Abstract
Xanthomonas oryzae pv. oryzae causes bacterial blight, a serious disease of rice. Our analysis revealed that the X. oryzae pv. oryzae genome encodes genes responsible for iron uptake through FeoB (homolog of the major bacterial ferrous iron transporter) and a siderophore. A mutation in the X. oryzae pv. oryzae feoB gene causes severe virulence deficiency, growth deficiency in iron-limiting medium, and constitutive production of a siderophore. We identified an iron regulated xss gene cluster, in which xssABCDE (Xanthomonas siderophore synthesis) and xsuA (Xanthomonas siderophore utilization) genes encode proteins involved in biosynthesis and utilization of X. oryzae pv. oryzae siderophore. Mutations in the xssA, xssB, and xssE genes cause siderophore deficiency and growth restriction under iron-limiting conditions but are virulence proficient. An xsuA mutant displayed impairment in utilization of native siderophore, suggesting that XsuA acts as a specific receptor for a ferric-siderophore complex. Histochemical and fluorimetric assays with gusA fusions indicate that, during in planta growth, the feoB gene is expressed and that the xss operon is not expressed. This study represents the first report describing a role for feoB in virulence of any plant-pathogenic bacterium and the first functional characterization of a siderophore-biosynthetic gene cluster in any xanthomonad.
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Claims 10
Claim · model-written summary
A feoB mutation in Xanthomonas oryzae pv. oryzae causes severe virulence deficiency on rice.
- Taxon
- rice
Oryza sativae0 - Treatment
- A mutation in the X. oryzae pv. oryzae feoB genee1
- Interactor
- Xanthomonas oryzae pv. oryzae
Xanthomonas oryzae pv. oryzaee0pathogen - Gene
- feoBe1
- Outcome
- virulencee1
- Direction
- deficiencye1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Xanthomonas oryzae pv. oryzae causes bacterial blight, a serious disease of rice”
e1“A mutation in the X. oryzae pv. oryzae feoB gene causes severe virulence deficiency”
Claim · model-written summary
A feoB mutation causes growth deficiency of Xanthomonas oryzae pv. oryzae in iron-limiting medium.
- Taxon
- riceOryza sativae0
- Treatment
- A mutation in the X. oryzae pv. oryzae feoB genee1
- Interactor
- Xanthomonas oryzae pv. oryzaeXanthomonas oryzae pv. oryzaee0pathogen
- Gene
- feoBe1
- Outcome
- growth deficiency in iron-limiting mediume2
- Direction
- deficiencye2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Xanthomonas oryzae pv. oryzae causes bacterial blight, a serious disease of rice”
e1“A mutation in the X. oryzae pv. oryzae feoB gene causes severe virulence deficiency”
e2“growth deficiency in iron-limiting medium”
Claim · model-written summary
A feoB mutation makes Xanthomonas oryzae pv. oryzae produce siderophore constitutively.
- Taxon
- riceOryza sativae0
- Treatment
- A mutation in the X. oryzae pv. oryzae feoB genee1
- Interactor
- Xanthomonas oryzae pv. oryzaeXanthomonas oryzae pv. oryzaee0pathogen
- Gene
- feoBe1
- Outcome
- production of a siderophoree3
- Direction
- constitutivee3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Xanthomonas oryzae pv. oryzae causes bacterial blight, a serious disease of rice”
e1“A mutation in the X. oryzae pv. oryzae feoB gene causes severe virulence deficiency”
e3“constitutive production of a siderophore”
Claim · model-written summary
Mutations in xssA, xssB and xssE cause siderophore deficiency and growth restriction under iron-limiting conditions.
