Abstract
By using an in vivo hydroponic rice seedling culture system, we investigated the physiological and biochemical responses of a model rice japonica cultivar Nipponbare to salt stress using proteomics and classical biochemical methods. Yoshida's nutrient solution (YS) was used to grow rice seedlings. YS-grown 18-day-old seedlings manifested highly stable and reproducible symptoms, prominently the wilting and browning of the 3rd leaf, reduced photosynthetic activity, inhibition in overall seedling growth, and failure to develop new (5th) leaf, when subjected to salt stress by transferring them to YS containing 130 mM NaCl for 4 days. As leaf response to salt stress is least investigated in rice by proteomics, we used the 3rd leaf as source material. A comparison of 2-DE protein profiles between the untreated control and salt-stressed 3rd leaves revealed 55 differentially expressed CBB-stained spots, where 47 spots were increased over the control. Of these changed spots, the identity of 33 protein spots (27 increased and 5 decreased) was determined by nESI-LC-MS/MS. Most of these identified proteins belonged to major metabolic processes like photosynthetic carbon dioxide assimilation and photorespiration, suggesting a good correlation between salt stress-responsive proteins and leaf morphology. Moreover, 2-DE immunoblot and enzymatic activity analyses of 3rd leaves revealed remarkable changes in the key marker enzymes associated with oxidative damage to salt stress: ascorbate peroxidase and lipid peroxidation were induced, and catalase was suppressed. These results demonstrate that hydroponic culture system is best suited for proteomics of salt stress in rice seedling.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 14
Claim · model-written summary
Salt stressed seedlings showed wilting and browning of the third leaf.
- Taxon
- rice
Oryza sativae2 - Material
- Nipponbaree2
- Treatment
- YS containing 130 mM NaCl for 4 dayse1
- Outcome
- wilting and browning of the 3rd leafe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“wilting and browning of the 3rd leaf”
e1“when subjected to salt stress by transferring them to YS containing 130 mM NaCl for 4 days”
e2“a model rice japonica cultivar Nipponbare”
Claim · model-written summary
Salt stress reduced photosynthetic activity.
- Taxon
- riceOryza sativae2
- Material
- Nipponbaree2
- Treatment
- YS containing 130 mM NaCl for 4 dayse1
- Outcome
- photosynthetic activitye0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“reduced photosynthetic activity”
e1“when subjected to salt stress by transferring them to YS containing 130 mM NaCl for 4 days”
e2“a model rice japonica cultivar Nipponbare”
Claim · model-written summary
Salt stress inhibited overall seedling growth.
- Taxon
- riceOryza sativae2
- Material
- Nipponbaree2
- Treatment
- YS containing 130 mM NaCl for 4 dayse1
- Outcome
- overall seedling growthe0
- Direction
- inhibitione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inhibition in overall seedling growth”
e1“when subjected to salt stress by transferring them to YS containing 130 mM NaCl for 4 days”
e2“a model rice japonica cultivar Nipponbare”
Claim · model-written summary
Salt stressed seedlings failed to develop a new fifth leaf.
- Taxon
- riceOryza sativae2
- Material
- Nipponbaree2
- Treatment
- YS containing 130 mM NaCl for 4 dayse1
- Outcome
- failure to develop new (5th) leafe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“failure to develop new (5th) leaf”
e1“when subjected to salt stress by transferring them to YS containing 130 mM NaCl for 4 days”
e2“a model rice japonica cultivar Nipponbare”
Claim · model-written summary
Comparison of treated and control third leaves found 55 differentially expressed protein spots.
- Taxon
- riceOryza sativae1
- Material
- Nipponbaree1
- Organ / stage
- 3rd leavese0
- Outcome
- differentially expressed CBB-stained spotse0
- Value
- 55e0
- Comparator
- untreated controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“between the untreated control and salt-stressed 3rd leaves revealed 55 differentially expressed CBB-stained spots”
e1“a model rice japonica cultivar Nipponbare”
Claim · model-written summary
Of the differentially expressed spots, 47 increased over the control.
- Taxon
- riceOryza sativae1
- Material
- Nipponbaree1
- Outcome
- spotse0
- Direction
- increasede0
- Value
- 47 spotse0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“47 spots were increased over the control”
e1“a model rice japonica cultivar Nipponbare”
Claim · model-written summary
The identity of 33 changed protein spots was determined.
