Abstract
BACKGROUND: Ultraviolet (UV) radiation modulates secondary metabolism in the skin of Vitis vinifera L. berries, which affects the final composition of both grapes and wines. The expression of several phenylpropanoid biosynthesis-related genes is regulated by UV radiation in grape berries. However, the complete portion of transcriptome and ripening processes influenced by solar UV radiation in grapes remains unknown. RESULTS: Whole genome arrays were used to identify the berry skin transcriptome modulated by the UV radiation received naturally in a mid-altitude Tempranillo vineyard. UV radiation-blocking and transmitting filters were used to generate the experimental conditions. The expression of 121 genes was significantly altered by solar UV radiation. Functional enrichment analysis of altered transcripts mainly pointed out that secondary metabolism-related transcripts were induced by UV radiation including VvFLS1, VvGT5 and VvGT6 flavonol biosynthetic genes and monoterpenoid biosynthetic genes. Berry skin phenolic composition was also analysed to search for correlation with gene expression changes and UV-increased flavonols accumulation was the most evident impact. Among regulatory genes, novel UV radiation-responsive transcription factors including VvMYB24 and three bHLH, together with known grapevine UV-responsive genes such as VvMYBF1, were identified. A transcriptomic meta-analysis revealed that genes up-regulated by UV radiation in the berry skin were also enriched in homologs of Arabidopsis UVR8 UV-B photoreceptor-dependent UV-B -responsive genes. Indeed, a search of the grapevine reference genomic sequence identified UV-B signalling pathway homologs and among them, VvHY5-1, VvHY5-2 and VvRUP were up-regulated by UV radiation in the berry skin. CONCLUSIONS: Results suggest that the UV-B radiation-specific signalling pathway is activated in the skin of grapes grown at mid-altitudes. The biosynthesis and accumulation of secondary metabolites, which are appreciated in winemaking and potentially confer cross-tolerance, were almost specifically triggered. This draws attention to viticultural practices that increase solar UV radiation on vineyards as they may improve grape features.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
Solar UV altered expression of 121 berry skin genes.
- Taxon
- Vitis vinifera L.
Vitis viniferae1 - Treatment
- solar UV radiatione0
- Outcome
- expression of 121 genese0
- Direction
- alterede0
- Value
- 121 genese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of 121 genes was significantly altered by solar UV radiation.”
e1“Vitis vinifera L. berries”
Claim · model-written summary
UV induced secondary metabolism transcripts, including flavonol biosynthesis genes.
- Treatment
- UV radiatione0
- Gene
- VvFLS1, VvGT5 and VvGT6e0
- Outcome
- secondary metabolism-related transcriptse0
- Direction
- inducede0
VitisNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“secondary metabolism-related transcripts were induced by UV radiation including VvFLS1, VvGT5 and VvGT6 flavonol biosynthetic genes and monoterpenoid biosynthetic genes.”
t0“grapevine”
Claim · model-written summary
UV increased flavonol accumulation in berry skin.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“UV-increased flavonols accumulation was the most evident impact.”
t0“grapevine”
Claim · model-written summary
UV-responsive transcription factors VvMYB24 and three bHLH were identified.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“novel UV radiation-responsive transcription factors including VvMYB24 and three bHLH, together with known grapevine UV-responsive genes such as VvMYBF1, were identified.”
Claim · model-written summary
UV-upregulated berry skin genes were enriched in homologs of Arabidopsis UVR8 dependent genes.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genes up-regulated by UV radiation in the berry skin were also enriched in homologs of Arabidopsis UVR8 UV-B photoreceptor-dependent UV-B -responsive genes.”
t0“grapevine”
Claim · model-written summary
Grapevine UV-B signalling homologs were identified in the reference genome.
- Outcome
- UV-B signalling pathway homologse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a search of the grapevine reference genomic sequence identified UV-B signalling pathway homologs”
Claim · model-written summary
UV upregulated VvHY5-1, VvHY5-2 and VvRUP in berry skin.
- Treatment
- UV radiatione0
- Gene
- VvHY5-1, VvHY5-2 and VvRUPe0
- Outcome
- VvHY5-1, VvHY5-2 and VvRUPe0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“VvHY5-1, VvHY5-2 and VvRUP were up-regulated by UV radiation in the berry skin.”
t0“grapevine”
Claim · model-written summary
Authors suggest UV-B signalling is activated in mid-altitude grape skin.
Verbatim
e0“the UV-B radiation-specific signalling pathway is activated in the skin of grapes grown at mid-altitudes.”
Claim · model-written summary
Authors suggest viticultural practices increasing UV may improve grape features.
Verbatim
e0“viticultural practices that increase solar UV radiation on vineyards as they may improve grape features.”
The same questions in other papers
radiation · molecular: expression · VitisPositive and negative results
1 in the opposite direction
- negative
uv · molecular: expression · VitisPositive and negative results
6 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
5 papers more in this group
See all 9 papers →uv · molecular: metabolites · VitisPositive and negative results
19 papers in the same direction as this paper · 5 in the opposite direction · 1 reporting no difference from an alternative
- negative
- positive
- positive
22 papers more in this group
See all 26 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.