Abstract
Immature embryos and embryogenic calli of 16 indica rice cultivars have been transformed usingparticle bombardment. We set up a variety of parameters to optimize the important factors involved in biolistictransformation of indica rice, and supplement various hormones , mannitol and MES to NB medium in order to improve growth state of subcultured calli. Results indicate that operation parameters (include concentration of AuDNA complex, helium pressure, and receptor state) involved in microprojectile-mediated transformat ion are veryimportant to transformation rate; MES (500 mg/L) added to the subculture medium as a buffer can mitigatebrowning of indica rice calli; supplementing NAA(0. 5 mg/L),KT(0. 2 mg/L) to the induce medium and the subculture medium can improve the quality of calli and increase the regeneration rate ; ABA(1 mg/L) and mannitol (20g/L) can improve the growth state of calli, mitigate browning and obtain more granular Basta calli that are fineand close in texture. The transgenic plants resistant to Basta were obtained.
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Claims 8
Claim · model-written summary
Bombardment operating parameters were important to transformation rate.
- Taxon
- indica rice
Oryza sativa Indica Groupt0 - Outcome
- transformation ratee0
- Qualifier
- concentration of AuDNA complex, helium pressure, and receptor statee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“operation parameters (include concentration of AuDNA complex, helium pressure, and receptor state) involved in microprojectile-mediated transformat ion are veryimportant to transformation rate”
t0“Indica Rice”
Claim · model-written summary
MES buffer mitigated browning of indica rice calli.
- Taxon
- indica riceOryza sativa Indica Groupe0
- Treatment
- MES (500 mg/L)e0
- Outcome
- browning of indica rice callie0
- Direction
- mitigatebrowninge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MES (500 mg/L) added to the subculture medium as a buffer can mitigatebrowning of indica rice calli”
Claim · model-written summary
NAA plus KT improved callus quality.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Treatment
- NAA(0. 5 mg/L),KT(0. 2 mg/L)e0
- Outcome
- quality of callie0
- Direction
- improvee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“supplementing NAA(0. 5 mg/L),KT(0. 2 mg/L) to the induce medium and the subculture medium can improve the quality of calli and increase the regeneration rate”
t0“Indica Rice”
Claim · model-written summary
NAA plus KT increased callus regeneration rate.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Treatment
- NAA(0. 5 mg/L),KT(0. 2 mg/L)e0
- Outcome
- regeneration ratee0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“supplementing NAA(0. 5 mg/L),KT(0. 2 mg/L) to the induce medium and the subculture medium can improve the quality of calli and increase the regeneration rate”
t0“Indica Rice”
Claim · model-written summary
ABA plus mannitol improved callus growth state.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Treatment
- ABA(1 mg/L) and mannitol (20g/L)e0
- Outcome
- growth state of callie0
- Direction
- improvee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA(1 mg/L) and mannitol (20g/L) can improve the growth state of calli, mitigate browning and obtain more granular Basta calli that are fineand close in texture.”
t0“Indica Rice”
Claim · model-written summary
ABA plus mannitol mitigated callus browning.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Treatment
- ABA(1 mg/L) and mannitol (20g/L)e0
- Outcome
- browninge0
- Direction
- mitigatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA(1 mg/L) and mannitol (20g/L) can improve the growth state of calli, mitigate browning and obtain more granular Basta calli that are fineand close in texture.”
t0“Indica Rice”
Claim · model-written summary
ABA plus mannitol yielded more granular, fine and close-textured Basta calli.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Treatment
- ABA(1 mg/L) and mannitol (20g/L)e0
- Outcome
- more granular Basta calli that are fineand close in texturee0
- Direction
- moree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA(1 mg/L) and mannitol (20g/L) can improve the growth state of calli, mitigate browning and obtain more granular Basta calli that are fineand close in texture.”
t0“Indica Rice”
Claim · model-written summary
Basta-resistant transgenic plants were obtained.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Outcome
- transgenic plants resistant to Basta were obtainede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transgenic plants resistant to Basta were obtained.”
t0“Indica Rice”
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8 of 9 groups shown: named compounds and organisms first, then groups whose results differ (checked claim by claim), then those with positive and negative results, then the smaller groups.
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.