Abstract
As a key virulence strategy to cause bacterial leaf blight, Xanthomonas oryzae pv. oryzae (Xoo) injects into the plant cell DNA-binding proteins called transcription activator-like effectors (TALEs) that bind to effector-binding elements (EBEs) in a sequence-specific manner, resulting in host gene induction. TALEs AvrXa7, PthXo3, TalC and Tal5, found in geographically distant Xoo strains, all target OsSWEET14, thus considered as a pivotal TALE target acting as major susceptibility factor during rice-Xoo interactions. Here, we report the generation of an allele library of the OsSWEET14 promoter through stable expression of TALE-nuclease (TALEN) constructs in rice. The susceptibility level of lines carrying mutations in AvrXa7, Tal5 or TalC EBEs was assessed. Plants edited in AvrXa7 or Tal5 EBEs were resistant to bacterial strains relying on the corresponding TALE. Surprisingly, although indels within TalC EBE prevented OsSWEET14 induction in response to BAI3 wild-type bacteria relying on TalC, loss of TalC responsiveness failed to confer resistance to this strain. The TalC EBE mutant line was, however, resistant to a strain expressing an artificial SWEET14-inducing TALE whose EBE was also edited in this line. This work offers the first set of alleles edited in TalC EBE and uncovers a distinct, broader range of activities for TalC compared to AvrXa7 or Tal5. We propose the existence of additional targets for TalC beyond SWEET14, suggesting that TALE-mediated plant susceptibility may result from induction of several, genetically redundant, host susceptibility genes by a single effector.
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Claims 9
Claim · model-written summary
Rice lines edited in the AvrXa7 effector-binding element resisted bacterial strains relying on AvrXa7.
- Taxon
- rice
Oryza sativat0 - Treatment
- edited in AvrXa7e0
- Interactor
- Xanthomonas oryzae pv. oryzae
Xanthomonas oryzae pv. oryzaet0pathogen - Gene
- AvrXa7e0
- Outcome
- resistant to bacterial strains relying on the corresponding TALEe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice resistance to Xanthomonas oryzae pv. oryzae”
e0“Plants edited in AvrXa7 or Tal5 EBEs were resistant to bacterial strains relying on the corresponding TALE.”
Claim · model-written summary
Rice lines edited in the Tal5 effector-binding element resisted bacterial strains relying on Tal5.
- Taxon
- riceOryza sativat0
- Treatment
- edited in AvrXa7 or Tal5 EBEse0
- Interactor
- Xanthomonas oryzae pv. oryzaeXanthomonas oryzae pv. oryzaet0pathogen
- Gene
- Tal5e0
- Outcome
- resistant to bacterial strains relying on the corresponding TALEe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice resistance to Xanthomonas oryzae pv. oryzae”
e0“Plants edited in AvrXa7 or Tal5 EBEs were resistant to bacterial strains relying on the corresponding TALE.”
Claim · model-written summary
Indels in the TalC binding element prevented OsSWEET14 induction by BAI3 wild-type bacteria.
- Taxon
- riceOryza sativat0
- Treatment
- indels within TalC EBEe0
- Interactor
- Xanthomonas oryzae pv. oryzaeXanthomonas oryzae pv. oryzaet0pathogen
- Gene
- OsSWEET14e0
- Outcome
- OsSWEET14 inductione0
- Direction
- preventede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice resistance to Xanthomonas oryzae pv. oryzae”
e0“indels within TalC EBE prevented OsSWEET14 induction in response to BAI3 wild-type bacteria relying on TalC”
Claim · model-written summary
Loss of TalC responsiveness did not confer resistance to the BAI3 strain.
- Taxon
- riceOryza sativat0
- Treatment
- loss of TalC responsivenesse0
- Interactor
- Xanthomonas oryzae pv. oryzaeXanthomonas oryzae pv. oryzaet0pathogen
- Outcome
- resistance to this straine0
- Direction
- failed to confere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice resistance to Xanthomonas oryzae pv. oryzae”
e0“loss of TalC responsiveness failed to confer resistance to this strain”
Claim · model-written summary
The TalC binding-element mutant resisted a strain expressing an artificial SWEET14-inducing effector whose binding element was edited.
- Taxon
- riceOryza sativat0
- Material
- TalC EBE mutant linee0
- Interactor
- Xanthomonas oryzae pv. oryzaeXanthomonas oryzae pv. oryzaet0pathogen
- Outcome
- resistant to a strain expressing an artificial SWEET14-inducing TALEe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice resistance to Xanthomonas oryzae pv. oryzae”
e0“The TalC EBE mutant line was, however, resistant to a strain expressing an artificial SWEET14-inducing TALE whose EBE was also edited in this line.”
Claim · model-written summary
The work provides alleles edited in the TalC binding element.
- Taxon
- riceOryza sativat0
- Outcome
- first set of alleles edited in TalC EBEe0
- Comparator
- firste0
Not reportedValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice resistance to Xanthomonas oryzae pv. oryzae”
e0“This work offers the first set of alleles edited in TalC EBE”
Claim · model-written summary
TalC showed a broader range of activities than AvrXa7 or Tal5.
- Taxon
- riceOryza sativat0
- Gene
- TalCe0
- Outcome
- broader range of activitiese0
- Direction
- broadere0
- Comparator
- AvrXa7 or Tal5e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice resistance to Xanthomonas oryzae pv. oryzae”
e0“uncovers a distinct, broader range of activities for TalC compared to AvrXa7 or Tal5”
Claim · model-written summary
The authors propose that TalC has additional targets beyond SWEET14.
- Taxon
- riceOryza sativat0
- Gene
- TalCe0
- Outcome
- additional targets for TalC beyond SWEET14e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice resistance to Xanthomonas oryzae pv. oryzae”
e0“We propose the existence of additional targets for TalC beyond SWEET14”
Claim · model-written summary
The authors suggest that one effector may induce several genetically redundant host susceptibility genes.
- Taxon
- riceOryza sativat0
- Outcome
- plant susceptibilitye0
- Qualifier
- induction of several, genetically redundant, host susceptibility genes by a single effectore0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice resistance to Xanthomonas oryzae pv. oryzae”
e0“TALE-mediated plant susceptibility may result from induction of several, genetically redundant, host susceptibility genes by a single effector”
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See all 29 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.