Abstract
UV-B radiation and water deficit may trigger flavonol and anthocyanin biosynthesis in plant tissues. In addition, previous research has showed strong qualitative effects on grape berry skin flavonol and anthocyanin profiles in response to UV-B and water deficit. The aim of this study is to identify the mechanisms leading to quantitative and qualitative changes in flavonol and anthocyanin profiles, in response to separate and combined UV-B and water deficit. Grapevines (Vitis vinifera L. cv. Tempranillo) were exposed to three levels of UV-B radiation (0, 5.98 and 9.66 kJ m(-2) day(-1)) and subjected to two water regimes. A strong effect of UV-B on flavonol and anthocyanin biosynthesis was found, resulting in an increased anthocyanin concentration and a change in their profile. Concomitantly, two key biosynthetic genes (FLS1 and UFGT) were up-regulated by UV-B, leading to increased flavonol and anthocyanin skin concentration. Changes in flavonol and anthocyanin composition were explained to a large extend by transcript levels of F3'H, F3'5'H and OMT2. A significant interaction between UV-B and water deficit was found in the relative abundance of 3'4' and 3'4'5' substituted flavonols, but not in their anthocyanin homologues. The ratio between 3'4'5' and 3'4' substituted flavonols was linearly related to the ratios of F3'5'H and FLS1 transcription, two steps up-regulated independently by water deficit and UV-B radiation, respectively. Our results indicate that changes in flavonol profiles in response to environmental conditions are not only a consequence of changes in the expression of flavonoid hydroxylases; but also the result of the competition of FLS, F3'5'H and F3'H enzymes for the same flavonol substrates.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
UV-B increased anthocyanin concentration in grapevine berries.
VitisNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine Berries”
e0“a change in their profile”
e1“increased anthocyanin concentration”
e2“A strong effect of UV-B on flavonol and anthocyanin biosynthesis was found, resulting in an increased anthocyanin concentration and a change in their profile”
Claim · model-written summary
UV-B changed grapevine berry anthocyanin profiles.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine Berries”
e0“A strong effect of UV-B on flavonol and anthocyanin biosynthesis was found, resulting in an increased anthocyanin concentration and a change in their profile”
Claim · model-written summary
UV-B up-regulated FLS1 and UFGT transcription.
- Treatment
- UV-Be0
- Gene
- FLS1 and UFGTe0
- Outcome
- two key biosynthetic genes (FLS1 and UFGT)e0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine Berries”
e0“two key biosynthetic genes (FLS1 and UFGT) were up-regulated by UV-B”
Claim · model-written summary
UV-B increased flavonol and anthocyanin skin concentration.
- Organ / stage
- skine0
- Treatment
- UV-Be1
- Outcome
- flavonol and anthocyanin skin concentratione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine Berries”
e0“increased flavonol and anthocyanin skin concentration”
e1“by UV-B, leading to increased flavonol and anthocyanin skin concentration”
Claim · model-written summary
F3'H, F3'5'H and OMT2 transcript levels explained much of berry flavonoid composition.
- Gene
- F3'H, F3'5'H and OMT2e0
- Outcome
- flavonol and anthocyanin compositione0
- Qualifier
- to a large extende0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine Berries”
e0“Changes in flavonol and anthocyanin composition were explained to a large extend by transcript levels of F3'H, F3'5'H and OMT2”
Claim · model-written summary
UV-B and water deficit interacted in relative abundance of substituted flavonols.
- Treatment
- UV-B and water deficite0
- Outcome
- relative abundance of 3'4' and 3'4'5' substituted flavonolse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine Berries”
e0“A significant interaction between UV-B and water deficit was found in the relative abundance of 3'4' and 3'4'5' substituted flavonols”
Claim · model-written summary
The UV-B and water-deficit interaction was absent for anthocyanin homologues.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine Berries”
e0“A significant interaction between UV-B and water deficit was found in the relative abundance of 3'4' and 3'4'5' substituted flavonols, but not in their anthocyanin homologues”
Claim · model-written summary
The substituted flavonol ratio was linearly related to F3'5'H and FLS1 transcription ratios.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine Berries”
e0“The ratio between 3'4'5' and 3'4' substituted flavonols was linearly related to the ratios of F3'5'H and FLS1 transcription”
Claim · model-written summary
Water deficit independently up-regulated F3'5'H transcription.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine Berries”
e0“F3'5'H and FLS1 transcription, two steps up-regulated independently by water deficit and UV-B radiation, respectively”
Claim · model-written summary
Authors attribute flavonol profile changes partly to substrate competition among FLS, F3'5'H and F3'H.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine Berries”
e0“changes in flavonol profiles in response to environmental conditions are not only a consequence of changes in the expression of flavonoid hydroxylases; but also the result of the competition of FLS, F3'5'H and F3'H enzymes for the same flavonol substrates”
The same questions in other papers
uv · molecular: expression · VitisPositive and negative results
6 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
5 papers more in this group
See all 9 papers →uv · quality: phytochemicals · VitisPositive and negative results
13 papers in the same direction as this paper · 5 in the opposite direction
- no effect
- negative
- negative
15 papers more in this group
See all 19 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.