Abstract
Xanthomonas oryzae pv. oryzae is the causative agent of rice bacterial leaf blight. While the type III secretion system of X. oryzae pv. oryzae is essential for virulence, the biochemical activities and virulence mechanisms of non-transcription activator-like (non-TAL) effectors delivered by this system are largely unknown. Here, by screening for non-TAL effectors that contribute to X. oryzae pv. oryzae virulence, we revealed that Xanthomonas outer protein K (XopK) inhibits pathogen-associated molecular pattern-triggered immunity upstream of mitogen-activated protein kinase cascades. Specifically, XopK interacted with and directly ubiquitinated rice somatic embryogenic receptor kinase 2 (OsSERK2), resulting in its degradation. Accordingly, mutation of a putative ubiquitin-conjugation enzyme (E2) binding site abolished XopK-induced degradation of OsSERK2 and compromised XopK-dependent virulence. As crucial immune regulators associated with a multitude of immune receptors, SERKs have been shown to be perturbed by Pseudomonas effectors via different mechanisms. Our study revealed a distinct perturbation mechanism of SERK activity via ubiquitination achieved by Xanthomonas non-TAL effector.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
XopK inhibits pattern-triggered immunity upstream of MAP kinase cascades.
- Taxon
- rice
Oryza sativae1 - Interactor
- Xanthomonas oryzae pv. oryzae
Xanthomonas oryzae pv. oryzaee2pathogen - Gene
- Xanthomonas outer protein K (XopK)e0
- Outcome
- pathogen-associated molecular pattern-triggered immunitye0
- Direction
- inhibitse0
- Qualifier
- upstream of mitogen-activated protein kinase cascadese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Xanthomonas outer protein K (XopK) inhibits pathogen-associated molecular pattern-triggered immunity upstream of mitogen-activated protein kinase cascades”
e1“rice bacterial leaf blight”
e2“Xanthomonas oryzae pv. oryzae is the causative agent of rice bacterial leaf blight”
Claim · model-written summary
XopK interacted with rice OsSERK2.
- Taxon
- riceOryza sativae0
- Interactor
- Xanthomonas oryzae pv. oryzaeXanthomonas oryzae pv. oryzaee1pathogen
- Gene
- rice somatic embryogenic receptor kinase 2 (OsSERK2)e0
- Outcome
- XopK interacted withe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“XopK interacted with and directly ubiquitinated rice somatic embryogenic receptor kinase 2 (OsSERK2)”
e1“Xanthomonas oryzae pv. oryzae”
Claim · model-written summary
XopK directly ubiquitinated rice OsSERK2.
- Taxon
- riceOryza sativae0
- Interactor
- Xanthomonas oryzae pv. oryzaeXanthomonas oryzae pv. oryzaee1pathogen
- Gene
- rice somatic embryogenic receptor kinase 2 (OsSERK2)e0
- Outcome
- directly ubiquitinatede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“XopK interacted with and directly ubiquitinated rice somatic embryogenic receptor kinase 2 (OsSERK2)”
e1“Xanthomonas oryzae pv. oryzae”
Claim · model-written summary
XopK-mediated ubiquitination resulted in OsSERK2 degradation.
- Taxon
- riceOryza sativae0
- Interactor
- Xanthomonas oryzae pv. oryzaeXanthomonas oryzae pv. oryzaee1pathogen
- Gene
- rice somatic embryogenic receptor kinase 2 (OsSERK2)e0
- Outcome
- degradatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“XopK interacted with and directly ubiquitinated rice somatic embryogenic receptor kinase 2 (OsSERK2), resulting in its degradation”
e1“Xanthomonas oryzae pv. oryzae”
Claim · model-written summary
Mutation of a putative E2 binding site abolished XopK-induced OsSERK2 degradation.
- Taxon
- riceOryza sativae1
- Treatment
- mutation of a putative ubiquitin-conjugation enzyme (E2) binding sitee0
- Interactor
- Xanthomonas oryzae pv. oryzaeXanthomonas oryzae pv. oryzaee2pathogen
- Outcome
- XopK-induced degradation of OsSERK2e0
- Direction
- abolishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mutation of a putative ubiquitin-conjugation enzyme (E2) binding site abolished XopK-induced degradation of OsSERK2”
e1“rice somatic embryogenic receptor kinase 2 (OsSERK2)”
e2“Xanthomonas oryzae pv. oryzae”
Claim · model-written summary
Mutation of a putative E2 binding site compromised XopK-dependent virulence.
- Taxon
- riceOryza sativae1
- Treatment
- mutation of a putative ubiquitin-conjugation enzyme (E2) binding sitee0
- Interactor
- Xanthomonas oryzae pv. oryzaeXanthomonas oryzae pv. oryzaee2pathogen
- Outcome
- XopK-dependent virulencee0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mutation of a putative ubiquitin-conjugation enzyme (E2) binding site abolished XopK-induced degradation of OsSERK2 and compromised XopK-dependent virulence”
e1“rice somatic embryogenic receptor kinase 2 (OsSERK2)”
e2“Xanthomonas oryzae pv. oryzae”
The same questions in other papers
loss of function · Xanthomonas oryzae pv. oryzae · Oryza sativa (rice)Positive and negative results
10 papers in the same direction as this paper · 18 in the opposite direction
- no effect
- negative
- negative
25 papers more in this group
See all 29 papers →climate site · Xanthomonas oryzae pv. oryzae · Oryza sativa (rice)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.