Abstract
Present investigation on sweet cherry (Prunus avium L.) cv. Bigarreau Noir Grossa was carried out on its micropropagation. Surface sterilisation of shoot tip explant with 0.1% HgCl2for 10 min. yielded maximum aseptic cultures and explant survival. Using explants from forced stock stem plant cuttings significantly improved culture initiation. Maximum explant establishment (50.57%) was observed on Murashige and Skoog (1962) medium supplemented with BAP + kinetin (0.25 + 0.25 mg l−1). Treatment BAP + kinetin (0.25 + 0.25 mg l−1) accounted for maximum proliferating cultures (99.96%) with highest multiplication efficiency in terms of proliferation grade (4.0) and shoots/explant (17.28). Rooting was carried out by incubation of micro-shoots in IBA supplemented MS medium for 10 days under darkness followed by their transfer on to hormone-free MS medium with incubation under light conditions. Micro-shoots equal to or greater than 10 mm in length gave good rooting. Auxin IBA (2.50 mg l−1) was found most effective, which not only gave the highest rooting (86.33%) but also maximised root number/shoot (4.90) and root length (44.33 mm).
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Claims 10
Claim · model-written summary
Sterilising shoot tip explants with 0.1% HgCl2 for 10 min gave maximum aseptic cultures and explant survival.
- Taxon
- sweet cherry
Prunus aviumt0 - Treatment
- Surface sterilisation of shoot tip explant with 0.1% HgCl2for 10 mine0
- Outcome
- aseptic cultures and explant survivale0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Surface sterilisation of shoot tip explant with 0.1% HgCl2for 10 min. yielded maximum aseptic cultures and explant survival”
t0“sweet cherry”
Claim · model-written summary
Explants from forced stock stem plant cuttings significantly improved culture initiation.
- Taxon
- sweet cherryPrunus aviumt0
- Treatment
- explants from forced stock stem plant cuttingse0
- Outcome
- culture initiatione0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using explants from forced stock stem plant cuttings significantly improved culture initiation”
t0“sweet cherry”
Claim · model-written summary
Maximum explant establishment (50.57%) occurred on MS medium with BAP + kinetin (0.25 + 0.25 mg l-1).
- Taxon
- sweet cherryPrunus aviumt0
- Treatment
- Murashige and Skoog (1962) medium supplemented with BAP + kinetin (0.25 + 0.25 mg l−1)e0
- Outcome
- explant establishmente0
- Direction
- Maximume0
- Value
- 50.57%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maximum explant establishment (50.57%) was observed on Murashige and Skoog (1962) medium supplemented with BAP + kinetin (0.25 + 0.25 mg l−1)”
t0“sweet cherry”
Claim · model-written summary
BAP + kinetin (0.25 + 0.25 mg l-1) gave maximum proliferating cultures (99.96%).
- Taxon
- sweet cherryPrunus aviumt0
- Treatment
- BAP + kinetin (0.25 + 0.25 mg l−1)e0
- Outcome
- proliferating culturese0
- Direction
- maximume0
- Value
- 99.96%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment BAP + kinetin (0.25 + 0.25 mg l−1) accounted for maximum proliferating cultures (99.96%) with highest multiplication efficiency in terms of proliferation grade (4.0) and shoots/explant (17.28)”
t0“sweet cherry”
Claim · model-written summary
BAP + kinetin (0.25 + 0.25 mg l-1) gave the highest proliferation grade (4.0).
- Taxon
- sweet cherryPrunus aviumt0
- Treatment
- BAP + kinetin (0.25 + 0.25 mg l−1)e0
- Outcome
- proliferation gradee0
- Direction
- higheste0
- Value
- 4.0e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment BAP + kinetin (0.25 + 0.25 mg l−1) accounted for maximum proliferating cultures (99.96%) with highest multiplication efficiency in terms of proliferation grade (4.0) and shoots/explant (17.28)”
t0“sweet cherry”
Claim · model-written summary
BAP + kinetin (0.25 + 0.25 mg l-1) gave the highest number of shoots per explant (17.28).
- Taxon
- sweet cherryPrunus aviumt0
- Treatment
- BAP + kinetin (0.25 + 0.25 mg l−1)e0
- Outcome
- shoots/explante0
- Direction
- higheste0
- Value
- 17.28e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment BAP + kinetin (0.25 + 0.25 mg l−1) accounted for maximum proliferating cultures (99.96%) with highest multiplication efficiency in terms of proliferation grade (4.0) and shoots/explant (17.28)”
t0“sweet cherry”
Claim · model-written summary
Micro-shoots of 10 mm or longer gave good rooting.
- Taxon
- sweet cherryPrunus aviumt0
- Outcome
- good rootinge0
- Qualifier
- Micro-shoots equal to or greater than 10 mm in lengthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Micro-shoots equal to or greater than 10 mm in length gave good rooting”
t0“sweet cherry”
Claim · model-written summary
Auxin IBA (2.50 mg l-1) was most effective and gave the highest rooting (86.33%).
- Taxon
- sweet cherryPrunus aviumt0
- Treatment
- Auxin IBA (2.50 mg l−1)e0
- Outcome
- rootinge0
- Direction
- higheste0
- Value
- 86.33%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Auxin IBA (2.50 mg l−1) was found most effective, which not only gave the highest rooting (86.33%) but also maximised root number/shoot (4.90) and root length (44.33 mm)”
t0“sweet cherry”
Claim · model-written summary
Auxin IBA (2.50 mg l-1) maximised root number per shoot (4.90).
- Taxon
- sweet cherryPrunus aviumt0
- Treatment
- Auxin IBA (2.50 mg l−1)e0
- Outcome
- root number/shoote0
- Direction
- maximisede0
- Value
- 4.90e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Auxin IBA (2.50 mg l−1) was found most effective, which not only gave the highest rooting (86.33%) but also maximised root number/shoot (4.90) and root length (44.33 mm)”
t0“sweet cherry”
Claim · model-written summary
Auxin IBA (2.50 mg l-1) maximised root length (44.33 mm).
- Taxon
- sweet cherryPrunus aviumt0
- Treatment
- Auxin IBA (2.50 mg l−1)e0
- Outcome
- root lengthe0
- Direction
- maximisede0
- Value
- 44.33 mme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Auxin IBA (2.50 mg l−1) was found most effective, which not only gave the highest rooting (86.33%) but also maximised root number/shoot (4.90) and root length (44.33 mm)”
t0“sweet cherry”
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