Abstract
This paper deals with the effects of hormone combinations on protoplast culture of cucumber (Cucumis sativus L. cv. ‘Ohgonsokusei’). Protoplast isolated enzymatically from cotyledon divided over a wide range of the hormone combinations tested here. The media containing 0.5-2.5mg/l NAA+0.5-2.5mg/l BA and 0.5mg/l NAA+0.1mg/l 2ip were particularly suitable for callus and root formation from protoplast-derived colonies, respectively. Various combinations of hormones have been tried for inducing plant regeneration from callus culture, but the attempt has so far been unsuccessful.
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Claims 4
Claim · model-written summary
Cotyledon protoplasts divided over a wide range of hormone combinations.
- Taxon
- cucumber
Cucumis sativuse0 - Material
- ‘Ohgonsokusei’e0
- Organ / stage
- cotyledone0
- Outcome
- divided over a wide range of the hormone combinationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“protoplast culture of cucumber (Cucumis sativus L. cv. ‘Ohgonsokusei’). Protoplast isolated enzymatically from cotyledon divided over a wide range of the hormone combinations tested here.”
Claim · model-written summary
NAA plus BA media were particularly suitable for callus formation.
- Taxon
- cucumberCucumis sativust0
- Treatment
- 0.5-2.5mg/l NAA+0.5-2.5mg/l BAe0
- Outcome
- calluse0
- Direction
- particularly suitablee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The media containing 0.5-2.5mg/l NAA+0.5-2.5mg/l BA and 0.5mg/l NAA+0.1mg/l 2ip were particularly suitable for callus and root formation from protoplast-derived colonies, respectively.”
t0“cucumber (Cucumis sativus L.)”
Claim · model-written summary
NAA plus 2ip medium was particularly suitable for root formation.
- Taxon
- cucumberCucumis sativust0
- Treatment
- 0.5mg/l NAA+0.1mg/l 2ipe0
- Outcome
- root formatione0
- Direction
- particularly suitablee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The media containing 0.5-2.5mg/l NAA+0.5-2.5mg/l BA and 0.5mg/l NAA+0.1mg/l 2ip were particularly suitable for callus and root formation from protoplast-derived colonies, respectively.”
t0“cucumber (Cucumis sativus L.)”
Claim · model-written summary
The tested hormone combinations did not regenerate plants from callus.
- Taxon
- cucumberCucumis sativust0
- Treatment
- Various combinations of hormonese0
- Outcome
- plant regeneration from callus culturee0
- Direction
- unsuccessfule0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Various combinations of hormones have been tried for inducing plant regeneration from callus culture, but the attempt has so far been unsuccessful.”
t0“cucumber (Cucumis sativus L.)”
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