Paper · 2000 · Journal of the American Society for Horticultural Science

Characterization of Phytoavailable Copper in Compost-Peat Substrates and Determination of a Toxicity Level

Annette S. Bucher; Manfred K. Schenk

17
claims
35
verbatim quotes
12
citations
3
research areas
✓
Open access

Abstract

Heavy metal-sensitive `Express Orchid' petunias ( Petunia × hybrida Hort Vilm.-Andr. `Express Orchid') were grown in substrates of 2 green yard waste compost: 3 peat (v/v) with target Cu contents of 100 and 200 mg·kg -1 at varying pH. Iron supply was also varied. Copper contents of the substrate were determined by H 2 O, NH 4 NO 3 , NH 4 OAc, CaCl 2 , CaCl 2 -DTPA, and aqua regia extraction. Plant Cu concentration increased with increasing Cu supply and decreasing pH, indicating that Cu phytoavailability depended on substrate pH. Extraction of fresh substrates with CaCl 2 -DTPA provided a good prediction of plant Cu concentration and reflected well the influence of pH on Cu phytoavailability. The percentage of CaCl 2 -DTPA extractable Cu increased with decreasing pH. Extractions of Cu with NH 4 NO 3 , H 2 O, NH 4 OAc, and CaCl 2 resulted in very low extractable amounts and hence were not suitable. Plants showed Cu toxicity induced iron-like deficiency chlorosis, which was alleviated by additional Fe supply. This Fe supply did not seem to affect total Fe concentration of petunias, but reduced Cu concentration of the shoots. Since yield reduction was not observed, the occurrence of chlorosis during the culture period was chosen as the toxicity parameter, resulting in a Cu threshold toxicity level of 12.3 mg . kg -1 plant dry weight. From this, a threshold toxicity level for CaCl 2 -DTPA extractable Cu in compost-peat substrates of 3 mg . L -1 substrate was determined. Chemical name used: diethylenetriamine-pentaacetic acid (DTPA).

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Claims 17

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Petunia copper concentration rose with increasing copper supply.

Taxon
petuniasPetuniae1
Treatment
increasing Cu supplye0
Outcome
Plant Cu concentratione0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia
Petunia · Taxon · picture from its Wikimedia Commons categorySalwa Farwaneh Dameh · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Plant Cu concentration increased with increasing Cu supply and decreasing pH”

✓ located verbatim in the source · abstract, characters 395–471 · show in text ↑
e1

“petunias ( Petunia × hybrida”

✓ located verbatim in the source · abstract, characters 39–67 · show in text ↑
Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

Petunia copper concentration rose as pH fell.

Taxon
petuniasPetuniae1
Treatment
decreasing pHe0
Outcome
Plant Cu concentratione0
Direction
increasede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia
Petunia · Taxon · picture from its Wikimedia Commons categorySalwa Farwaneh Dameh · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Plant Cu concentration increased with increasing Cu supply and decreasing pH”

✓ located verbatim in the source · abstract, characters 395–471 · show in text ↑
e1

“petunias ( Petunia × hybrida”

✓ located verbatim in the source · abstract, characters 39–67 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

CaCl 2 -DTPA extraction of fresh substrates predicted plant copper concentration.

Taxon
petuniasPetuniae1
Outcome
prediction of plant Cu concentratione0
Qualifier
good predictione0

Not reportedComparatorValue

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia
Petunia · Taxon · picture from its Wikimedia Commons categorySalwa Farwaneh Dameh · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Extraction of fresh substrates with CaCl 2 -DTPA provided a good prediction of plant Cu concentration and reflected well the influence of pH on Cu phytoavailability.”

✓ located verbatim in the source · abstract, characters 536–701 · show in text ↑
e1

“petunias ( Petunia × hybrida”

✓ located verbatim in the source · abstract, characters 39–67 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

CaCl 2 -DTPA extraction reflected pH effects on copper phytoavailability.

Taxon
petuniasPetuniae1
Outcome
influence of pH on Cu phytoavailabilitye0

Not reportedComparatorValue

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia
Petunia · Taxon · picture from its Wikimedia Commons categorySalwa Farwaneh Dameh · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Extraction of fresh substrates with CaCl 2 -DTPA provided a good prediction of plant Cu concentration and reflected well the influence of pH on Cu phytoavailability.”

✓ located verbatim in the source · abstract, characters 536–701 · show in text ↑
e1

“petunias ( Petunia × hybrida”

✓ located verbatim in the source · abstract, characters 39–67 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

The CaCl 2 -DTPA extractable copper percentage increased as pH decreased.

