Abstract
In order to explore the effect of application of special microbial fertilizer on the rhizosphere microflora in different continuous cropping obstacles soil,the soils with different degrees of continuous cropping obstacles were collected and amended with 2% special microbial fertilizer to be used in the pot experiment for peppers cultivation.The methods of dilution plate count and PCR-DGGE were employed to analysis the rhizosphere microflora.The results obtained were as follows.With the increase of continuous cropping year,the number of rhizospheric bacteria and actinomycetes were decreased while the number of rhizospheric fungi was increased;After fertilization of special microbial fertilizer,the number of microbes had changed obviously,bacterial number increased 78.6%,66.7%,54.5%,fungi number decreased 5.0%,12.5%,7.5%,and actinomycetes number increased 18.2%,9.1%,23.5% in 3 different kinds of continuous cropping soils(with low,medium and high degrees of continuous cropping obstacles),respectively;The PCR-DGGE fingerprinting showed that the richness and diversity index of rhizospheric microorganisms decreased with the increase of the continuous cropping year while the population number and structure of rhizospheric microorganisms could be improved and the diversity index could be increased after fertilization of special microbial fertilizer.It was suggested that the special microbial fertilizer could have a significant effect on improving the population density and population structure of microorganisms in rhizosphere soil,but the improved effect could be different due to the different degrees of continuous cropping obstacles.
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Claims 14
Claim · model-written summary
With increasing continuous cropping years, rhizospheric bacteria and actinomycetes decreased in pepper soil.
- Treatment
- continuous cropping yeare1
- Outcome
- number of rhizospheric bacteria and actinomycetese1
- Direction
- decreasede1
- Setting
- pot experimente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the soils with different degrees of continuous cropping obstacles were collected and amended with 2% special microbial fertilizer to be used in the pot experiment for peppers cultivation”
e1“With the increase of continuous cropping year, the number of rhizospheric bacteria and actinomycetes were decreased while the number of rhizospheric fungi was increased”
Claim · model-written summary
With increasing continuous cropping years, rhizospheric fungi increased in pepper soil.
- Treatment
- continuous cropping yeare1
- Outcome
- number of rhizospheric fungie1
- Direction
- increasede1
- Setting
- pot experimente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the soils with different degrees of continuous cropping obstacles were collected and amended with 2% special microbial fertilizer to be used in the pot experiment for peppers cultivation”
e1“With the increase of continuous cropping year, the number of rhizospheric bacteria and actinomycetes were decreased while the number of rhizospheric fungi was increased”
Claim · model-written summary
Special microbial fertilizer increased bacterial number by 78.6% in the low continuous-cropping-obstacle soil of pepper.
- Treatment
- special microbial fertilizere0
- Outcome
- bacterial numbere2
- Direction
- increasede2
- Value
- 78.6%e2
- Setting
- pot experimente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the soils with different degrees of continuous cropping obstacles were collected and amended with 2% special microbial fertilizer to be used in the pot experiment for peppers cultivation”
e2“bacterial number increased 78.6%,66.7%,54.5%, fungi number decreased 5.0%,12.5%,7.5%, and actinomycetes number increased 18.2%,9.1%,23.5% in 3 different kinds of continuous cropping soils”
Claim · model-written summary
Special microbial fertilizer increased bacterial number by 66.7% in the medium continuous-cropping-obstacle soil of pepper.
- Treatment
- special microbial fertilizere0
- Outcome
- bacterial numbere2
- Direction
- increasede2
- Value
- 66.7%e2
- Setting
- pot experimente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the soils with different degrees of continuous cropping obstacles were collected and amended with 2% special microbial fertilizer to be used in the pot experiment for peppers cultivation”
e2“bacterial number increased 78.6%,66.7%,54.5%, fungi number decreased 5.0%,12.5%,7.5%, and actinomycetes number increased 18.2%,9.1%,23.5% in 3 different kinds of continuous cropping soils”
Claim · model-written summary
Special microbial fertilizer increased bacterial number by 54.5% in the high continuous-cropping-obstacle soil of pepper.
- Treatment
- special microbial fertilizere0
- Outcome
- bacterial numbere2
- Direction
- increasede2
- Value
- 54.5%e2
- Setting
- pot experimente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the soils with different degrees of continuous cropping obstacles were collected and amended with 2% special microbial fertilizer to be used in the pot experiment for peppers cultivation”
e2“bacterial number increased 78.6%,66.7%,54.5%, fungi number decreased 5.0%,12.5%,7.5%, and actinomycetes number increased 18.2%,9.1%,23.5% in 3 different kinds of continuous cropping soils”
Claim · model-written summary
Special microbial fertilizer decreased fungal number by 5.0% in the low continuous-cropping-obstacle soil of pepper.
- Treatment
- special microbial fertilizere0
- Outcome
- fungi numbere2
- Direction
- decreasede2
- Value
- 5.0%e2
- Setting
- pot experimente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the soils with different degrees of continuous cropping obstacles were collected and amended with 2% special microbial fertilizer to be used in the pot experiment for peppers cultivation”
e2“bacterial number increased 78.6%,66.7%,54.5%, fungi number decreased 5.0%,12.5%,7.5%, and actinomycetes number increased 18.2%,9.1%,23.5% in 3 different kinds of continuous cropping soils”
Claim · model-written summary
Special microbial fertilizer decreased fungal number by 12.5% in the medium continuous-cropping-obstacle soil of pepper.