- Taxon
- riceOryza sativae0
- Treatment
- Mutations in the xssA, xssB, and xssE genese4
- Interactor
- Xanthomonas oryzae pv. oryzaeXanthomonas oryzae pv. oryzaee0pathogen
- Gene
- xssA, xssB, and xssEe4
- Outcome
- siderophore deficiency and growth restriction under iron-limiting conditionse4
- Direction
- deficiencye4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Xanthomonas oryzae pv. oryzae causes bacterial blight, a serious disease of rice”
e4“Mutations in the xssA, xssB, and xssE genes cause siderophore deficiency and growth restriction under iron-limiting conditions”
Claim · model-written summary
Mutations in xssA, xssB and xssE leave Xanthomonas oryzae pv. oryzae virulence on rice unchanged.
- Taxon
- riceOryza sativae0
- Treatment
- Mutations in the xssA, xssB, and xssE genese4
- Interactor
- Xanthomonas oryzae pv. oryzaeXanthomonas oryzae pv. oryzaee0pathogen
- Gene
- xssA, xssB, and xssEe4
- Outcome
- virulencee5
- Direction
- proficiente5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Xanthomonas oryzae pv. oryzae causes bacterial blight, a serious disease of rice”
e4“Mutations in the xssA, xssB, and xssE genes cause siderophore deficiency and growth restriction under iron-limiting conditions”
e5“but are virulence proficient”
Claim · model-written summary
An xsuA mutant is impaired in utilising native siderophore.
- Taxon
- riceOryza sativae0
- Treatment
- An xsuA mutante6
- Interactor
- Xanthomonas oryzae pv. oryzaeXanthomonas oryzae pv. oryzaee0pathogen
- Gene
- xsuAe6
- Outcome
- utilization of native siderophoree6
- Direction
- impairmente6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Xanthomonas oryzae pv. oryzae causes bacterial blight, a serious disease of rice”
e6“An xsuA mutant displayed impairment in utilization of native siderophore”
Claim · model-written summary
XsuA probably acts as a specific receptor for a ferric-siderophore complex.
- Taxon
- riceOryza sativae0
- Interactor
- Xanthomonas oryzae pv. oryzaeXanthomonas oryzae pv. oryzaee0pathogen
- Gene
- XsuAe7
- Outcome
- a specific receptor for a ferric-siderophore complexe7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Xanthomonas oryzae pv. oryzae causes bacterial blight, a serious disease of rice”
e7“suggesting that XsuA acts as a specific receptor for a ferric-siderophore complex”
Claim · model-written summary
The feoB gene is expressed by Xanthomonas oryzae pv. oryzae during growth in planta.
- Taxon
- riceOryza sativae0
- Interactor
- Xanthomonas oryzae pv. oryzaeXanthomonas oryzae pv. oryzaee0pathogen
- Gene
- feoBe1
- Outcome
- expressede1
- Setting
- in planta growthe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Xanthomonas oryzae pv. oryzae causes bacterial blight, a serious disease of rice”
e1“during in planta growth, the feoB gene is expressed”
Claim · model-written summary
The xss operon is not expressed by Xanthomonas oryzae pv. oryzae during growth in planta.
- Taxon
- riceOryza sativae0
- Interactor
- Xanthomonas oryzae pv. oryzaeXanthomonas oryzae pv. oryzaee0pathogen
- Gene
- xss operone2
- Outcome
- not expressede2
- Setting
- in planta growthe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Xanthomonas oryzae pv. oryzae causes bacterial blight, a serious disease of rice”
e1“during in planta growth, the feoB gene is expressed”
e2“the xss operon is not expressed”
Claim · model-written summary
An iron-regulated xss gene cluster was identified in Xanthomonas oryzae pv. oryzae.
- Taxon
- riceOryza sativae0
- Interactor
- Xanthomonas oryzae pv. oryzaeXanthomonas oryzae pv. oryzaee0pathogen
- Gene
- xss gene clustere1
- Outcome
- iron regulated xss gene clustere1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Xanthomonas oryzae pv. oryzae causes bacterial blight, a serious disease of rice”
e1“We identified an iron regulated xss gene cluster”
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