Abstract
Plant responses to abiotic stress include various modifications in amino acid metabolism. By using a hydroponic culture system, we systematically investigate modification in amino acid profiles and the proteome of Arabidopsis thaliana leaves during initial recovery from low water potential or high salinity. Both treatments elicited oxidative stress leading to a biphasic stress response during recovery. Degradation of highly abundant proteins such as subunits of photosystems and ribosomes contributed to an accumulation of free amino acids. Catabolic pathways for several low abundant amino acids were induced indicating their usage as an alternative respiratory substrate to compensate for the decreased photosynthesis. Our results demonstrate that rapid detoxification of potentially detrimental amino acids such as Lys is a priority during the initial stress recovery period. The content of Pro, which acts as a compatible osmolyte during stress, was adjusted by balancing its synthesis and catabolism both of which were induced both during and after stress treatments. The production of amino acid derived secondary metabolites was up-regulated specifically during the recovery period, and our dataset also indicates increased synthesis rates of the precursor amino acids. Overall, our results support a tight relationship between amino acid metabolism and stress responses.
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Claims 9
Claim · model-written summary
Low water potential elicited oxidative stress and a biphasic response during Arabidopsis recovery.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae1 - Treatment
- low water potentiale1
- Outcome
- oxidative stresse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both treatments elicited oxidative stress leading to a biphasic stress response during recovery.”
e1“Arabidopsis thaliana leaves during initial recovery from low water potential or high salinity”
Claim · model-written summary
High salinity elicited oxidative stress and a biphasic response during Arabidopsis recovery.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- high salinitye1
- Outcome
- oxidative stresse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both treatments elicited oxidative stress leading to a biphasic stress response during recovery.”
e1“Arabidopsis thaliana leaves during initial recovery from low water potential or high salinity”
Claim · model-written summary
Degradation of abundant photosystem and ribosome proteins contributed to free amino acid accumulation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Outcome
- accumulation of free amino acidse0
- Qualifier
- Degradation of highly abundant proteins such as subunits of photosystems and ribosomese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Degradation of highly abundant proteins such as subunits of photosystems and ribosomes contributed to an accumulation of free amino acids.”
e1“Arabidopsis thaliana leaves”
Claim · model-written summary
Catabolic pathways for several low-abundance amino acids were induced during recovery.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Outcome
- Catabolic pathways for several low abundant amino acids were inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Catabolic pathways for several low abundant amino acids were induced”
e1“Arabidopsis thaliana leaves”
Claim · model-written summary
The induced amino acid catabolic pathways indicate use as an alternative respiratory substrate compensating for reduced photosynthesis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae2
- Outcome
- alternative respiratory substratee0
- Qualifier
- decreased photosynthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“indicating their usage as an alternative respiratory substrate to compensate for the decreased photosynthesis”
e1“Catabolic pathways for several low abundant amino acids were induced”
e2“Arabidopsis thaliana leaves”
Claim · model-written summary
Rapid detoxification of potentially detrimental amino acids such as Lys was prioritized during initial stress recovery.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Outcome
- rapid detoxification of potentially detrimental amino acids such as Lyse0
- Qualifier
- initial stress recovery periode0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rapid detoxification of potentially detrimental amino acids such as Lys is a priority during the initial stress recovery period”
e1“Arabidopsis thaliana leaves”
Claim · model-written summary
Pro content was adjusted by induced synthesis and catabolism during and after stress.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Outcome
- content of Proe0
- Qualifier
- balancing its synthesis and catabolisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The content of Pro, which acts as a compatible osmolyte during stress, was adjusted by balancing its synthesis and catabolism both of which were induced both during and after stress treatments.”
e1“Arabidopsis thaliana leaves”
Claim · model-written summary
Production of amino-acid-derived secondary metabolites was upregulated during recovery.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Outcome
- production of amino acid derived secondary metabolitese0
- Direction
- up-regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The production of amino acid derived secondary metabolites was up-regulated specifically during the recovery period”
e1“Arabidopsis thaliana leaves”
Claim · model-written summary
The dataset indicates increased synthesis of precursor amino acids during recovery.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Outcome
- synthesis rates of the precursor amino acidse0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“our dataset also indicates increased synthesis rates of the precursor amino acids”
e1“Arabidopsis thaliana leaves”
The same questions in other papers
salinity · physiology: oxidative · Arabidopsis thaliana (thale cress)Results agree
9 papers in the same direction as this paper
- negative
- negative
- negative
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.