Abstract
We investigated the transcript accumulation patterns of all three subunits of heterotrimeric G-proteins (Gα1 and 2, Gβ, and Gγ) in pea under stimulation of two soil-inhabiting rhizosphere microbes Pseudomonas fluorescens OKC and Trichoderma asperellum T42. The microbes were either applied individually or co-inoculated and the transcript accumulation patterns were also investigated after challenging the same plants with a fungal biotrophic pathogen Erysiphe pisi. We observed that mostly the transcripts of Gα 1 and 2 subunits were accumulated when the plants were treated with the microbes (OKC and T42) either individually or co-inoculated. However, transcript accumulations of Gα subunits were highest in the T42 treatment particularly under the challenge of the biotroph. Transcript accumulations of the other two subunits Gβ and Gγ were either basal or even lower than the basal level. There was an indication for involvement of JA-mediated pathway in the same situations as activation of LOX1 and COI1 were relatively enhanced in the microbe co-inoculated treatments. Non-increment of SA content as well as transcripts of SA-dependent PR1 suggested non-activation of the SA-mediated signal transduction in the interaction of pea with E. pisi under the stimuli of OKC and T42. Gα1 and 2 transcript accumulations were further correlated with peroxidases activities, H2O2 generation and accumulation in ABA in pea leaves under OKC and T42 stimulations and all these activities were positively correlated with stomata closure at early stage of the biotroph challenge. The microbe-induced physiological responses in pea leaves finally led to reduced E. pisi development particularly in OKC and T42 co-inoculated plants. We conclude that OKC and T42 pretreatment stimulate transcript accumulations of the Gα1 and Gα2 subunits of the heterotrimeric G protein, peroxidases activities and phenol accumulation in pea during infection by E. pisi. The signal transduction was possibly mediated through JA in pea under the stimulus of the microbes and the cumulative effect of the co-inoculated microbes had a suppressive effect on E. pisi conidial development on pea leaves.
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Claims 11
Claim · model-written summary
Microbe treatments accumulated pea Gα1 and Gα2 transcripts.
- Taxon
- Pea
Lathyrus oleraceust0 - Treatment
- microbes (OKC and T42)e0
- Gene
- Gα 1 and 2e0
- Outcome
- transcripts of Gα 1 and 2 subunitse0
- Direction
- accumulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mostly the transcripts of Gα 1 and 2 subunits were accumulated when the plants were treated with the microbes (OKC and T42) either individually or co-inoculated”
t0“Pea”
Claim · model-written summary
T42 produced the highest Gα transcript accumulation under pathogen challenge.
- Taxon
- PeaLathyrus oleraceust0
- Treatment
- T42 treatmente0
- Gene
- Gαe0
- Outcome
- transcript accumulations of Gα subunitse0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcript accumulations of Gα subunits were highest in the T42 treatment particularly under the challenge of the biotroph”
t0“Pea”
Claim · model-written summary
Gβ and Gγ transcripts remained basal or below basal.
- Taxon
- PeaLathyrus oleraceust0
- Gene
- Gβ and Gγe0
- Outcome
- Transcript accumulationse0
- Direction
- basal or even lowere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcript accumulations of the other two subunits Gβ and Gγ were either basal or even lower than the basal level.”
t0“Pea”
Claim · model-written summary
Microbe co-inoculation relatively enhanced LOX1 and COI1 activation, suggesting JA pathway involvement.
- Taxon
- PeaLathyrus oleraceust0
- Treatment
- microbe co-inoculated treatmentse0
- Gene
- LOX1 and COI1e0
- Compound
- JAe0
- Outcome
- activation of LOX1 and COI1e0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There was an indication for involvement of JA-mediated pathway in the same situations as activation of LOX1 and COI1 were relatively enhanced in the microbe co-inoculated treatments.”
t0“Pea”
Claim · model-written summary
SA content did not increase under microbial stimulation and pathogen interaction.
- Taxon
- peaLathyrus oleraceuse0
- Interactor
- E. pisi
.jpg?width=60)
Erysiphe pisie0pathogen - Outcome
- SA contente0
- Direction
- Non-incremente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Non-increment of SA content as well as transcripts of SA-dependent PR1 suggested non-activation of the SA-mediated signal transduction in the interaction of pea with E. pisi under the stimuli of OKC and T42.”
Claim · model-written summary
PR1 transcripts did not increase under microbial stimulation and pathogen interaction.
- Taxon
- peaLathyrus oleraceuse0
- Interactor
- E. pisiErysiphe pisie0pathogen
- Gene
- PR1e0
- Outcome
- transcripts of SA-dependent PR1e0
- Direction
- Non-incremente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Non-increment of SA content as well as transcripts of SA-dependent PR1 suggested non-activation of the SA-mediated signal transduction in the interaction of pea with E. pisi under the stimuli of OKC and T42.”
Claim · model-written summary
Gα transcript accumulation correlated with peroxidase activity, H2O2 generation and ABA accumulation.
- Taxon
- peaLathyrus oleraceuse0
- Gene
- Gα1 and 2e0
- Outcome
- peroxidases activities, H2O2 generation and accumulation in ABAe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gα1 and 2 transcript accumulations were further correlated with peroxidases activities, H2O2 generation and accumulation in ABA in pea leaves under OKC and T42 stimulations”
Claim · model-written summary
Those activities correlated positively with early stomatal closure during pathogen challenge.
- Taxon
- PeaLathyrus oleraceust0
- Organ / stage
- stomatae0
- Outcome
- stomata closuree0
- Direction
- positively correlatede0
- Qualifier
- early stage of the biotroph challengee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all these activities were positively correlated with stomata closure at early stage of the biotroph challenge”
t0“Pea”
Claim · model-written summary
Microbe-induced responses reduced E. pisi development, particularly with co-inoculation.
- Taxon
- peaLathyrus oleraceuse0
- Treatment
- OKC and T42 co-inoculatede0
- Interactor
- E. pisiErysiphe pisie0pathogen
- Outcome
- E. pisi developmente0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The microbe-induced physiological responses in pea leaves finally led to reduced E. pisi development particularly in OKC and T42 co-inoculated plants.”
Claim · model-written summary
OKC and T42 pretreatment stimulated peroxidase activity and phenol accumulation during infection.
- Taxon
- peaLathyrus oleraceuse0
- Treatment
- OKC and T42 pretreatmente0
- Interactor
- E. pisiErysiphe pisie0pathogen
- Outcome
- peroxidases activities and phenol accumulatione0
- Direction
- stimulatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OKC and T42 pretreatment stimulate transcript accumulations of the Gα1 and Gα2 subunits of the heterotrimeric G protein, peroxidases activities and phenol accumulation in pea during infection by E. pisi.”
Claim · model-written summary
The co-inoculated microbes suppressed E. pisi conidial development.
- Taxon
- peaLathyrus oleraceuse0
- Treatment
- co-inoculated microbese0
- Interactor
- E. pisiErysiphe pisie0pathogen
- Outcome
- E. pisi conidial developmente0
- Direction
- suppressivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the cumulative effect of the co-inoculated microbes had a suppressive effect on E. pisi conidial development on pea leaves.”
The same questions in other papers
inoculation · Erysiphe pisi · Lathyrus oleraceus (pea)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
1 paper more in this group
See all 5 papers →inoculation · molecular: expression · Lathyrus oleraceus (pea)Positive and negative results
6 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.