- Treatment
- special microbial fertilizere0
- Outcome
- fungi numbere2
- Direction
- decreasede2
- Value
- 12.5%e2
- Setting
- pot experimente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the soils with different degrees of continuous cropping obstacles were collected and amended with 2% special microbial fertilizer to be used in the pot experiment for peppers cultivation”
e2“bacterial number increased 78.6%,66.7%,54.5%, fungi number decreased 5.0%,12.5%,7.5%, and actinomycetes number increased 18.2%,9.1%,23.5% in 3 different kinds of continuous cropping soils”
Claim · model-written summary
Special microbial fertilizer decreased fungal number by 7.5% in the high continuous-cropping-obstacle soil of pepper.
- Treatment
- special microbial fertilizere0
- Outcome
- fungi numbere2
- Direction
- decreasede2
- Value
- 7.5%e2
- Setting
- pot experimente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the soils with different degrees of continuous cropping obstacles were collected and amended with 2% special microbial fertilizer to be used in the pot experiment for peppers cultivation”
e2“bacterial number increased 78.6%,66.7%,54.5%, fungi number decreased 5.0%,12.5%,7.5%, and actinomycetes number increased 18.2%,9.1%,23.5% in 3 different kinds of continuous cropping soils”
Claim · model-written summary
Special microbial fertilizer increased actinomycetes number by 18.2% in the low continuous-cropping-obstacle soil of pepper.
- Treatment
- special microbial fertilizere0
- Outcome
- actinomycetes numbere2
- Direction
- increasede2
- Value
- 18.2%e2
- Setting
- pot experimente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the soils with different degrees of continuous cropping obstacles were collected and amended with 2% special microbial fertilizer to be used in the pot experiment for peppers cultivation”
e2“bacterial number increased 78.6%,66.7%,54.5%, fungi number decreased 5.0%,12.5%,7.5%, and actinomycetes number increased 18.2%,9.1%,23.5% in 3 different kinds of continuous cropping soils”
Claim · model-written summary
Special microbial fertilizer increased actinomycetes number by 9.1% in the medium continuous-cropping-obstacle soil of pepper.
- Treatment
- special microbial fertilizere0
- Outcome
- actinomycetes numbere2
- Direction
- increasede2
- Value
- 9.1%e2
- Setting
- pot experimente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the soils with different degrees of continuous cropping obstacles were collected and amended with 2% special microbial fertilizer to be used in the pot experiment for peppers cultivation”
e2“bacterial number increased 78.6%,66.7%,54.5%, fungi number decreased 5.0%,12.5%,7.5%, and actinomycetes number increased 18.2%,9.1%,23.5% in 3 different kinds of continuous cropping soils”
Claim · model-written summary
Special microbial fertilizer increased actinomycetes number by 23.5% in the high continuous-cropping-obstacle soil of pepper.
- Treatment
- special microbial fertilizere0
- Outcome
- actinomycetes numbere2
- Direction
- increasede2
- Value
- 23.5%e2
- Setting
- pot experimente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the soils with different degrees of continuous cropping obstacles were collected and amended with 2% special microbial fertilizer to be used in the pot experiment for peppers cultivation”
e2“bacterial number increased 78.6%,66.7%,54.5%, fungi number decreased 5.0%,12.5%,7.5%, and actinomycetes number increased 18.2%,9.1%,23.5% in 3 different kinds of continuous cropping soils”
Claim · model-written summary
With increasing continuous cropping years, richness and diversity index of rhizospheric microorganisms of pepper decreased.
- Treatment
- continuous cropping yeare3
- Outcome
- richness and diversity index of rhizospheric microorganismse3
- Direction
- decreasede3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the soils with different degrees of continuous cropping obstacles were collected and amended with 2% special microbial fertilizer to be used in the pot experiment for peppers cultivation”
e3“the richness and diversity index of rhizospheric microorganisms decreased with the increase of the continuous cropping year”
Claim · model-written summary
Special microbial fertilizer could increase the diversity index of rhizospheric microorganisms of pepper.
- Treatment
- special microbial fertilizere4
- Outcome
- diversity indexe4
- Direction
- could be increasede4
- Setting
- pot experimente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the soils with different degrees of continuous cropping obstacles were collected and amended with 2% special microbial fertilizer to be used in the pot experiment for peppers cultivation”
e4“the population number and structure of rhizospheric microorganisms could be improved and the diversity index could be increased after fertilization of special microbial fertilizer”
Claim · model-written summary
Special microbial fertilizer could improve the population number and structure of rhizospheric microorganisms of pepper.
- Treatment
- special microbial fertilizere4
- Outcome
- population number and structure of rhizospheric microorganismse4
- Direction
- could be improvede4
- Setting
- pot experimente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the soils with different degrees of continuous cropping obstacles were collected and amended with 2% special microbial fertilizer to be used in the pot experiment for peppers cultivation”
e4“the population number and structure of rhizospheric microorganisms could be improved and the diversity index could be increased after fertilization of special microbial fertilizer”
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