Abstract
Magnesium (Mg)-mediated alleviation of aluminum (Al) toxicity has been observed in a number of plant species, but the mechanisms underlying the alleviation are still poorly understood. When a putative rice (Oryza sativa) Mg transporter gene, Oryza sativa MAGNESIUM TRANSPORTER1 (OsMGT1), was knocked out, the tolerance to Al, but not to cadmium and lanthanum, was decreased. However, this inhibition could be rescued by addition of 10 μm Mg, but not by the same concentration of barium or strontium. OsMGT1 was expressed in both the roots and shoots in the absence of Al, but the expression only in the roots was rapidly up-regulated by Al. Furthermore, the expression did not respond to low pH and other metals including cadmium and lanthanum, and was regulated by an Al-responsive transcription factor, AL RESISTANCE TRANSCRIPTION FACTOR1. An investigation of subcellular localization showed that OsMGT1 was localized to the plasma membrane. A short-term (30 min) uptake experiment with stable isotope (25)Mg showed that knockout of OsMGT1 resulted in decreased Mg uptake, but that the uptake in the wild type was enhanced by Al. Mg concentration in the cell sap of the root tips was also increased in the wild-type rice, but not in the knockout lines in the presence of Al. A microarray analysis showed that transcripts of genes related to stress were more up- and down-regulated in the knockout lines. Taken together, our results indicate that OsMGT1 is a transporter for Mg uptake in the roots and that up-regulation of this gene is required for conferring Al tolerance in rice by increasing Mg concentration in the cell.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 12
Claim · model-written summary
OsMGT1 knockout reduced aluminum tolerance but not cadmium or lanthanum tolerance.
- Taxon
- rice
Oryza sativae0 - Treatment
- knocked oute0
- Gene
- OsMGT1e0
- Outcome
- tolerance to Ale0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When a putative rice (Oryza sativa) Mg transporter gene, Oryza sativa MAGNESIUM TRANSPORTER1 (OsMGT1), was knocked out, the tolerance to Al, but not to cadmium and lanthanum, was decreased”
Claim · model-written summary
Adding 10 μm Mg rescued the inhibition caused by OsMGT1 knockout; barium and strontium did not.
- Taxon
- RiceOryza sativat0
- Treatment
- addition of 10 μm Mge0
- Outcome
- this inhibitione0
- Direction
- rescuede0
- Comparator
- barium or strontiume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, this inhibition could be rescued by addition of 10 μm Mg, but not by the same concentration of barium or strontium”
t0“Rice”
Claim · model-written summary
OsMGT1 was expressed in roots and shoots without aluminum.
- Taxon
- RiceOryza sativat0
- Organ / stage
- roots and shootse0
- Gene
- OsMGT1e0
- Outcome
- expressede0
- Qualifier
- absence of Ale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsMGT1 was expressed in both the roots and shoots in the absence of Al”
t0“Rice”
Claim · model-written summary
Aluminum rapidly up-regulated OsMGT1 expression in roots.
- Taxon
- RiceOryza sativat0
- Organ / stage
- rootse0
- Treatment
- Ale0
- Gene
- OsMGT1t1
- Outcome
- expressione0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression only in the roots was rapidly up-regulated by Al”
t0“Rice”
t1“OsMGT1”
Claim · model-written summary
OsMGT1 expression did not respond to low pH, cadmium, or lanthanum.
- Taxon
- RiceOryza sativat0
- Treatment
- low pH and other metals including cadmium and lanthanume0
- Gene
- OsMGT1t1
- Outcome
- expressione0
- Direction
- did not responde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression did not respond to low pH and other metals including cadmium and lanthanum”
t0“Rice”
t1“OsMGT1”
Claim · model-written summary
OsMGT1 expression was regulated by AL RESISTANCE TRANSCRIPTION FACTOR1.
- Taxon
- RiceOryza sativat0
- Gene
- AL RESISTANCE TRANSCRIPTION FACTOR1e0
- Outcome
- regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was regulated by an Al-responsive transcription factor, AL RESISTANCE TRANSCRIPTION FACTOR1”
t0“Rice”
Claim · model-written summary
OsMGT1 localized to the plasma membrane.
- Taxon
- RiceOryza sativat0
- Gene
- OsMGT1e0
- Outcome
- localized to the plasma membranee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsMGT1 was localized to the plasma membrane”
t0“Rice”
Claim · model-written summary
OsMGT1 knockout decreased magnesium uptake.
- Taxon
- RiceOryza sativat0
- Treatment
- knockout of OsMGT1e0
- Gene
- OsMGT1e0
- Outcome
- Mg uptakee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“knockout of OsMGT1 resulted in decreased Mg uptake”
t0“Rice”
Claim · model-written summary
Aluminum enhanced magnesium uptake in wild-type rice.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the uptake in the wild type was enhanced by Al”
t0“Rice”
Claim · model-written summary
Aluminum increased magnesium concentration in root-tip cell sap of wild type but not knockout rice.
- Taxon
- riceOryza sativae0
- Material
- wild-type ricee0
- Treatment
- Ale0
- Outcome
- Mg concentration in the cell sap of the root tipse0
- Direction
- increasede0
- Comparator
- knockout linese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mg concentration in the cell sap of the root tips was also increased in the wild-type rice, but not in the knockout lines in the presence of Al”
Claim · model-written summary
Stress-related transcripts showed greater up- and down-regulation in knockout lines.
- Taxon
- RiceOryza sativat0
- Material
- knockout linese0
- Outcome
- transcripts of genes related to stresse0
- Direction
- more up- and down-regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcripts of genes related to stress were more up- and down-regulated in the knockout lines”
t0“Rice”
Claim · model-written summary
The authors conclude that OsMGT1 mediates root magnesium uptake and that its up-regulation supports aluminum tolerance by raising cellular magnesium.
- Taxon
- riceOryza sativae0
- Organ / stage
- rootse0
- Gene
- OsMGT1e0
- Outcome
- Al tolerancee0
- Qualifier
- increasing Mg concentration in the celle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsMGT1 is a transporter for Mg uptake in the roots and that up-regulation of this gene is required for conferring Al tolerance in rice by increasing Mg concentration in the cell”
The same questions in other papers
cadmium atom · molecular: expression · Oryza sativa (rice)Positive and negative results
7 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
6 papers more in this group
See all 10 papers →aluminium atom · molecular: expression · Oryza sativa (rice)Positive and negative results
7 papers in the same direction as this paper · 6 in the opposite direction
- positive
- positive
- no effect
10 papers more in this group
See all 14 papers →ph · molecular: expression · Oryza sativa (rice)Results agree
3 papers in the same direction as this paper
- negative
- no effect
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.