Abstract
Abiotic stress responses are under the strict control of transcriptional regulation. Gene stacking approach using two to three transcription factors (TFs), which aid in stable transcription would be promising. An attempt was made to co express a basal transcription factor (AhBTF3); Nuclear factor (AhNFYA7), an activator protein and zinc finger protein (EcZF), a stress inducible TF. It is hypothesised that a combination of different classes of TFs enhances transcriptional stability that can regulate stress acclimation. The gene cassette harbouring NFYA7, BTF3 and ZF expressed under rbcS, Ubi and rd29A promoters respectively, was cloned in the destination vector pi12GW (PsAKR1 as selectable marker), by modified gateway cloning strategy. Agrobacterium mediated in planta transformation technique was used to generate rice (AC-39020) transgenic plants. The putative transgenics were screened for glyphosate tolerance and confirmed by PCR and RT-PCR. The select lines were subjected for physiological characterisation to assess their stress response. The transformants screened for glyphosate tolerance exhibited varied response (Fig. 1). The select lines maintained higher chlorophyll stability when subjected for excised leaf disc stress tolerance assay using PEG and NaCl (Fig. 2A and B). The transgenics exhibited better growth as evidenced by plant height and seed yield (Fig. 2C and D). The promising transgenic lines were challenged with different abiotic stresses using NaCl, PEG and cadmium chloride which revealed their superior performance at seedling level (Fig. 3A). The integration of marker gene and expression of each transgene was confirmed by PCR (Fig. 3B) and RT-PCR respectively (Fig. 3C). Co expression of stress relevant TFs can enhance the levels of tolerance tomultiple abiotic stresses as demonstrated in this study. The roles of individual TF in modulating cellular stress responses need to be examined.
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Claims 6
Claim · model-written summary
Select transgenic lines maintained higher chlorophyll stability in an excised leaf disc assay with PEG and NaCl.
- Taxon
- Oryza sativa L
Oryza sativat0 - Material
- select linese0
- Organ / stage
- excised leaf disce0
- Treatment
- excised leaf disc stress tolerance assay using PEG and NaCle0
- Outcome
- chlorophyll stabilitye0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice (Oryza sativa L)”
e0“The select lines maintained higher chlorophyll stability when subjected for excised leaf disc stress tolerance assay using PEG and NaCl”
Claim · model-written summary
Transformants screened for glyphosate tolerance showed varied responses.
- Taxon
- Oryza sativa LOryza sativat0
- Material
- transformantse1
- Outcome
- glyphosate tolerancee1
- Qualifier
- varied responsee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice (Oryza sativa L)”
e1“The transformants screened for glyphosate tolerance exhibited varied response”
Claim · model-written summary
Transgenic rice exhibited better growth, with higher plant height and seed yield.
- Taxon
- Oryza sativa LOryza sativat0
- Material
- transgenicse2
- Outcome
- plant height and seed yielde2
- Direction
- bettere2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice (Oryza sativa L)”
e2“The transgenics exhibited better growth as evidenced by plant height and seed yield”
Claim · model-written summary
Promising transgenic lines showed superior performance at seedling level under NaCl, PEG and cadmium chloride stress.
- Taxon
- Oryza sativa LOryza sativat0
- Material
- transgenic linese3
- Organ / stage
- seedlinge3
- Treatment
- NaCl, PEG and cadmium chloridee3
- Outcome
- performancee3
- Direction
- superiore3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice (Oryza sativa L)”
e3“The promising transgenic lines were challenged with different abiotic stresses using NaCl, PEG and cadmium chloride which revealed their superior performance at seedling level”
Claim · model-written summary
Integration of the marker gene and expression of each transgene were confirmed by PCR and RT-PCR.
- Taxon
- Oryza sativa LOryza sativat0
- Outcome
- integration of marker gene and expression of each transgenee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice (Oryza sativa L)”
e4“The integration of marker gene and expression of each transgene was confirmed by PCR (Fig. 3B) and RT-PCR respectively”
Claim · model-written summary
Co-expression of stress-relevant transcription factors enhanced levels of tolerance to multiple abiotic stresses.
- Taxon
- Oryza sativa LOryza sativat0
- Treatment
- Co expression of stress relevant TFse5
- Outcome
- levels of tolerancee5
- Direction
- enhancee5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice (Oryza sativa L)”
e5“Co expression of stress relevant TFs can enhance the levels of tolerance”
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