Abstract
Isolates of the rice blast fungus Magnaporthe grisea that carry the gene encoding Avirulence Conferring Enzyme1 (ACE1) are specifically recognized by rice (Oryza sativa) cultivars carrying the resistance gene Pi33. This recognition enables resistant plants to activate a defense response. ACE1 was isolated by map-based cloning and encodes a putative hybrid between a polyketide synthase and a nonribosomal peptide synthetase, enzymes involved in microbial secondary metabolism. ACE1 is expressed exclusively during fungal penetration of host leaves, the time point at which plant defense reactions are triggered. Ace1 appears to be localized in the cytoplasm of the appressorium. Mutation of the putative catalytic site of the beta-ketoacyl synthase domain of Ace1 abolishes recognition of the fungus by resistant rice. This suggests that Ace1 biosynthetic activity is required for avirulence. Our results are consistent with the hypothesis that the fungal signal recognized by resistant rice plants is the secondary metabolite whose synthesis depends on Ace1.
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Claims 9
Claim · model-written summary
ACE1 carrying Magnaporthe grisea isolates are recognized by rice cultivars carrying Pi33.
- Taxon
- rice
Oryza sativae0 - Interactor
- Magnaporthe grisea
Pyricularia griseae0pathogen - Gene
- Pi33e0
- Outcome
- specifically recognizede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Isolates of the rice blast fungus Magnaporthe grisea that carry the gene encoding Avirulence Conferring Enzyme1 (ACE1) are specifically recognized by rice (Oryza sativa) cultivars carrying the resistance gene Pi33.”
Claim · model-written summary
Recognition activates defense in resistant rice.
- Taxon
- riceOryza sativat0
- Outcome
- activate a defense responsee0
- Qualifier
- recognitione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This recognition enables resistant plants to activate a defense response.”
t0“Rice”
Claim · model-written summary
ACE1 was isolated by map based cloning.
- Taxon
- riceOryza sativat0
- Gene
- ACE1e0
- Outcome
- isolated by map-based cloninge0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ACE1 was isolated by map-based cloning”
t0“Rice”
Claim · model-written summary
ACE1 encodes a putative polyketide synthase and nonribosomal peptide synthetase hybrid.
- Taxon
- riceOryza sativat0
- Gene
- ACE1e0
- Outcome
- putative hybrid between a polyketide synthase and a nonribosomal peptide synthetasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ACE1 was isolated by map-based cloning and encodes a putative hybrid between a polyketide synthase and a nonribosomal peptide synthetase”
t0“Rice”
Claim · model-written summary
ACE1 expression occurs exclusively during fungal penetration of host leaves.
- Taxon
- riceOryza sativat0
- Organ / stage
- leavese0
- Gene
- ACE1e0
- Outcome
- expressed exclusively during fungal penetration of host leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ACE1 is expressed exclusively during fungal penetration of host leaves”
t0“Rice”
Claim · model-written summary
Ace1 appears localized in the appressorium cytoplasm.
- Taxon
- riceOryza sativat0
- Gene
- Ace1e0
- Outcome
- localized in the cytoplasm of the appressoriume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ace1 appears to be localized in the cytoplasm of the appressorium.”
t0“Rice”
Claim · model-written summary
Mutation of the Ace1 catalytic site abolishes resistant rice recognition.
- Taxon
- riceOryza sativae0
- Treatment
- Mutation of the putative catalytic site of the beta-ketoacyl synthase domain of Ace1e0
- Interactor
- funguse0pathogen
- Gene
- Ace1e0
- Outcome
- recognition of the fungus by resistant ricee0
- Direction
- abolishese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutation of the putative catalytic site of the beta-ketoacyl synthase domain of Ace1 abolishes recognition of the fungus by resistant rice.”
Claim · model-written summary
Ace1 biosynthetic activity appears required for avirulence.
- Taxon
- riceOryza sativat0
- Gene
- Ace1e0
- Outcome
- avirulencee0
- Qualifier
- biosynthetic activity is requirede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This suggests that Ace1 biosynthetic activity is required for avirulence.”
t0“Rice”
Claim · model-written summary
The authors hypothesize a secondary metabolite dependent on Ace1 is the signal recognized by resistant rice.
- Taxon
- riceOryza sativae0
- Gene
- Ace1e0
- Outcome
- fungal signal recognized by resistant rice plantse0
- Qualifier
- secondary metabolite whose synthesis depends on Ace1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Our results are consistent with the hypothesis that the fungal signal recognized by resistant rice plants is the secondary metabolite whose synthesis depends on Ace1.”
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