Abstract
In the present investigation, the influence of different forms of cytokinins, auxins and polyamines were tested for mass multiplication and regeneration of cotton. Initially, for the identification of effective concentration for multiple shoot induction, various concentrations of BAP, Kin and 2iP along with IAA and NAA were tested. Among tested concentrations, media fortified with MS salts; B5 vitamins; 30 g/l, glucose; 2.0 mg/l, 2iP; 2.0 mg/l, IAA and 0.7 % agar showed best response for multiplication of shoot tip explants (20 shoots per shoot tip explants). In nodal explants, maximum of 18.6 shoots were obtained in the media fortified with MS salts, B5 vitamins, 30 g/l, glucose, 2.0 mg/l, 2iP, 1.0 mg/l, NAA and 0.7 % agar. Effect of different concentrations of polyamines like spermidine and putrescine were also tested along with the above said multiplication media. Among the various treatments, 20 mg/l of putrescine showed best response and the multiple of shoots were increased to 26.5 shoots per shoot tip explants and 24.5 shoots per nodal explants. Elongation of shoots was achieved on multiple shoot induction medium. Significant number of roots were initiated in the medium supplemented with MS salts, vitamin B5 and IBA (2.0 mg/l). The frequency of root induction was increased by addition of, PVP (10 mg/l) along with root induction medium and after 2 weeks, the roots reached the maximum length of 22 cm. Further, these plantlets were hardened by using sand, soil and vermiculate in 1:1:1 ratio. The hardened plants were transferred to the environmental growth chamber for proper acclimatization. The hardened plants were then transferred to field for boll yielding and they exhibited 100% survival.
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Claims 9
Claim · model-written summary
A medium containing 2iP and IAA gave the best shoot tip multiplication response, with 20 shoots per explant.
- Taxon
- cotton
Gossypium hirsutumt0 - Organ / stage
- shoot tip explantse0
- Treatment
- 2.0 mg/l, 2iP; 2.0 mg/l, IAAe0
- Outcome
- multiplication of shoot tip explantse0
- Direction
- best responsee0
- Value
- 20 shoots per shoot tip explantse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton (Gossypium hirsutum L. cv. SVPR2)”
e0“media fortified with MS salts; B5 vitamins; 30 g/l, glucose; 2.0 mg/l, 2iP; 2.0 mg/l, IAA and 0.7 % agar showed best response for multiplication of shoot tip explants (20 shoots per shoot tip explants)”
Claim · model-written summary
The NAA and 2iP medium yielded a maximum of 18.6 shoots from nodal explants.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- nodal explantse0
- Treatment
- 2.0 mg/l, 2iP, 1.0 mg/l, NAAe0
- Outcome
- shootse0
- Direction
- maximume0
- Value
- 18.6 shootse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton (Gossypium hirsutum L. cv. SVPR2)”
e0“In nodal explants, maximum of 18.6 shoots were obtained in the media fortified with MS salts, B5 vitamins, 30 g/l, glucose, 2.0 mg/l, 2iP, 1.0 mg/l, NAA and 0.7 % agar.”
Claim · model-written summary
Putrescine at 20 mg/l increased shoot multiplication to 26.5 shoots per shoot tip explant.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- shoot tip explantse0
- Treatment
- 20 mg/l of putrescinee0
- Outcome
- multiple of shootse0
- Direction
- increasede0
- Value
- 26.5 shoots per shoot tip explantse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton (Gossypium hirsutum L. cv. SVPR2)”
e0“20 mg/l of putrescine showed best response and the multiple of shoots were increased to 26.5 shoots per shoot tip explants and 24.5 shoots per nodal explants”
Claim · model-written summary
Putrescine at 20 mg/l increased shoot multiplication to 24.5 shoots per nodal explant.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- nodal explantse0
- Treatment
- 20 mg/l of putrescinee0
- Outcome
- multiple of shootse0
- Direction
- increasede0
- Value
- 24.5 shoots per nodal explantse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton (Gossypium hirsutum L. cv. SVPR2)”
e0“20 mg/l of putrescine showed best response and the multiple of shoots were increased to 26.5 shoots per shoot tip explants and 24.5 shoots per nodal explants”
Claim · model-written summary
Shoots elongated on the multiple shoot induction medium.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- multiple shoot induction mediume0
- Outcome
- Elongation of shootse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton (Gossypium hirsutum L. cv. SVPR2)”
e0“Elongation of shoots was achieved on multiple shoot induction medium.”
Claim · model-written summary
A medium containing IBA initiated a significant number of roots.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- rootse0
- Treatment
- medium supplemented with MS salts, vitamin B5 and IBA (2.0 mg/l)e0
- Outcome
- rootse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton (Gossypium hirsutum L. cv. SVPR2)”
e0“Significant number of roots were initiated in the medium supplemented with MS salts, vitamin B5 and IBA (2.0 mg/l).”
Claim · model-written summary
Adding PVP to root induction medium increased root induction frequency.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- addition of, PVP (10 mg/l)e0
- Outcome
- frequency of root inductione0
- Direction
- increasede0
- Comparator
- root induction mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton (Gossypium hirsutum L. cv. SVPR2)”
e0“The frequency of root induction was increased by addition of, PVP (10 mg/l) along with root induction medium”
Claim · model-written summary
Roots reached a maximum length of 22 cm after two weeks with PVP added to the root induction medium.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- PVP (10 mg/l)e0
- Outcome
- roots reached the maximum lengthe0
- Direction
- maximume0
- Value
- 22 cme0
- Qualifier
- after 2 weekse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton (Gossypium hirsutum L. cv. SVPR2)”
e0“PVP (10 mg/l) along with root induction medium and after 2 weeks, the roots reached the maximum length of 22 cm”
Claim · model-written summary
Hardened cotton plantlets transferred to the field exhibited 100% survival.
- Taxon
- cottonGossypium hirsutumt0
- Outcome
- survivale0
- Value
- 100%e0
- Setting
- fielde0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton (Gossypium hirsutum L. cv. SVPR2)”
e0“The hardened plants were then transferred to field for boll yielding and they exhibited 100% survival.”
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