Paper · 2009 · Journal of Xiamen University

Effects of Precursor and Elicitor on Isoflavone Accumulation in Cell-suspension Cultures of Soybean

Hongfang Li

7
claims
13
verbatim quotes
3
citations
1
research areas
—
Not open access

Abstract

The effects of methyl jasmonate,dimethylsulfoxide(DMSO),and phenylalanine on the growth of suspended cells,accumulation of total isoflavones and daidzein in cell-suspension cultures of soybean were studied.Cell-suspension cultures of soybean were established from soybean hypocotyl callus and could accumulated isoflavone stably.The results showed that dimethylsulfoxide and phenylalanine could promote cell growth and dry matter accumulation.The contents of total isoflavone and daidzein in cell-suspension cultures of soybean were greatly increased when 75 mmol/L dimethylsulfoxide was added in the culture system for 48 h,being 1.6 and 1.8 times of the control,respectively.Methyl jasmonate,however,could not contribute to the growth of the suspened cells and elicit the accumulation of total isoflavone and daidzein in cell-suspension cultures of soybean.

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The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 7

Observationobservedoriginal resultmeasured

Claim · model-written summary

Soybean cell-suspension cultures established from hypocotyl callus accumulate isoflavone stably.

Taxon
soybeanGlycine maxe1
Organ / stage
hypocotyl calluse1
Outcome
isoflavonee1
Qualifier
stablye1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e1

“Cell-suspension cultures of soybean were established from soybean hypocotyl callus and could accumulated isoflavone stably”

✓ located verbatim in the source · abstract, characters 206–328 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Dimethylsulfoxide promoted cell growth and dry matter accumulation in soybean cell-suspension cultures.

Taxon
soybeanGlycine maxe1
Treatment
dimethylsulfoxidee2
Outcome
cell growth and dry matter accumulatione2
Direction
promotee2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e1

“Cell-suspension cultures of soybean were established from soybean hypocotyl callus and could accumulated isoflavone stably”

✓ located verbatim in the source · abstract, characters 206–328 · show in text ↑
e2

“The results showed that dimethylsulfoxide and phenylalanine could promote cell growth and dry matter accumulation”

✓ located verbatim in the source · abstract, characters 329–442 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Phenylalanine promoted cell growth and dry matter accumulation in soybean cell-suspension cultures.

Taxon
soybeanGlycine maxe1
Treatment
phenylalaninee2
Outcome
cell growth and dry matter accumulatione2
Direction
promotee2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e1

“Cell-suspension cultures of soybean were established from soybean hypocotyl callus and could accumulated isoflavone stably”

✓ located verbatim in the source · abstract, characters 206–328 · show in text ↑
e2

“The results showed that dimethylsulfoxide and phenylalanine could promote cell growth and dry matter accumulation”

✓ located verbatim in the source · abstract, characters 329–442 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Adding 75 mmol/L dimethylsulfoxide for 48 h increased total isoflavone content in soybean cell-suspension cultures to 1.6 times the control.

Taxon
soybeanGlycine maxe1
Treatment
75 mmol/L dimethylsulfoxidee3
Outcome
total isoflavonee3
Direction
greatly increasede3
Value
1.6e3
Qualifier
48 he3
Comparator
the controle3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e1

“Cell-suspension cultures of soybean were established from soybean hypocotyl callus and could accumulated isoflavone stably”

✓ located verbatim in the source · abstract, characters 206–328 · show in text ↑
e3

“The contents of total isoflavone and daidzein in cell-suspension cultures of soybean were greatly increased when 75 mmol/L dimethylsulfoxide was added in the culture system for 48 h,being 1.6 and 1.8 times of the control,respectively”

✓ located verbatim in the source · abstract, characters 443–676 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Adding 75 mmol/L dimethylsulfoxide for 48 h increased daidzein content in soybean cell-suspension cultures to 1.8 times the control.

Taxon
soybeanGlycine maxe1
Treatment
75 mmol/L dimethylsulfoxidee3
Outcome
daidzeine3
Direction
greatly increasede3
Value
1.8e3
Qualifier
48 he3
Comparator
the controle3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e1

“Cell-suspension cultures of soybean were established from soybean hypocotyl callus and could accumulated isoflavone stably”

✓ located verbatim in the source · abstract, characters 206–328 · show in text ↑
e3

“The contents of total isoflavone and daidzein in cell-suspension cultures of soybean were greatly increased when 75 mmol/L dimethylsulfoxide was added in the culture system for 48 h,being 1.6 and 1.8 times of the control,respectively”

✓ located verbatim in the source · abstract, characters 443–676 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Methyl jasmonate did not promote growth of soybean cell-suspension cultures.

Taxon
soybeanGlycine maxe1
Treatment
Methyl jasmonatee4
Outcome
growth of the suspened cellse4
Direction
could not contribute toe4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e1

“Cell-suspension cultures of soybean were established from soybean hypocotyl callus and could accumulated isoflavone stably”

✓ located verbatim in the source · abstract, characters 206–328 · show in text ↑
e4

“Methyl jasmonate,however,could not contribute to the growth of the suspened cells and elicit the accumulation of total isoflavone and daidzein in cell-suspension cultures of soybean”

✓ located verbatim in the source · abstract, characters 677–858 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Methyl jasmonate did not elicit accumulation of total isoflavone and daidzein in soybean cell-suspension cultures.

Taxon
soybeanGlycine maxe1
Treatment
Methyl jasmonatee4
Outcome
accumulation of total isoflavone and daidzeine4
Direction
could note4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e1

“Cell-suspension cultures of soybean were established from soybean hypocotyl callus and could accumulated isoflavone stably”

✓ located verbatim in the source · abstract, characters 206–328 · show in text ↑
e4

“Methyl jasmonate,however,could not contribute to the growth of the suspened cells and elicit the accumulation of total isoflavone and daidzein in cell-suspension cultures of soybean”

✓ located verbatim in the source · abstract, characters 677–858 · show in text ↑

dimethyl sulfoxide · molecular: metabolites · Glycine max (soybean)Positive and negative results

3 papers: 2 positive, 1 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
See all 3 papers →

(-)-methyl jasmonate · molecular: metabolites · Glycine max (soybean)Positive and negative results

8 papers: 6 positive, 2 negative or no effect

1 paper in the same direction as this paper · 6 in the opposite direction

  1. positive
  2. positive
  3. positive

4 papers more in this group

See all 8 papers →

phenylalanine · yield · Glycine max (soybean)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

dimethyl sulfoxide · yield · Glycine max (soybean)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

(-)-methyl jasmonate · growth · Glycine max (soybean)Results agree

4 papers: 0 positive, 4 negative or no effect

3 papers in the same direction as this paper

  1. negative
  2. negative
  3. negative
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.