Abstract
An attempt was made to standardize in vitro clonal propagation of pomegranate cv. Bhagwa, using nodal segment and shoot tip explants at e, India. Direct organogenesis of pomegranate was tried in MS medium with growth regulator combinations viz., benzyl amino purine (BAP), α-naphthalene acetic acid (NAA) and adenine sulphate for in vitro shoot regeneration and indole butyric acid (IBA) for in vitro rooting. Among the explants evaluated, nodal segments responded better for culture establishment. Pre-treatment of nodal segments with 0.5% carbendazim for 30 min followed by surface sterilization with 70% ethanol for 30 sec and 0.5% sodium hypochlorite for 10 min gave highest survival rate (81.7%) with minimum microbial contamination (13.3%). Phenolic exudation was reduced by treating explants in antioxidant solution consisting of citric acid (100 mg L-1) and ascorbic acid (150 mg L-1) for 20 min and then cultured on basal MS medium with polyvinyl pyrrolidone (100 mg L-1) resulted in maximum survival (98.3%). The earliest shoot evocation response (4.4 days), higher shoot proliferation (91.7%) and maximum shoot number (4.6) were observed on basal MS medium with adenine sulphate (40 mg L1). Half strength MS medium supplemented with IBA (1 mg L-1), recorded minimum days to root initiation (16.9) and maximum rooting percentage (71.7%) with highernumber of roots explant-1(3.7) and lengthier roots (3.93 cm). Rooted plantlets transferred to hardening media with pot mixture and vermicompost (1:1) exhibited an ex-vitro survival of 81.7%.
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Claims 13
Claim · model-written summary
Nodal segments responded better than other explants for culture establishment.
- Taxon
- Pomegranate
Punica granatumt0 - Organ / stage
- nodal segmentse0
- Outcome
- culture establishmente0
- Direction
- bettere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among the explants evaluated, nodal segments responded better for culture establishment”
t0“Pomegranate”
Claim · model-written summary
Carbendazim and sterilization pretreatment yielded 81.7% survival.
- Taxon
- PomegranatePunica granatumt0
- Treatment
- Pre-treatment of nodal segments with 0.5% carbendazim for 30 min followed by surface sterilization with 70% ethanol for 30 sec and 0.5% sodium hypochlorite for 10 mine0
- Outcome
- survival ratee0
- Direction
- higheste0
- Value
- 81.7%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pre-treatment of nodal segments with 0.5% carbendazim for 30 min followed by surface sterilization with 70% ethanol for 30 sec and 0.5% sodium hypochlorite for 10 min gave highest survival rate (81.7%) with minimum microbial contamination (13.3%)”
t0“Pomegranate”
Claim · model-written summary
The same pretreatment yielded minimum microbial contamination of 13.3%.
- Taxon
- PomegranatePunica granatumt0
- Treatment
- Pre-treatment of nodal segments with 0.5% carbendazim for 30 min followed by surface sterilization with 70% ethanol for 30 sec and 0.5% sodium hypochlorite for 10 mine0
- Outcome
- microbial contaminatione0
- Direction
- minimume0
- Value
- 13.3%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pre-treatment of nodal segments with 0.5% carbendazim for 30 min followed by surface sterilization with 70% ethanol for 30 sec and 0.5% sodium hypochlorite for 10 min gave highest survival rate (81.7%) with minimum microbial contamination (13.3%)”
t0“Pomegranate”
Claim · model-written summary
Antioxidant treatment reduced phenolic exudation.
- Taxon
- PomegranatePunica granatumt0
- Treatment
- antioxidant solution consisting of citric acid (100 mg L-1) and ascorbic acid (150 mg L-1) for 20 mine0
- Outcome
- Phenolic exudatione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phenolic exudation was reduced by treating explants in antioxidant solution consisting of citric acid (100 mg L-1) and ascorbic acid (150 mg L-1) for 20 min”
t0“Pomegranate”
Claim · model-written summary
Antioxidant treatment followed by culture with polyvinyl pyrrolidone gave 98.3% survival.
- Taxon
- PomegranatePunica granatumt0
- Treatment
- antioxidant solution consisting of citric acid (100 mg L-1) and ascorbic acid (150 mg L-1) for 20 min and then cultured on basal MS medium with polyvinyl pyrrolidone (100 mg L-1)e0
- Outcome
- survivale0
- Direction
- maximume0
- Value
- 98.3%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treating explants in antioxidant solution consisting of citric acid (100 mg L-1) and ascorbic acid (150 mg L-1) for 20 min and then cultured on basal MS medium with polyvinyl pyrrolidone (100 mg L-1) resulted in maximum survival (98.3%)”
t0“Pomegranate”
Claim · model-written summary
MS medium with adenine sulphate yielded earliest shoot evocation at 4.4 days.