Taxon
petuniasPetuniae1
Treatment
decreasing pHe0
Outcome
percentage of CaCl 2 -DTPA extractable Cue0
Direction
increasede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia
Petunia · Taxon · picture from its Wikimedia Commons categorySalwa Farwaneh Dameh · CC0 · Wikimedia Commons ↗

Verbatim

e0

“The percentage of CaCl 2 -DTPA extractable Cu increased with decreasing pH.”

✓ located verbatim in the source · abstract, characters 702–777 · show in text ↑
e1

“petunias ( Petunia × hybrida”

✓ located verbatim in the source · abstract, characters 39–67 · show in text ↑
Method / resourceobservedoriginal resultnegativemeasured

Claim · model-written summary

NH 4 NO 3 extraction yielded very low extractable copper and was unsuitable.

Taxon
petuniasPetuniae1
Outcome
very low extractable amountse0
Qualifier
not suitablee0

Not reportedComparatorValue

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia
Petunia · Taxon · picture from its Wikimedia Commons categorySalwa Farwaneh Dameh · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Extractions of Cu with NH 4 NO 3 , H 2 O, NH 4 OAc, and CaCl 2 resulted in very low extractable amounts and hence were not suitable.”

✓ located verbatim in the source · abstract, characters 778–910 · show in text ↑
e1

“petunias ( Petunia × hybrida”

✓ located verbatim in the source · abstract, characters 39–67 · show in text ↑
Method / resourceobservedoriginal resultnegativemeasured

Claim · model-written summary

H 2 O extraction yielded very low extractable copper and was unsuitable.

Taxon
petuniasPetuniae1
Outcome
very low extractable amountse0

Not reportedComparatorValue

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia
Petunia · Taxon · picture from its Wikimedia Commons categorySalwa Farwaneh Dameh · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Extractions of Cu with NH 4 NO 3 , H 2 O, NH 4 OAc, and CaCl 2 resulted in very low extractable amounts and hence were not suitable.”

✓ located verbatim in the source · abstract, characters 778–910 · show in text ↑
e1

“petunias ( Petunia × hybrida”

✓ located verbatim in the source · abstract, characters 39–67 · show in text ↑
Method / resourceobservedoriginal resultnegativemeasured

Claim · model-written summary

NH 4 OAc extraction yielded very low extractable copper and was unsuitable.

Taxon
petuniasPetuniae1
Outcome
very low extractable amountse0

Not reportedComparatorValue

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia
Petunia · Taxon · picture from its Wikimedia Commons categorySalwa Farwaneh Dameh · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Extractions of Cu with NH 4 NO 3 , H 2 O, NH 4 OAc, and CaCl 2 resulted in very low extractable amounts and hence were not suitable.”

✓ located verbatim in the source · abstract, characters 778–910 · show in text ↑
e1

“petunias ( Petunia × hybrida”

✓ located verbatim in the source · abstract, characters 39–67 · show in text ↑
Method / resourceobservedoriginal resultnegativemeasured

Claim · model-written summary

CaCl 2 extraction yielded very low extractable copper and was unsuitable.

Taxon
petuniasPetuniae1
Outcome
very low extractable amountse0

Not reportedComparatorValue

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia
Petunia · Taxon · picture from its Wikimedia Commons categorySalwa Farwaneh Dameh · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Extractions of Cu with NH 4 NO 3 , H 2 O, NH 4 OAc, and CaCl 2 resulted in very low extractable amounts and hence were not suitable.”

✓ located verbatim in the source · abstract, characters 778–910 · show in text ↑
e1

“petunias ( Petunia × hybrida”

✓ located verbatim in the source · abstract, characters 39–67 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Copper toxicity produced iron deficiency-like chlorosis in petunias.

Taxon
petuniasPetuniae1
Compound
Cu toxicitye0
Outcome
iron-like deficiency chlorosise0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia
Petunia · Taxon · picture from its Wikimedia Commons categorySalwa Farwaneh Dameh · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Plants showed Cu toxicity induced iron-like deficiency chlorosis”

✓ located verbatim in the source · abstract, characters 911–975 · show in text ↑
e1

“petunias ( Petunia × hybrida”

✓ located verbatim in the source · abstract, characters 39–67 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Additional iron supply alleviated chlorosis.

Taxon
petuniasPetuniae2
Treatment
additional Fe supplye0
Outcome
chlorosise1
Direction
alleviatede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia
Petunia · Taxon · picture from its Wikimedia Commons categorySalwa Farwaneh Dameh · CC0 · Wikimedia Commons ↗

Verbatim

e0

“which was alleviated by additional Fe supply.”

✓ located verbatim in the source · abstract, characters 977–1022 · show in text ↑
e1

“Plants showed Cu toxicity induced iron-like deficiency chlorosis”

✓ located verbatim in the source · abstract, characters 911–975 · show in text ↑
e2

“petunias ( Petunia × hybrida”

✓ located verbatim in the source · abstract, characters 39–67 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Additional iron appeared not to affect total iron concentration.

