Rihane, Manel and Martin, respectively”
Claim · model-written summary
Colchicine-treated barley showed chromosome numbers changing from 2n=2x=14 to 2n=4x=28.
- Taxon
- barleyHordeum vulgaree0
- Outcome
- chromosome numberse6
- Value
- from 2n=2x=14 to 2n=4x=28e6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An experiment was conducted to induce tetraploidy in three diploid barley varieties (Martin, Rihane and Manel) through different colchicines treatments”
e6“Cytological analyses showed the increase of chromosome numbers from 2n=2x=14 to 2n=4x=28”
Claim · model-written summary
Increased ploidy significantly decreased plant height, tiller height and leaf number of barley compared to diploid controls.
- Taxon
- barleyHordeum vulgaree0
- Treatment
- increase of ploidy levelse7
- Outcome
- plant height, tiller height, leaf numbere1
- Direction
- decreasee1
- Qualifier
- P<0.01e1
- Comparator
- diploid control plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An experiment was conducted to induce tetraploidy in three diploid barley varieties (Martin, Rihane and Manel) through different colchicines treatments”
e7“The increase of ploidy levels caused major changes in some morphological traits”
e1“the induced tetraploids in barley was accompanied by significant (P<0.01) decrease in plant height, tiller height, leaf number and leaf length compared to diploid control plants”
Claim · model-written summary
Increased ploidy significantly decreased leaf length of barley compared to diploid controls.
- Taxon
- barleyHordeum vulgaree0
- Treatment
- increase of ploidy levelse7
- Outcome
- leaf lengthe1
- Direction
- decreasee1
- Qualifier
- P<0.01e1
- Comparator
- diploid control plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An experiment was conducted to induce tetraploidy in three diploid barley varieties (Martin, Rihane and Manel) through different colchicines treatments”
e7“The increase of ploidy levels caused major changes in some morphological traits”
e1“the induced tetraploids in barley was accompanied by significant (P<0.01) decrease in plant height, tiller height, leaf number and leaf length compared to diploid control plants”
Claim · model-written summary
Colchicine treatment could be used in breeding programs to produce tetraploid barley.
- Taxon
- barleyHordeum vulgaree0
- Outcome
- could be used in breeding programse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“colchicine treatment induce successfully the production of tetraploid barley plants and could be used in breeding programs”
The same questions in other papers
colchicine · growth · Hordeum vulgare (barley)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.