Paper · 2000 · Journal of Applied Horticulture

Effect of surface sterilizing agents on in vitro culture establishment of guava (Psidium guajava L.).

P.R. Meghwal; H.C. Sharma; S.K. Singh

3
claims
6
verbatim quotes
15
citations
3
research areas
—
Not open access

Abstract

A method was standardized for the quick establishment of aseptic cultures in guava from mature field-grown stock plants for micropropagation through enhanced axillary branching technique. The maximum number of aseptic explants with shoot proliferation was obtained by a combination of surface sterilizing agents involving hydrogen peroxide (10%), silver nitrate (0.25%), and mercuric chloride (0.05%) treatment of explants one by one for five, six, and three minutes, respectively. The problem of phenolic browning was also minimized to a great extent by leaching of phenolic compounds due to agitation in antioxidant solution as well as by proper drying of explant prior to inoculation.

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Claims 3

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Sequential sterilization maximized aseptic explants with shoot proliferation.

Organ / stage
explantse0
Treatment
hydrogen peroxide (10%), silver nitrate (0.25%), and mercuric chloride (0.05%) treatmente0
Outcome
aseptic explants with shoot proliferatione0
Direction
maximume0
Qualifier
one by one for five, six, and three minutes, respectivelye0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Psidium guajava
Psidium guajava · TaxonFuadsyazwan ( talk ) · Public domain · Wikimedia Commons ↗

Verbatim

t0

“guava (Psidium guajava L.)”

✓ located verbatim in the source · title, characters 74–100 · show in text ↑
e0

“The maximum number of aseptic explants with shoot proliferation was obtained by a combination of surface sterilizing agents involving hydrogen peroxide (10%), silver nitrate (0.25%), and mercuric chloride (0.05%) treatment of explants one by one for five, six, and three minutes, respectively.”

✓ located verbatim in the source · abstract, characters 188–481 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Agitation in antioxidant solution minimized phenolic browning.

Treatment
agitation in antioxidant solutione0
Outcome
phenolic browninge0
Direction
minimizede0
Qualifier
to a great extente0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Psidium guajava
Psidium guajava · TaxonFuadsyazwan ( talk ) · Public domain · Wikimedia Commons ↗

Verbatim

t0

“guava (Psidium guajava L.)”

✓ located verbatim in the source · title, characters 74–100 · show in text ↑
e0

“The problem of phenolic browning was also minimized to a great extent by leaching of phenolic compounds due to agitation in antioxidant solution as well as by proper drying of explant prior to inoculation.”

✓ located verbatim in the source · abstract, characters 482–687 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Drying explants before inoculation minimized phenolic browning.

Organ / stage
explante0
Treatment
proper drying of explant prior to inoculatione0
Outcome
phenolic browninge0
Direction
minimizede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Psidium guajava
Psidium guajava · TaxonFuadsyazwan ( talk ) · Public domain · Wikimedia Commons ↗

Verbatim

t0

“guava (Psidium guajava L.)”

✓ located verbatim in the source · title, characters 74–100 · show in text ↑
e0

“The problem of phenolic browning was also minimized to a great extent by leaching of phenolic compounds due to agitation in antioxidant solution as well as by proper drying of explant prior to inoculation.”

✓ located verbatim in the source · abstract, characters 482–687 · show in text ↑

antioxidant · disease · Psidium guajava (guava)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

hydrogen peroxide · growth · Psidium guajava (guava)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

processing · disease · Psidium guajava (guava)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. negative
See all 2 papers →

inoculation · disease · Psidium guajava (guava)Positive and negative results

8 papers: 2 positive, 6 negative or no effect

1 paper in the same direction as this paper · 6 in the opposite direction

  1. negative
  2. no effect
  3. negative

4 papers more in this group

See all 8 papers →

in vitro · disease · Psidium guajava (guava)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.