Abstract
In wheat (Triticum aestivum) roots, about 67% of the total activity of acid phosphatase was associated with the cell wall debris, and 35% of it was released from the wall by incubation with 0.8 M NaCl overnight. Three major cytoplasmic and two major wall-bound acid phosphatases were then separated by gel filtration on Sephadex G-75 and ion-exchange chromatography on carboxymethyl cellulose, and some of their characteristics were compared. They showed maximum activities in the same pH range (4.7–5.0) and had broad substrate specificities. They all showed high activity toward ADP. followed by ATP, glucose-6-phosphate, and creatine phosphate and much less activity toward AMP, glucose-1-phosphate, fructose-1,6-diphosphate, and ribose-5-phosphate. The wall-bound enzymes were more active to ADP and ATP than those of the cytoplasm. Mg 2+ , Ni 2+ , NaCN, and EDTA had no appreciable effects on the enzymatic activities. However, all enzymes were strongly inhibited by Hg 2+ and Fe 3+ and, to varied degrees, by Cu 2+ , Zn 2+ , Co 2+ , NaF. p-chloromercuribenzoate, and urea in that order. The molecular weights, estimated by sodium dodecylsulfate gel electrophoresis, ranged from 28 000 to 64 000.
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Claims 14
Claim · model-written summary
About 67% of root acid phosphatase activity was associated with cell wall debris.
- Taxon
- wheat
Triticum aestivume0 - Organ / stage
- rootse0
- Gene
- acid phosphatasee0
- Outcome
- total activity of acid phosphatasee0
- Value
- 67%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In wheat (Triticum aestivum) roots, about 67% of the total activity of acid phosphatase was associated with the cell wall debris”
Claim · model-written summary
Incubation with 0.8 M NaCl released 35% of wall-associated activity.
- Taxon
- wheatTriticum aestivume1
- Treatment
- incubation with 0.8 M NaCl overnighte0
- Outcome
- released from the walle0
- Direction
- releasede0
- Value
- 35%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“35% of it was released from the wall by incubation with 0.8 M NaCl overnight.”
e1“In wheat (Triticum aestivum) roots”
Claim · model-written summary
Three major cytoplasmic acid phosphatases were separated.
- Taxon
- wheatTriticum aestivume1
- Gene
- acid phosphatasese0
- Outcome
- Three major cytoplasmice0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three major cytoplasmic and two major wall-bound acid phosphatases were then separated”
e1“In wheat (Triticum aestivum) roots”
Claim · model-written summary
Two major wall-bound acid phosphatases were separated.
- Taxon
- wheatTriticum aestivume1
- Gene
- acid phosphatasese0
- Outcome
- two major wall-bound acid phosphatasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three major cytoplasmic and two major wall-bound acid phosphatases were then separated”
e1“In wheat (Triticum aestivum) roots”
Claim · model-written summary
The enzymes had maximal activity at pH 4.7–5.0.
- Taxon
- wheatTriticum aestivume1
- Outcome
- maximum activitiese0
- Value
- 4.7–5.0e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“They showed maximum activities in the same pH range (4.7–5.0)”
e1“In wheat (Triticum aestivum) roots”
Claim · model-written summary
The enzymes had broad substrate specificity.
- Taxon
- wheatTriticum aestivume1
- Outcome
- broad substrate specificitiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“had broad substrate specificities.”
e1“In wheat (Triticum aestivum) roots”
Claim · model-written summary
Activity was high toward ADP, followed by ATP, glucose-6-phosphate, and creatine phosphate.
- Taxon
- wheatTriticum aestivume1
- Outcome
- high activity toward ADP. followed by ATP, glucose-6-phosphate, and creatine phosphatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“They all showed high activity toward ADP. followed by ATP, glucose-6-phosphate, and creatine phosphate”
e1“In wheat (Triticum aestivum) roots”
Claim · model-written summary
Activity was much lower toward AMP, glucose-1-phosphate, fructose-1,6-diphosphate, and ribose-5-phosphate.
- Taxon
- wheatTriticum aestivume1
- Outcome
- activity toward AMP, glucose-1-phosphate, fructose-1,6-diphosphate, and ribose-5-phosphatee0
- Direction
- much lesse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“much less activity toward AMP, glucose-1-phosphate, fructose-1,6-diphosphate, and ribose-5-phosphate.”
e1“In wheat (Triticum aestivum) roots”
Claim · model-written summary
Wall-bound enzymes were more active toward ADP than cytoplasmic enzymes.
- Taxon
- wheatTriticum aestivume1
- Outcome
- active to ADPe0
- Direction
- more activee0
- Comparator
- cytoplasme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The wall-bound enzymes were more active to ADP and ATP than those of the cytoplasm.”
e1“In wheat (Triticum aestivum) roots”
Claim · model-written summary
Wall-bound enzymes were more active toward ATP than cytoplasmic enzymes.
- Taxon
- wheatTriticum aestivume1
- Outcome
- ATPe0
- Direction
- more activee0
- Comparator
- cytoplasme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The wall-bound enzymes were more active to ADP and ATP than those of the cytoplasm.”
e1“In wheat (Triticum aestivum) roots”
Claim · model-written summary
Mg 2+, Ni 2+, NaCN, and EDTA had no appreciable effect on enzyme activity.
- Taxon
- wheatTriticum aestivume1
- Treatment
- Mg 2+ , Ni 2+ , NaCN, and EDTAe0
- Outcome
- enzymatic activitiese0
- Direction
- no appreciable effectse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mg 2+ , Ni 2+ , NaCN, and EDTA had no appreciable effects on the enzymatic activities.”
e1“In wheat (Triticum aestivum) roots”
Claim · model-written summary
Hg 2+ and Fe 3+ strongly inhibited the enzymes.
- Taxon
- wheatTriticum aestivume1
- Treatment
- Hg 2+ and Fe 3+e0
- Outcome
- enzymese0
- Direction
- strongly inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all enzymes were strongly inhibited by Hg 2+ and Fe 3+”
e1“In wheat (Triticum aestivum) roots”
Claim · model-written summary
Cu 2+, Zn 2+, Co 2+, NaF, p-chloromercuribenzoate, and urea inhibited the enzymes to varying degrees.
- Taxon
- wheatTriticum aestivume2
- Treatment
- Cu 2+ , Zn 2+ , Co 2+ , NaF. p-chloromercuribenzoate, and ureae0
- Outcome
- enzymese1
- Direction
- inhibitede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“to varied degrees, by Cu 2+ , Zn 2+ , Co 2+ , NaF. p-chloromercuribenzoate, and urea in that order.”
e1“all enzymes were strongly inhibited by Hg 2+ and Fe 3+”
e2“In wheat (Triticum aestivum) roots”
Claim · model-written summary
Estimated molecular weights ranged from 28 000 to 64 000.
- Taxon
- wheatTriticum aestivume1
- Outcome
- molecular weightse0
- Value
- 28 000 to 64 000e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The molecular weights, estimated by sodium dodecylsulfate gel electrophoresis, ranged from 28 000 to 64 000.”
e1“In wheat (Triticum aestivum) roots”
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