Abstract
Micropropagation of guava cv. Allahabad Safeda was achieved on nodal explant from field grown mature trees. The nodal explants excised during April-June period were found better for culture initiation. Multiple surface sterilizing agents (H 2 O 2 , AgNO 3 and HgCl 2 ) were used for getting pure cultures. The problem of phenolic browning was minimized to a great extent by agitation of explants in PVPP (0.5%) and sucrose (2%) for 40 min. followed by quick dip in antioxidant solution (citric acid and ascorbic acid) prior to inoculation. The explant bud sprouting was not significantly influenced by various basal media supplemented with 1 mg l -1 BAP. The shoot multiplication was better on WPM with 1 mg l -1 BAP and incorporation of IBA could not increase the shoot proliferation rate. The best rooting response was obtained on WPM supplemented with 0.2 mg l -1 each of IBA & NAA and 200 mg l -1 activated charcoal. The regenerated plantlets were established on peat and soilrite based rooting mixture for hardening in the glass house. Finally 50% of the plantlets survived on field transfer.
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Claims 7
Claim · model-written summary
Nodal explants collected in April-June were better for culture initiation.
- Taxon
- guava
Psidium guajavae0 - Material
- Allahabad Safedae0
- Organ / stage
- nodal explantse1
- Treatment
- April-June periode1
- Outcome
- culture initiatione1
- Direction
- bettere1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“guava cv. Allahabad Safeda”
e1“nodal explants excised during April-June period were found better for culture initiation”
Claim · model-written summary
PVPP and sucrose agitation followed by antioxidant dip minimized phenolic browning.
- Taxon
- guavaPsidium guajavae0
- Treatment
- agitation of explants in PVPP (0.5%) and sucrose (2%) for 40 min. followed by quick dip in antioxidant solution (citric acid and ascorbic acid)e1
- Outcome
- phenolic browninge1
- Direction
- minimizede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“guava cv. Allahabad Safeda”
e1“phenolic browning was minimized to a great extent by agitation of explants in PVPP (0.5%) and sucrose (2%) for 40 min. followed by quick dip in antioxidant solution (citric acid and ascorbic acid)”
Claim · model-written summary
Basal media with BAP did not significantly influence bud sprouting.
- Taxon
- guavaPsidium guajavae0
- Treatment
- various basal media supplemented with 1 mg l -1 BAPe1
- Outcome
- explant bud sproutinge1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“guava cv. Allahabad Safeda”
e1“explant bud sprouting was not significantly influenced by various basal media supplemented with 1 mg l -1 BAP”
Claim · model-written summary
Shoot multiplication was better on WPM with BAP.
- Taxon
- guavaPsidium guajavae0
- Treatment
- WPM with 1 mg l -1 BAPe1
- Outcome
- shoot multiplicatione1
- Direction
- bettere1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“guava cv. Allahabad Safeda”
e1“shoot multiplication was better on WPM with 1 mg l -1 BAP”
Claim · model-written summary
IBA did not increase shoot proliferation rate.
- Taxon
- guavaPsidium guajavae0
- Treatment
- incorporation of IBAe1
- Outcome
- shoot proliferation ratee1
- Direction
- could not increasee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“guava cv. Allahabad Safeda”
e1“incorporation of IBA could not increase the shoot proliferation rate”
Claim · model-written summary
WPM with IBA, NAA and charcoal gave the best rooting response.
- Taxon
- guavaPsidium guajavae0
- Treatment
- WPM supplemented with 0.2 mg l -1 each of IBA & NAA and 200 mg l -1 activated charcoale1
- Outcome
- rooting responsee1
- Direction
- beste1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“guava cv. Allahabad Safeda”
e1“best rooting response was obtained on WPM supplemented with 0.2 mg l -1 each of IBA & NAA and 200 mg l -1 activated charcoal”
Claim · model-written summary
Half of the regenerated plantlets survived field transfer.
- Taxon
- guavaPsidium guajavae0
- Organ / stage
- plantletse1
- Outcome
- plantlets survived on field transfere1
- Value
- 50%e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“guava cv. Allahabad Safeda”
e1“50% of the plantlets survived on field transfer”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.