- Taxon
- PomegranatePunica granatumt0
- Treatment
- basal MS medium with adenine sulphate (40 mg L1)e0
- Outcome
- shoot evocation responsee0
- Direction
- earlieste0
- Value
- 4.4 dayse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The earliest shoot evocation response (4.4 days), higher shoot proliferation (91.7%) and maximum shoot number (4.6) were observed on basal MS medium with adenine sulphate (40 mg L1)”
t0“Pomegranate”
Claim · model-written summary
MS medium with adenine sulphate yielded 91.7% shoot proliferation.
- Taxon
- PomegranatePunica granatumt0
- Treatment
- basal MS medium with adenine sulphate (40 mg L1)e0
- Outcome
- shoot proliferatione0
- Direction
- highere0
- Value
- 91.7%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The earliest shoot evocation response (4.4 days), higher shoot proliferation (91.7%) and maximum shoot number (4.6) were observed on basal MS medium with adenine sulphate (40 mg L1)”
t0“Pomegranate”
Claim · model-written summary
MS medium with adenine sulphate yielded maximum shoot number of 4.6.
- Taxon
- PomegranatePunica granatumt0
- Treatment
- basal MS medium with adenine sulphate (40 mg L1)e0
- Outcome
- shoot numbere0
- Direction
- maximume0
- Value
- 4.6e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The earliest shoot evocation response (4.4 days), higher shoot proliferation (91.7%) and maximum shoot number (4.6) were observed on basal MS medium with adenine sulphate (40 mg L1)”
t0“Pomegranate”
Claim · model-written summary
Half-strength MS medium with IBA minimized time to root initiation at 16.9 days.
- Taxon
- PomegranatePunica granatumt0
- Treatment
- Half strength MS medium supplemented with IBA (1 mg L-1)e0
- Outcome
- days to root initiatione0
- Direction
- minimume0
- Value
- 16.9e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Half strength MS medium supplemented with IBA (1 mg L-1), recorded minimum days to root initiation (16.9) and maximum rooting percentage (71.7%) with highernumber of roots explant-1(3.7) and lengthier roots (3.93 cm)”
t0“Pomegranate”
Claim · model-written summary
Half-strength MS medium with IBA gave 71.7% rooting.
- Taxon
- PomegranatePunica granatumt0
- Treatment
- Half strength MS medium supplemented with IBA (1 mg L-1)e0
- Outcome
- rooting percentagee0
- Direction
- maximume0
- Value
- 71.7%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Half strength MS medium supplemented with IBA (1 mg L-1), recorded minimum days to root initiation (16.9) and maximum rooting percentage (71.7%) with highernumber of roots explant-1(3.7) and lengthier roots (3.93 cm)”
t0“Pomegranate”
Claim · model-written summary
Half-strength MS medium with IBA gave 3.7 roots per explant.
- Taxon
- PomegranatePunica granatumt0
- Treatment
- Half strength MS medium supplemented with IBA (1 mg L-1)e0
- Outcome
- number of roots explant-1e0
- Direction
- highere0
- Value
- 3.7e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Half strength MS medium supplemented with IBA (1 mg L-1), recorded minimum days to root initiation (16.9) and maximum rooting percentage (71.7%) with highernumber of roots explant-1(3.7) and lengthier roots (3.93 cm)”
t0“Pomegranate”
Claim · model-written summary
Half-strength MS medium with IBA produced roots 3.93 cm long.
- Taxon
- PomegranatePunica granatumt0
- Treatment
- Half strength MS medium supplemented with IBA (1 mg L-1)e0
- Outcome
- rootse0
- Direction
- lengthiere0
- Value
- 3.93 cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Half strength MS medium supplemented with IBA (1 mg L-1), recorded minimum days to root initiation (16.9) and maximum rooting percentage (71.7%) with highernumber of roots explant-1(3.7) and lengthier roots (3.93 cm)”
t0“Pomegranate”
Claim · model-written summary
Rooted plantlets in pot mixture and vermicompost survived ex vitro at 81.7%.
- Taxon
- PomegranatePunica granatumt0
- Treatment
- hardening media with pot mixture and vermicompost (1:1)e0
- Outcome
- ex-vitro survivale0
- Value
- 81.7%e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rooted plantlets transferred to hardening media with pot mixture and vermicompost (1:1) exhibited an ex-vitro survival of 81.7%”
t0“Pomegranate”
The same questions in other papers
ascorbic acid · quality: phytochemicals · Punica granatum (pomegranate)Results agree
1 paper in the same direction as this paper
- positive
sodium hypochlorite · survival · Punica granatum (pomegranate)Results agree
1 paper in the same direction as this paper
- positive
adenine · growth · Punica granatum (pomegranate)Results agree
1 paper in the same direction as this paper
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sulfate · growth · Punica granatum (pomegranate)Results agree
1 paper in the same direction as this paper
- positive
indole-3-butyric acid · growth · Punica granatum (pomegranate)Results agree
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- no effect
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See all 25 papers →fertilization · survival · Punica granatum (pomegranate)Results agree
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- positive
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.