Abstract
The induction of the dehydration-responsive Arabidopsis gene, rd29B, is mediated mainly by abscisic acid (ABA). Promoter analysis of rd29B indicated that two ABA-responsive elements (ABREs) are required for the dehydration-responsive expression of rd29B as cis-acting elements. Three cDNAs encoding basic leucine zipper (bZIP)-type ABRE-binding proteins were isolated by using the yeast one-hybrid system and were designated AREB1, AREB2, and AREB3 (ABA-responsive element binding protein). Transcription of the AREB1 and AREB2 genes is up-regulated by drought, NaCl, and ABA treatment in vegetative tissues. In a transient transactivation experiment using Arabidopsis leaf protoplasts, both the AREB1 and AREB2 proteins activated transcription of a reporter gene driven by ABRE. AREB1 and AREB2 required ABA for their activation, because their transactivation activities were repressed in aba2 and abi1 mutants and enhanced in an era1 mutant. Activation of AREBs by ABA was suppressed by protein kinase inhibitors. These results suggest that both AREB1 and AREB2 function as transcriptional activators in the ABA-inducible expression of rd29B, and further that ABA-dependent posttranscriptional activation of AREB1 and AREB2, probably by phosphorylation, is necessary for their maximum activation by ABA. Using cultured Arabidopsis cells, we demonstrated that a specific ABA-activated protein kinase of 42-kDa phosphorylated conserved N-terminal regions in the AREB proteins.
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Claims 11
Claim · model-written summary
ABA mainly mediated induction of Arabidopsis rd29B.
- Taxon
- Arabidopsis
Arabidopsise0 - Gene
- rd29Be0
- Compound
- abscisic acide0
- Outcome
- inductione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The induction of the dehydration-responsive Arabidopsis gene, rd29B, is mediated mainly by abscisic acid (ABA).”
Claim · model-written summary
Two ABREs were required for dehydration-responsive rd29B expression.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- rd29Be0
- Outcome
- dehydration-responsive expression of rd29Be0
- Qualifier
- two ABA-responsive elements (ABREs)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two ABA-responsive elements (ABREs) are required for the dehydration-responsive expression of rd29B”
t0“Arabidopsis”
Claim · model-written summary
Three cDNAs encoding ABRE-binding proteins were isolated and designated AREB1, AREB2 and AREB3.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- AREB1, AREB2, and AREB3e0
- Outcome
- cDNAs encoding basic leucine zipper (bZIP)-type ABRE-binding proteins were isolatede0
- Value
- Three cDNAse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three cDNAs encoding basic leucine zipper (bZIP)-type ABRE-binding proteins were isolated by using the yeast one-hybrid system and were designated AREB1, AREB2, and AREB3”
t0“Arabidopsis”
Claim · model-written summary
Drought, NaCl and ABA up-regulated AREB1 and AREB2 transcription in vegetative tissues.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- vegetative tissuese0
- Treatment
- drought, NaCl, and ABA treatmente0
- Gene
- AREB1 and AREB2e0
- Outcome
- Transcriptione0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcription of the AREB1 and AREB2 genes is up-regulated by drought, NaCl, and ABA treatment in vegetative tissues.”
t0“Arabidopsis”
Claim · model-written summary
AREB1 and AREB2 activated transcription of an ABRE-driven reporter in Arabidopsis leaf protoplasts.
- Taxon
- ArabidopsisArabidopsise1
- Organ / stage
- leaf protoplastse1
- Treatment
- AREB1 and AREB2 proteinse0
- Gene
- AREB1 and AREB2e0
- Outcome
- transcription of a reporter gene driven by ABREe0
- Direction
- activatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both the AREB1 and AREB2 proteins activated transcription of a reporter gene driven by ABRE”
e1“Arabidopsis leaf protoplasts”
Claim · model-written summary
AREB transactivation was repressed in aba2 and abi1 mutants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- aba2 and abi1 mutantse0
- Outcome
- transactivation activitiese0
- Direction
- repressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“their transactivation activities were repressed in aba2 and abi1 mutants”
t0“Arabidopsis”
Claim · model-written summary
AREB transactivation was enhanced in an era1 mutant.
- Taxon
- ArabidopsisArabidopsist0
- Material
- era1 mutante0
- Outcome
- transactivation activitiese1
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enhanced in an era1 mutant”
e1“their transactivation activities were repressed in aba2 and abi1 mutants and enhanced in an era1 mutant”
t0“Arabidopsis”
Claim · model-written summary
Protein kinase inhibitors suppressed ABA activation of AREBs.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- protein kinase inhibitorse0
- Gene
- AREBse0
- Outcome
- Activation of AREBs by ABAe0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Activation of AREBs by ABA was suppressed by protein kinase inhibitors.”
t0“Arabidopsis”
Claim · model-written summary
The authors inferred AREB1 and AREB2 act as transcriptional activators for ABA-inducible rd29B expression.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- AREB1 and AREB2e0
- Outcome
- ABA-inducible expression of rd29Be0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both AREB1 and AREB2 function as transcriptional activators in the ABA-inducible expression of rd29B”
t0“Arabidopsis”
Claim · model-written summary
The authors proposed ABA-dependent posttranscriptional activation of AREB1 and AREB2, probably by phosphorylation, is needed for maximal activation.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- AREB1 and AREB2e0
- Outcome
- maximum activation by ABAe0
- Qualifier
- probably by phosphorylatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA-dependent posttranscriptional activation of AREB1 and AREB2, probably by phosphorylation, is necessary for their maximum activation by ABA”
t0“Arabidopsis”
Claim · model-written summary
An ABA-activated 42-kDa protein kinase phosphorylated conserved AREB N-terminal regions in cultured Arabidopsis cells.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- cultured Arabidopsis cellse0
- Gene
- ABA-activated protein kinasee0
- Outcome
- phosphorylated conserved N-terminal regions in the AREB proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using cultured Arabidopsis cells, we demonstrated that a specific ABA-activated protein kinase of 42-kDa phosphorylated conserved N-terminal regions in the AREB proteins.”
The same questions in other papers
(+)-abscisic acid · molecular: expression · ArabidopsisPositive and negative results
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- no effect
- positive
- positive
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See all 82 papers →drought · molecular: expression · ArabidopsisPositive and negative results
38 papers in the same direction as this paper · 8 in the opposite direction
- positive
- positive
- positive
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See all 47 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.