Abstract
Abstract Defined media that promote the initiation and undifferentiated growth of callus derived from stem explants of four cultivars of cassava, Manihot esculenta Crantz, are described. Growth rates and yields of cassava callus after 4 weeks of culture are shown to be comparable to those of callus of Nicotiana tabacum L. cv. Wisconsin No. 38. Nitrogen sources of ammonium nitrate or of ammonium chloride plus succinate supported growth of all four cultivars. Sucrose was superior to glucose as a carbon source. The cassava cultivars differed in their response to increasing concentrations of sucrose between 0.5% (w/v) and 3%, two of them increasing in dry matter with increasing sucrose concentrations of up to 3%. When cultured in the light on defined media that contained higher ratios of cytokinin to auxin, callus of the latter two cultivars turned green. Roots but not shoots differentiated from the callus of all cultivars. The influence of hormone concentrations, sucrose level, and nitrogen source on greening and root formation is summarized.
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Claims 6
Claim · model-written summary
Growth rates and yields of cassava callus after 4 weeks were comparable to those of tobacco callus.
- Taxon
- cassava
Manihot esculentat0 - Outcome
- Growth rates and yields of cassava calluse1
- Comparator
- callus of Nicotiana tabacum L. cv. Wisconsin No. 38e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tissue Culture of Cassava”
e1“Growth rates and yields of cassava callus after 4 weeks of culture are shown to be comparable to those of callus of Nicotiana tabacum L. cv. Wisconsin No. 38”
Claim · model-written summary
Nitrogen sources of ammonium nitrate or ammonium chloride plus succinate supported callus growth of all four cultivars.
- Taxon
- cassavaManihot esculentat0
- Material
- all four cultivarse2
- Treatment
- Nitrogen sources of ammonium nitrate or of ammonium chloride plus succinatee2
- Outcome
- growthe2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tissue Culture of Cassava”
e2“Nitrogen sources of ammonium nitrate or of ammonium chloride plus succinate supported growth of all four cultivars”
Claim · model-written summary
Sucrose was superior to glucose as a carbon source for cassava callus.
- Taxon
- cassavaManihot esculentat0
- Treatment
- Sucrosee3
- Outcome
- superior to glucose as a carbon sourcee3
- Direction
- superiore3
- Comparator
- glucosee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tissue Culture of Cassava”
e3“Sucrose was superior to glucose as a carbon source”
Claim · model-written summary
Two cassava cultivars increased in callus dry matter with sucrose concentrations up to 3%.
- Taxon
- cassavaManihot esculentat0
- Treatment
- increasing sucrose concentrations of up to 3%e4
- Outcome
- dry mattere4
- Direction
- increasinge4
- Qualifier
- two of theme4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tissue Culture of Cassava”
e4“The cassava cultivars differed in their response to increasing concentrations of sucrose between 0.5% (w/v) and 3%, two of them increasing in dry matter with increasing sucrose concentrations of up to 3%”
Claim · model-written summary
Callus of the latter two cassava cultivars turned green on media with higher cytokinin to auxin ratios in the light.
- Taxon
- cassavaManihot esculentat0
- Material
- latter two cultivarse5
- Organ / stage
- calluse5
- Treatment
- higher ratios of cytokinin to auxine5
- Outcome
- turned greene5
- Setting
- in the lighte5
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tissue Culture of Cassava”
e5“When cultured in the light on defined media that contained higher ratios of cytokinin to auxin, callus of the latter two cultivars turned green”
Claim · model-written summary
Roots but not shoots differentiated from the callus of all cassava cultivars.
- Taxon
- cassavaManihot esculentat0
- Material
- all cultivarse6
- Organ / stage
- calluse6
- Outcome
- Roots but not shoots differentiatede6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tissue Culture of Cassava”
e6“Roots but not shoots differentiated from the callus of all cultivars”
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