Taxon
petuniasPetuniae0
Treatment
Fe supplye0
Outcome
total Fe concentratione0
Direction
did not seem to affecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia
Petunia · Taxon · picture from its Wikimedia Commons categorySalwa Farwaneh Dameh · CC0 · Wikimedia Commons ↗

Verbatim

e0

“This Fe supply did not seem to affect total Fe concentration of petunias”

✓ located verbatim in the source · abstract, characters 1023–1095 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Iron supply reduced shoot copper concentration.

Taxon
petuniasPetuniae1
Treatment
Fe supplye2
Outcome
Cu concentration of the shootse0
Direction
reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia
Petunia · Taxon · picture from its Wikimedia Commons categorySalwa Farwaneh Dameh · CC0 · Wikimedia Commons ↗

Verbatim

e0

“but reduced Cu concentration of the shoots.”

✓ located verbatim in the source · abstract, characters 1097–1140 · show in text ↑
e2

“which was alleviated by additional Fe supply.”

✓ located verbatim in the source · abstract, characters 977–1022 · show in text ↑
e1

“petunias ( Petunia × hybrida”

✓ located verbatim in the source · abstract, characters 39–67 · show in text ↑
Observationobservedoriginal resultno effectmeasured

Claim · model-written summary

No yield reduction was observed under these copper conditions.

Taxon
petuniasPetuniae1
Outcome
yield reductione0
Direction
not observede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia
Petunia · Taxon · picture from its Wikimedia Commons categorySalwa Farwaneh Dameh · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Since yield reduction was not observed”

✓ located verbatim in the source · abstract, characters 1141–1179 · show in text ↑
e1

“petunias ( Petunia × hybrida”

✓ located verbatim in the source · abstract, characters 39–67 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Chlorosis during cultivation was chosen as the copper toxicity parameter.

Taxon
petuniasPetuniae1
Outcome
occurrence of chlorosis during the culture periode0
Value
12.3 mg . kg -1 plant dry weighte0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia
Petunia · Taxon · picture from its Wikimedia Commons categorySalwa Farwaneh Dameh · CC0 · Wikimedia Commons ↗

Verbatim

e0

“the occurrence of chlorosis during the culture period was chosen as the toxicity parameter, resulting in a Cu threshold toxicity level of 12.3 mg . kg -1 plant dry weight.”

✓ located verbatim in the source · abstract, characters 1181–1352 · show in text ↑
e1

“petunias ( Petunia × hybrida”

✓ located verbatim in the source · abstract, characters 39–67 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The plant dry weight copper toxicity threshold was 12.3 mg per kg.

Taxon
petuniasPetuniae1
Outcome
Cu threshold toxicity levele0
Value
12.3 mg . kg -1 plant dry weighte0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia
Petunia · Taxon · picture from its Wikimedia Commons categorySalwa Farwaneh Dameh · CC0 · Wikimedia Commons ↗

Verbatim

e0

“a Cu threshold toxicity level of 12.3 mg . kg -1 plant dry weight”

✓ located verbatim in the source · abstract, characters 1286–1351 · show in text ↑
e1

“petunias ( Petunia × hybrida”

✓ located verbatim in the source · abstract, characters 39–67 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

The substrate CaCl 2 -DTPA extractable copper toxicity threshold was 3 mg per liter.

Taxon
petuniasPetuniae1
Outcome
threshold toxicity level for CaCl 2 -DTPA extractable Cu in compost-peat substratese0
Value
3 mg . L -1 substratee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Petunia
Petunia · Taxon · picture from its Wikimedia Commons categorySalwa Farwaneh Dameh · CC0 · Wikimedia Commons ↗

Verbatim

e0

“a threshold toxicity level for CaCl 2 -DTPA extractable Cu in compost-peat substrates of 3 mg . L -1 substrate was determined.”

✓ located verbatim in the source · abstract, characters 1364–1490 · show in text ↑
e1

“petunias ( Petunia × hybrida”

✓ located verbatim in the source · abstract, characters 39–67 · show in text ↑

copper atom · nutrient: content · Petunia (petunia)Results agree

2 papers: 0 positive, 2 negative or no effect

1 paper in the same direction as this paper

  1. no effect
See all 2 papers →

iron atom · nutrient: content · Petunia (petunia)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
    High-limestone substrate without Fe and Mn lowered leaf Fe and Mn content relative to controls.

    Occurrence and correction of lime-induced chlorosis in petunia plants · 2011 · compared with control plants in the R3 substrate

    Same directionAbout lacking this nutrient, so it counts the other way round

See all 3 papers →

iron atom · physiology: pigments · Petunia (petunia)Results agree

5 papers: 5 positive, 0 negative or no effect

4 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.