By organism › Bacteria › Rhizobiaceae › Agrobacterium tumefaciens

Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Histidine-auxotrophic EHA105hisD− was generated by gene deletion.

Taxon: Agrobacterium tumefaciensAgrobacterium tumefacienst0
Outcome: EHA105hisD−e0
Comparator: EHA105e0

Verbatim

e0
“generated novel histidine and leucine auxotrophic strains of A. tumefaciens EHA105, namely EHA105hisD− and EHA105leuA−, as well as a dual auxotrophic strain EHA105hisD−leuA−, through gene deletion”
✓ located verbatim in the source · abstract, characters 201–397

t0
“Agrobacterium tumefaciens Strains for Transformation”
✓ located verbatim in the source · title, characters 35–87

Title

Effective Use of Novel Auxotrophic Agrobacterium tumefaciens Strains for Transformation and Biocontainment

Lichi Zhong; Huiting Guo; Ling Wu; et al. Plants 2025. doi:10.3390/plants14060925

Other claims from this paper (7)

Method / resource Leucine-auxotrophic EHA105leuA− was generated by gene deletion.
Method / resource Dual auxotrophic EHA105hisD−leuA− was generated by gene deletion.
Method / resource Transient expression efficiency of all three strains in Nicotiana was significantly impaired but restored with amino acids.
Method / resource Survival rate of all three strains in Nicotiana was significantly impaired but restored with amino acids.
Method / resource With amino acid supplementation, transformation using auxotrophic strains significantly reduced Agrobacterium overgrowth.
Method / resource With amino acid supplementation, transformation efficiency matched wild-type EHA105.
Method / resource Auxotrophic strain cell survival on plants and soil was much lower than wild-type EHA105.

Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Leucine-auxotrophic EHA105leuA− was generated by gene deletion.

Taxon: Agrobacterium tumefaciensAgrobacterium tumefacienst0
Outcome: EHA105leuA−e0
Comparator: EHA105e0

Verbatim

e0
“generated novel histidine and leucine auxotrophic strains of A. tumefaciens EHA105, namely EHA105hisD− and EHA105leuA−, as well as a dual auxotrophic strain EHA105hisD−leuA−, through gene deletion”
✓ located verbatim in the source · abstract, characters 201–397

t0
“Agrobacterium tumefaciens Strains for Transformation”
✓ located verbatim in the source · title, characters 35–87

Title

Effective Use of Novel Auxotrophic Agrobacterium tumefaciens Strains for Transformation and Biocontainment

Lichi Zhong; Huiting Guo; Ling Wu; et al. Plants 2025. doi:10.3390/plants14060925

Other claims from this paper (7)

Method / resource Histidine-auxotrophic EHA105hisD− was generated by gene deletion.
Method / resource Dual auxotrophic EHA105hisD−leuA− was generated by gene deletion.
Method / resource Transient expression efficiency of all three strains in Nicotiana was significantly impaired but restored with amino acids.
Method / resource Survival rate of all three strains in Nicotiana was significantly impaired but restored with amino acids.
Method / resource With amino acid supplementation, transformation using auxotrophic strains significantly reduced Agrobacterium overgrowth.
Method / resource With amino acid supplementation, transformation efficiency matched wild-type EHA105.
Method / resource Auxotrophic strain cell survival on plants and soil was much lower than wild-type EHA105.

Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Dual auxotrophic EHA105hisD−leuA− was generated by gene deletion.

Taxon: Agrobacterium tumefaciensAgrobacterium tumefacienst0
Outcome: EHA105hisD−leuA−e0
Comparator: Agrobacterium tumefacienst0

Verbatim

e0
“a dual auxotrophic strain EHA105hisD−leuA−, through gene deletion”
✓ located verbatim in the source · abstract, characters 332–397

t0
“Agrobacterium tumefaciens Strains for Transformation”
✓ located verbatim in the source · title, characters 35–87

Title

Effective Use of Novel Auxotrophic Agrobacterium tumefaciens Strains for Transformation and Biocontainment

Lichi Zhong; Huiting Guo; Ling Wu; et al. Plants 2025. doi:10.3390/plants14060925

Other claims from this paper (7)

Method / resource Histidine-auxotrophic EHA105hisD− was generated by gene deletion.
Method / resource Leucine-auxotrophic EHA105leuA− was generated by gene deletion.
Method / resource Transient expression efficiency of all three strains in Nicotiana was significantly impaired but restored with amino acids.
Method / resource Survival rate of all three strains in Nicotiana was significantly impaired but restored with amino acids.
Method / resource With amino acid supplementation, transformation using auxotrophic strains significantly reduced Agrobacterium overgrowth.
Method / resource With amino acid supplementation, transformation efficiency matched wild-type EHA105.
Method / resource Auxotrophic strain cell survival on plants and soil was much lower than wild-type EHA105.

Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Loss of antimicrobial compound production reduced FZB42 mutant inhibition of A. tumefaciens XYT-58.

Taxon: A. tumefaciens XYT-58Agrobacterium tumefaciense0
Treatment: B. velezensis FZB42 mutants unable to produce one or a combination of four antimicrobial compoundse0
Interactor: B. velezensis FZB42Bacillus velezensise0biocontrol_agent
Outcome: inhibitory activities against A. tumefaciens XYT-58e0
Direction: reducede0

Not reportedComparator

Verbatim

e0
“B. velezensis FZB42 mutants unable to produce one or a combination of four antimicrobial compounds (surfactin, macrolactin, bacilysin, and difficidin) significantly reduced their inhibitory activities against A. tumefaciens XYT-58”
✓ located verbatim in the source · abstract, characters 454–684

Title

Four Antimicrobial Compounds and ISR Induction are Involved in Biocontrol of Crown Gall Disease by the Plant Beneficial Rhizobacterium Bacillus velezensis FZB42

Xu Qin; Yueting Xiao; Qin Xiong; et al. Plant Disease 2025. doi:10.1094/pdis-10-24-2085-re

Other claims from this paper (7)

Intervention effect Those FZB42 mutants had reduced ability to control crown gall in cherry.
Intervention effect Purified bacilysin inhibited XYT-58 growth in vitro.
Intervention effect Purified surfactin inhibited XYT-58 growth in vitro.
Intervention effect FZB42 inoculation increased expression of jasmonic acid, ethylene and salicylic acid pathway genes in infected cherry seedlings.
Intervention effect Bacilysin suppressed Agrobacterium growth.
Intervention effect Difficidin suppressed Agrobacterium growth.
Mechanism Multiple antimicrobial compounds and induced systemic resistance contribute to FZB42 control of crown gall.

Observationobservedoriginal resultmeasured

Claim · model-written summary

Growth on the listed selective media produced positive diagnostic results.

Taxon: Agrobacterium tumefaciensAgrobacterium tumefacienst0
Outcome: positive resultse0

Not reportedDirection

Verbatim

e0
“Growth on the following selective media for their diagnosis produced positive results ; Mac C onkey agar, D1 medium, Luria- B ertani medium, glucose peptone agar, potato dextrose agar, AMM, chromogenic agar medium.”
✓ located verbatim in the source · abstract, characters 1015–1229

t0
“Agrobacterium tumefaciens”
✓ located verbatim in the source · title, characters 37–62

Title

Genetic diagnosis of phytopathogenic Agrobacterium tumefaciens isolates from Cydonia oblonga trees by PCR using 16SrRNA primers

A. Q. S. Khdeer; R. N. Gergees Regulatory Mechanisms in Biosystems 2025. doi:10.15421/0225005

Other claims from this paper (3)

Observation Five isolates formed tumors on carrot discs over 22 to 26 days.
Method / resource PCR produced single bands of 1150 bp in five isolates, supporting their genus identification.
Method / resource Biovar I isolates AtCo5 and AtCo2 from quince tumors were deposited under accession PQ106659.

Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

PCR produced single bands of 1150 bp in five isolates, supporting their genus identification.

Taxon: Agrobacterium tumefaciensAgrobacterium tumefacienst0
Outcome: single, shiny bandse0
Value: 1150 bpe0

Not reportedDirection

Verbatim

e0
“This allowed the clear separ a tion of single, shiny bands at the molecular size (1150 bp) in the five isolates, confirming their membership of the genus Agrobact e rium .”
✓ located verbatim in the source · abstract, characters 1931–2102

t0
“Agrobacterium tumefaciens”
✓ located verbatim in the source · title, characters 37–62

Title

Genetic diagnosis of phytopathogenic Agrobacterium tumefaciens isolates from Cydonia oblonga trees by PCR using 16SrRNA primers

A. Q. S. Khdeer; R. N. Gergees Regulatory Mechanisms in Biosystems 2025. doi:10.15421/0225005

Other claims from this paper (3)

Observation Five isolates formed tumors on carrot discs over 22 to 26 days.
Observation Growth on the listed selective media produced positive diagnostic results.
Method / resource Biovar I isolates AtCo5 and AtCo2 from quince tumors were deposited under accession PQ106659.

Method / resourceobservedoriginal resultbasis n/a

Claim · model-written summary

Biovar I isolates AtCo5 and AtCo2 from quince tumors were deposited under accession PQ106659.

