Abstract
Cytokinins in plants are crucial for numerous biological processes, including seed germination, cell division and differentiation, floral initiation and adaptation to abiotic stresses. The salt stress can promote reactive oxygen species (ROS) production in plants which are highly toxic and ultimately results in oxidative stress. However, the correlation between endogenous cytokinin production and ROS homeostasis in responding to salt stress is poorly understood. In this study, we analyzed the correlation of overexpressing the cytokinin biosynthetic gene AtIPT8 (adenosine phosphate-isopentenyl transferase 8) and the response of salt stress in Arabidopsis. Overproduction of cytokinins, which was resulted by the inducible overexpression of AtIPT8, significantly inhibited the primary root growth and true leaf emergence, especially under the conditions of exogenous salt, glucose and mannitol treatments. Upon cytokinin overproduction, the salt stress resistance was declined, and resulted in less survival rates and chlorophyll content. Interestingly, ROS production was obviously increased with the salt treatment, accompanied by endogenously overproduced cytokinins. The activities of catalase (CAT) and superoxide dismutase (SOD), which are responsible for scavenging ROS, were also affected. Transcription profiling revealed that the differential expressions of ROS-producing and scavenging related genes, the photosynthesis-related genes and stress responsive genes were existed in transgenic plants of overproducing cytokinins. Our results suggested that broken in the homeostasis of cytokinins in plant cells could modulate the salt stress responses through a ROS-mediated regulation in Arabidopsis.
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Claims 7
Claim · model-written summary
Cytokinin overproduction from inducible AtIPT8 expression significantly inhibited primary root growth and true leaf emergence, especially under exogenous salt, glucose and mannitol.
- Taxon
- Arabidopsis Thaliana
Arabidopsis thalianat0 - Treatment
- inducible overexpression of AtIPT8e1
- Outcome
- primary root growth and true leaf emergencee1
- Direction
- significantly inhibitede1
- Qualifier
- especially under the conditions of exogenous salt, glucose and mannitol treatmentse2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis Thaliana”
e1“Overproduction of cytokinins, which was resulted by the inducible overexpression of AtIPT8, significantly inhibited the primary root growth and true leaf emergence”
e2“especially under the conditions of exogenous salt, glucose and mannitol treatments”
Claim · model-written summary
Upon cytokinin overproduction, salt stress resistance declined.
- Taxon
- Arabidopsis ThalianaArabidopsis thalianat0
- Treatment
- cytokinin overproductione3
- Outcome
- salt stress resistancee3
- Direction
- declinede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis Thaliana”
e3“Upon cytokinin overproduction, the salt stress resistance was declined, and resulted in less survival rates and chlorophyll content”
Claim · model-written summary
Upon cytokinin overproduction, survival rates and chlorophyll content were lower under salt stress.
- Taxon
- Arabidopsis ThalianaArabidopsis thalianat0
- Treatment
- cytokinin overproductione3
- Outcome
- survival rates and chlorophyll contente3
- Direction
- lesse3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis Thaliana”
e3“Upon cytokinin overproduction, the salt stress resistance was declined, and resulted in less survival rates and chlorophyll content”
Claim · model-written summary
ROS production was obviously increased by salt treatment in plants with overproduced cytokinins.
- Taxon
- Arabidopsis ThalianaArabidopsis thalianat0
- Treatment
- salt treatmente4
- Outcome
- ROS productione4
- Direction
- obviously increasede4
- Qualifier
- accompanied by endogenously overproduced cytokininse4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis Thaliana”
e4“ROS production was obviously increased with the salt treatment, accompanied by endogenously overproduced cytokinins”
Claim · model-written summary
Activities of catalase and superoxide dismutase were also affected in cytokinin-overproducing plants.
- Taxon
- Arabidopsis ThalianaArabidopsis thalianat0
- Outcome
- activities of catalase (CAT) and superoxide dismutase (SOD)e5
- Qualifier
- were also affectede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis Thaliana”
e5“The activities of catalase (CAT) and superoxide dismutase (SOD), which are responsible for scavenging ROS, were also affected”
Claim · model-written summary
Transcription profiling showed differential expression of ROS-related, photosynthesis-related and stress-responsive genes in cytokinin-overproducing plants.
- Taxon
- Arabidopsis ThalianaArabidopsis thalianat0
- Outcome
- differential expressions of ROS-producing and scavenging related genese6
- Qualifier
- photosynthesis-related genes and stress responsive genese6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis Thaliana”
e6“Transcription profiling revealed that the differential expressions of ROS-producing and scavenging related genes, the photosynthesis-related genes and stress responsive genes were existed in transgenic plants of overproducing cytokinins”
Claim · model-written summary
Broken cytokinin homeostasis may modulate salt stress responses through ROS-mediated regulation in Arabidopsis (authors suggest).
- Taxon
- ArabidopsisArabidopsise7
- Compound
- cytokininse7
- Outcome
- salt stress responsese7
- Qualifier
- through a ROS-mediated regulatione7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“our results suggested that broken in the homeostasis of cytokinins in plant cells could modulate the salt stress responses through a ROS-mediated regulation in Arabidopsis”
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