Abstract
plant is widely adopted and can tolerate frost, drought and salinity conditions. In the present study, a rapid, simple and efficient protocol for in vitro propagation of guava (P. guajava L.) from nodal segments of adult trees grown in the field was established. Explants collected in Autumn and cultured on Murashige and Skoog (MS) medium supplemented with 6-benzyl adenine (BA) at 8.9 M, plus indole-3-butyric acid (IBA) at 0.98 M showed the best response in the in vitro establishment (91.7% sprouting percentage and shoot length of 1.75 cm). In addition, 100 mg/l ascorbic acid and 150 mg/l citric acid as antioxidants and Polyvinylpyrrolidone (PVP) at 100 mg/l and activated charcoal (AC) at 2 g/l as absorbents were added to the establishment medium to reduce phenolic compounds. Regarding average number and length of shoots per explant, BA was more effective than kinetin (kin). Maximum rooting percentage (66.7%) occurred on solid half-strength MS medium containing indol-3-butyric acid (IBA) at 9.8 M. Fifty percent (50%) rooted plantlets were successfully acclimatized in the greenhouse in soil mixture of sand, grand soil and peat moss at equal volumes, and subsequently established outside the greenhouse.
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Claims 5
Claim · model-written summary
Autumn explants on BA and IBA medium gave the best establishment response, with 91.7% sprouting.
- Taxon
- guava
Psidium guajavae1 - Organ / stage
- Explantse0
- Treatment
- Murashige and Skoog (MS) medium supplemented with 6-benzyl adenine (BA) at 8.9 M, plus indole-3-butyric acid (IBA) at 0.98 Me0
- Outcome
- sprouting percentagee0
- Direction
- beste0
- Value
- 91.7%e0
- Qualifier
- Autumne0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Explants collected in Autumn and cultured on Murashige and Skoog (MS) medium supplemented with 6-benzyl adenine (BA) at 8.9 M, plus indole-3-butyric acid (IBA) at 0.98 M showed the best response in the in vitro establishment (91.7% sprouting percentage and shoot length of 1.75 cm).”
e1“guava (P. guajava L.)”
Claim · model-written summary
The same establishment medium gave shoot length of 1.75 cm.
- Taxon
- guavaPsidium guajavae1
- Treatment
- Murashige and Skoog (MS) medium supplemented with 6-benzyl adenine (BA) at 8.9 M, plus indole-3-butyric acid (IBA) at 0.98 Me0
- Outcome
- shoot lengthe0
- Direction
- beste0
- Value
- 1.75 cme0
- Qualifier
- Autumne0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Explants collected in Autumn and cultured on Murashige and Skoog (MS) medium supplemented with 6-benzyl adenine (BA) at 8.9 M, plus indole-3-butyric acid (IBA) at 0.98 M showed the best response in the in vitro establishment (91.7% sprouting percentage and shoot length of 1.75 cm).”
e1“guava (P. guajava L.)”
Claim · model-written summary
BA was more effective than kinetin for average shoot number and length per explant.
- Taxon
- guavaPsidium guajavae1
- Treatment
- BAe0
- Outcome
- average number and length of shoots per explante0
- Direction
- more effectivee0
- Comparator
- kinetin (kin)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Regarding average number and length of shoots per explant, BA was more effective than kinetin (kin).”
e1“guava (P. guajava L.)”
Claim · model-written summary
Solid half-strength MS medium with IBA yielded maximum rooting of 66.7%.
- Taxon
- guavaPsidium guajavae1
- Treatment
- solid half-strength MS medium containing indol-3-butyric acid (IBA) at 9.8 Me0
- Outcome
- rooting percentagee0
- Direction
- Maximume0
- Value
- 66.7%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maximum rooting percentage (66.7%) occurred on solid half-strength MS medium containing indol-3-butyric acid (IBA) at 9.8 M.”
e1“guava (P. guajava L.)”
Claim · model-written summary
Half of rooted plantlets acclimatized in a greenhouse and subsequently established outside it.
- Taxon
- guavaPsidium guajavae1
- Outcome
- rooted plantlets were successfully acclimatizede0
- Direction
- successfullye0
- Value
- 50%e0
- Setting
- greenhousee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fifty percent (50%) rooted plantlets were successfully acclimatized in the greenhouse in soil mixture of sand, grand soil and peat moss at equal volumes, and subsequently established outside the greenhouse.”
e1“guava (P. guajava L.)”
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in vitro · growth · Psidium guajava (guava)Positive and negative results
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See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.