Taxon: Agrobacterium tumefaciensAgrobacterium tumefacienst0
Material: AtCo5 and AtCo2e0
Outcome: listed in the gene banke0
Value: PQ106659e0

Verbatim

e0
“Both of the Biovar I isolates (AtCo5 and AtCo2) from quince tumors were listed in the gene bank unde r the accession nu m ber PQ106659.”
✓ located verbatim in the source · abstract, characters 2103–2238

t0
“Agrobacterium tumefaciens”
✓ located verbatim in the source · title, characters 37–62

Title

Genetic diagnosis of phytopathogenic Agrobacterium tumefaciens isolates from Cydonia oblonga trees by PCR using 16SrRNA primers

A. Q. S. Khdeer; R. N. Gergees Regulatory Mechanisms in Biosystems 2025. doi:10.15421/0225005

Other claims from this paper (3)

Observation Five isolates formed tumors on carrot discs over 22 to 26 days.
Observation Growth on the listed selective media produced positive diagnostic results.
Method / resource PCR produced single bands of 1150 bp in five isolates, supporting their genus identification.

Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Tea leaves induced significant morphological aberrations in bacterial cells.

Taxon: Agrobacterium tumefaciensAgrobacterium tumefaciense1
Treatment: tea leavese0
Interactor: teae0host
Outcome: morphological aberrations on bacterial cellse0
Comparator: Nicotiana benthamiana leavese1

Not reportedDirection

Verbatim

e0
“tea leaves induced significant morphological aberrations on bacterial cells and affected pathogen–plant attachment”
✓ located verbatim in the source · abstract, characters 511–625

e1
“the morphology, growth, and gene expression of Agrobacterium tumefaciens during tea-leaf explant infection, were compared to AMT of Nicotiana benthamiana leaves”
✓ located verbatim in the source · abstract, characters 275–435

Title

Comparative Transcriptome Analysis of Agrobacterium tumefaciens Reveals the Molecular Basis for the Recalcitrant Genetic Transformation of Camellia sinensis L.

Ke Jin; Na Tian; Jorge Freire da Silva Ferreira; et al. Biomolecules 2022. doi:10.3390/biom12050688

Other claims from this paper (7)

Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

The tea-leaf phylloplane severely affected expression of bacterial metabolic, ABC transporter and two-component-system genes.

Taxon: Agrobacterium tumefaciensAgrobacterium tumefacienst0
Treatment: tea-leaf phylloplanee0
Interactor: teae0host
Outcome: expressions of genese0
Qualifier: bacterial fundamental metabolic processes, ATP-binding cassette (ABC) transporters, two-component systems (TCSs)e0

Not reportedComparatorDirection

Verbatim

e0
“The expressions of genes involved in bacterial fundamental metabolic processes, ATP-binding cassette (ABC) transporters, two-component systems (TCSs), secretion systems, and quorum sensing (QS) systems were severely affected in response to the tea-leaf phylloplane.”
✓ located verbatim in the source · abstract, characters 900–1165

t0
“Agrobacterium tumefaciens”
✓ located verbatim in the source · title, characters 38–63

Title

Comparative Transcriptome Analysis of Agrobacterium tumefaciens Reveals the Molecular Basis for the Recalcitrant Genetic Transformation of Camellia sinensis L.

Ke Jin; Na Tian; Jorge Freire da Silva Ferreira; et al. Biomolecules 2022. doi:10.3390/biom12050688

Other claims from this paper (7)

Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

The tea-leaf phylloplane severely affected expression of bacterial secretion and quorum-sensing genes.

Taxon: Agrobacterium tumefaciensAgrobacterium tumefacienst0
Treatment: tea-leaf phylloplanee0
Interactor: teae0host
Outcome: expressions of genese0
Qualifier: secretion systems, and quorum sensing (QS) systemse0

Not reportedComparatorDirection

Verbatim

e0
“The expressions of genes involved in bacterial fundamental metabolic processes, ATP-binding cassette (ABC) transporters, two-component systems (TCSs), secretion systems, and quorum sensing (QS) systems were severely affected in response to the tea-leaf phylloplane.”
✓ located verbatim in the source · abstract, characters 900–1165

t0
“Agrobacterium tumefaciens”
✓ located verbatim in the source · title, characters 38–63

Title

Comparative Transcriptome Analysis of Agrobacterium tumefaciens Reveals the Molecular Basis for the Recalcitrant Genetic Transformation of Camellia sinensis L.

Ke Jin; Na Tian; Jorge Freire da Silva Ferreira; et al. Biomolecules 2022. doi:10.3390/biom12050688

Other claims from this paper (7)

Agrobacterium tumefaciens 144 claims

144 claims