- Taxon
- riceOryza sativae1
- Outcome
- identity of 33 protein spotse0
- Value
- 33 protein spotse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the identity of 33 protein spots (27 increased and 5 decreased) was determined by nESI-LC-MS/MS”
e1“a model rice japonica cultivar Nipponbare”
Claim · model-written summary
Twenty seven identified protein spots increased.
- Taxon
- riceOryza sativae1
- Outcome
- protein spotse0
- Direction
- increasede0
- Value
- 27e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“33 protein spots (27 increased and 5 decreased)”
e1“a model rice japonica cultivar Nipponbare”
Claim · model-written summary
Five identified protein spots decreased.
- Taxon
- riceOryza sativae1
- Outcome
- protein spotse0
- Direction
- decreasede0
- Value
- 5e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“33 protein spots (27 increased and 5 decreased)”
e1“a model rice japonica cultivar Nipponbare”
Claim · model-written summary
Most identified proteins belonged to photosynthetic carbon dioxide assimilation and photorespiration.
- Taxon
- riceOryza sativae1
- Outcome
- major metabolic processes like photosynthetic carbon dioxide assimilation and photorespiratione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Most of these identified proteins belonged to major metabolic processes like photosynthetic carbon dioxide assimilation and photorespiration”
e1“a model rice japonica cultivar Nipponbare”
Claim · model-written summary
Ascorbate peroxidase was induced in salt stressed third leaves.
- Taxon
- riceOryza sativae3
- Organ / stage
- 3rd leavese1
- Treatment
- salt stresse2
- Gene
- ascorbate peroxidasee0
- Outcome
- ascorbate peroxidasee0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ascorbate peroxidase and lipid peroxidation were induced, and catalase was suppressed”
e1“of 3rd leaves revealed remarkable changes”
e2“salt stress by transferring them to YS containing 130 mM NaCl for 4 days”
e3“a model rice japonica cultivar Nipponbare”
Claim · model-written summary
Lipid peroxidation was induced in salt stressed third leaves.
- Taxon
- riceOryza sativae3
- Organ / stage
- 3rd leavese1
- Treatment
- salt stresse2
- Outcome
- lipid peroxidatione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ascorbate peroxidase and lipid peroxidation were induced, and catalase was suppressed”
e1“of 3rd leaves revealed remarkable changes”
e2“salt stress by transferring them to YS containing 130 mM NaCl for 4 days”
e3“a model rice japonica cultivar Nipponbare”
Claim · model-written summary
Catalase was suppressed in salt stressed third leaves.
- Taxon
- riceOryza sativae3
- Organ / stage
- 3rd leavese1
- Treatment
- salt stresse2
- Gene
- catalasee0
- Outcome
- catalasee0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ascorbate peroxidase and lipid peroxidation were induced, and catalase was suppressed”
e1“of 3rd leaves revealed remarkable changes”
e2“salt stress by transferring them to YS containing 130 mM NaCl for 4 days”
e3“a model rice japonica cultivar Nipponbare”
Claim · model-written summary
The hydroponic culture system was judged well suited to rice seedling salt stress proteomics.
- Taxon
- riceOryza sativae0
- Outcome
- proteomics of salt stress in rice seedlinge0
- Comparator
- best suitede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“hydroponic culture system is best suited for proteomics of salt stress in rice seedling”
The same questions in other papers
sodium chloride · growth · Oryza sativa (rice)Results differ
99 papers in the same direction as this paper · 28 in the opposite direction
- positive
- positive
- positive
124 papers more in this group
See all 128 papers →sodium chloride · disease · Oryza sativa (rice)Positive and negative results
2 papers in the same direction as this paper · 5 in the opposite direction
- positive
- positive
- positive
4 papers more in this group
See all 8 papers →sodium chloride · physiology: photosynthesis · Oryza sativa (rice)Positive and negative results
21 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- no effect
19 papers more in this group
See all 23 papers →fertilization · physiology: photosynthesis · Oryza sativa (rice)Positive and negative results
4 papers in the same direction as this paper · 78 in the opposite direction
- positive
- positive
- positive
79 papers more in this group
See all 83 papers →fertilization · disease · Oryza sativa (rice)Positive and negative results
23 papers in the same direction as this paper · 91 in the opposite direction
- positive
- positive
- positive
111 papers more in this group
See all 115 papers →fertilization · growth · Oryza sativa (rice)Positive and negative results
88 papers in the same direction as this paper · 670 in the opposite direction
- positive
- positive
- positive
755 papers more in this group
See all 759 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.